Relación entre factores socioculturales y percepción sobre el aborto de la adolescente de la institución Educativa Maria Negron Ugarte Trujillo, 2014

El presente estudio descriptivo correlacional, se desarrolló en el mes de mayo del 2014, con la finalidad de determinar la relación entre los factores socioculturales y percepción de la adolescente sobre el aborto en la Institución María Negrón Ugarte. Trujillo 2014. La muestra estuvo constituida por 210 adolescentes entre 12 y 19 años, de quien se obtuvieron los datos a través de la aplicación del cuestionario sobre factores socioculturales y percepción sobre el aborto de la adolescente, llegando a la conclusión que el 95.2 % de adolescentes no están de acuerdo con el aborto, no obteniéndose una relación significativa entre los factores socioculturales estudiados: edad, grado de estudio, tipo de familia, religión, y lugar de procedencia con la percepción de la adolescente sobre el aborto.This correlational descriptive study was conducted in May 2014 in order to determine the relationship between sociocultural factors and perceptions of teen's abortion Institution Maria Negron Ugarte. Trujillo 2014. The sample consisted of 210 adolescents aged 12 to 19, from whom the data were obtained through the application of the questionnaire on socio-cultural factors and perception of abortion teen, concluding that 95.2% of adolescents are not according to the abortion, not yielding a significant relationship between the studied sociocultural factors: age, level of education, type of family, religion, and place of origin to the perception of the teen about abortion.Tesi

Aplicación de estreptoquinasa recombinante en el Infarto Agudo al Miocardio. Application of recombinant streptokinase in Acute Myocardial Infarction

Se estudiaron 74 pacientes (55 masculinos y 19 del sexo femenino) con una edad promedio de 61,2 ± 14,5 desviación standard que ingresaron de Enero de 1998 a Mayo de 2000 en el hospital "León Cuervo Rubio" de Pinar del Río con el diagnóstico de infarto agudo del miocardio (IMA) y a los que se le realizó trombólisis intravenosa siguiendo los criterios de inclusión del protocolo multicentro nacional del grupo cubano para la aplicación de la estreptocinasa recombinante (ER) en el IMA. Se usó la dosis de 1,5 millones de UI de ER (Heberkinasa), administrados al paciente una sola vez a través de una vena periférica en el transcurso de 60 minutos. El 66.2% de los pacientes eran fumadores, 35.1% eran hipertensos y 17 pacientes eran hipertensos y fumadores. En 8 (10,8 %) pacientes existía el antecedente de infarto miocárdico previo. La aplicación de la ER fue completa en 67 pacientes (90,5 %) con un tiempo entre el inicio de los síntomas y la aplicación del fármaco menor de 7 horas en el 66,2 % de los casos. 67 pacientes tenían IMA-Q, de ellos en cara anterior 34 (45,9%), inferior en 25 (33,8%), múltiple en 6 (8,1%) y 2 (2,7%) anteroseptales. Se presentó fiebre en el 18,9 % de los pacientes, hipotensión en el 12,2 %, sangramiento en 3 pacientes y un AVE hemorrágico fatal confirmado por la necropsia. El (6,8%) hizo alergia ligera a la ER con rash cutáneo, vómitos, náuseas, escalofríos y bochorno facial. Shock anafiláctico no fatales en 2 pacientes (2,7%). El reinfarto se diagnosticó en 2 pacientes y la insuficiencia cardiaca en 16 (21,6%). La letalidad intrahospitalaria fue 8,1% DeCS: INFARTO DEL MIOCARDIO/quimioterapia, ESTREPTOCINASA/uso terapéutico. ABSTRACT Seventy four patients are studied (55 males and 19 females) with a mean age of 61.2 ±14.5 Standard deviation (SD) and they were admitted at León Cuervo Rubio Hospital Pinar del Río between January 1998 and May 2000 diagnosed as having acute myocardial infarction and they underwent intravenous thrombosis following the inclusion criteria of the national multicenter protocol of the Cuban group for the use of recombinant streptokinase in acute myocardial. A dose of 1.5 millions of Heberkinase IU is used through a peripheral vein given only one time during 60 minutes. 66.2 % of patients were smokers, 31.1 % suffered from hypertension and 17 patients suffered from hypertension and they also were smokers. Acute myocardial infarction was present in 8 patients (10.8 %) previously. The administration of ER was complete in 67 patients (90.5 %) with a lapse of time between the initial symptoms and the administrations of drugs less than 7 hours in 66.2 % of cases. Sixty seven patients were diagnosed as having AMI - Q presenting 34 (45.9 %) in anterior side, 25 in lower side (33.8 %), multiple in 6 (8.1 %) and 2 (2.7 %) anteroseptal side. Fever was present in 18.9 % of patients, hypotension was observed in 12.2 % of patients, bleeding in 3 patients and a lethal hemorrhagic vascular encephalic accident documented by necropsy. Mild allergy to ER was observed in 6.8 % presenting rash, vomits, nauseas, chills and facial redness and non - lethal anaphylactic shock in 2 patients (2.7 %). Re - infarction was diagnosed in 2 patients and heart failure in 16 (21.6 %). Intra - hospital mortality rate was 8.1 %. DeCS: MYOCARDIAL INFARCTION/treatment, STREPTOCINASA

Aplicación de estreptoquinasa recombinante en el Infarto Agudo al Miocardio. Application of recombinant streptokinase in Acute Myocardial Infarction

Se estudiaron 74 pacientes (55 masculinos y 19 del sexo femenino) con una edad promedio de 61,2 ± 14,5 desviación standard que ingresaron de Enero de 1998 a Mayo de 2000 en el hospital "León Cuervo Rubio" de Pinar del Río con el diagnóstico de infarto agudo del miocardio (IMA) y a los que se le realizó trombólisis intravenosa siguiendo los criterios de inclusión del protocolo multicentro nacional del grupo cubano para la aplicación de la estreptocinasa recombinante (ER) en el IMA. Se usó la dosis de 1,5 millones de UI de ER (Heberkinasa), administrados al paciente una sola vez a través de una vena periférica en el transcurso de 60 minutos. El 66.2% de los pacientes eran fumadores, 35.1% eran hipertensos y 17 pacientes eran hipertensos y fumadores. En 8 (10,8 %) pacientes existía el antecedente de infarto miocárdico previo. La aplicación de la ER fue completa en 67 pacientes (90,5 %) con un tiempo entre el inicio de los síntomas y la aplicación del fármaco menor de 7 horas en el 66,2 % de los casos. 67 pacientes tenían IMA-Q, de ellos en cara anterior 34 (45,9%), inferior en 25 (33,8%), múltiple en 6 (8,1%) y 2 (2,7%) anteroseptales. Se presentó fiebre en el 18,9 % de los pacientes, hipotensión en el 12,2 %, sangramiento en 3 pacientes y un AVE hemorrágico fatal confirmado por la necropsia. El (6,8%) hizo alergia ligera a la ER con rash cutáneo, vómitos, náuseas, escalofríos y bochorno facial. Shock anafiláctico no fatales en 2 pacientes (2,7%). El reinfarto se diagnosticó en 2 pacientes y la insuficiencia cardiaca en 16 (21,6%). La letalidad intrahospitalaria fue 8,1% DeCS: INFARTO DEL MIOCARDIO/quimioterapia, ESTREPTOCINASA/uso terapéutico. ABSTRACT Seventy four patients are studied (55 males and 19 females) with a mean age of 61.2 ±14.5 Standard deviation (SD) and they were admitted at León Cuervo Rubio Hospital Pinar del Río between January 1998 and May 2000 diagnosed as having acute myocardial infarction and they underwent intravenous thrombosis following the inclusion criteria of the national multicenter protocol of the Cuban group for the use of recombinant streptokinase in acute myocardial. A dose of 1.5 millions of Heberkinase IU is used through a peripheral vein given only one time during 60 minutes. 66.2 % of patients were smokers, 31.1 % suffered from hypertension and 17 patients suffered from hypertension and they also were smokers. Acute myocardial infarction was present in 8 patients (10.8 %) previously. The administration of ER was complete in 67 patients (90.5 %) with a lapse of time between the initial symptoms and the administrations of drugs less than 7 hours in 66.2 % of cases. Sixty seven patients were diagnosed as having AMI - Q presenting 34 (45.9 %) in anterior side, 25 in lower side (33.8 %), multiple in 6 (8.1 %) and 2 (2.7 %) anteroseptal side. Fever was present in 18.9 % of patients, hypotension was observed in 12.2 % of patients, bleeding in 3 patients and a lethal hemorrhagic vascular encephalic accident documented by necropsy. Mild allergy to ER was observed in 6.8 % presenting rash, vomits, nauseas, chills and facial redness and non - lethal anaphylactic shock in 2 patients (2.7 %). Re - infarction was diagnosed in 2 patients and heart failure in 16 (21.6 %). Intra - hospital mortality rate was 8.1 %. DeCS: MYOCARDIAL INFARCTION/treatment, STREPTOCINASA

Genetic Polymorphisms of Interleukin-1 Alpha and the Vitamin D Receptor in Mexican Mestizo Patients with Intervertebral Disc Degeneration

Intervertebral disc degeneration (IDD) is the most common diagnosis in patients with back pain, a leading cause of musculoskeletal disability worldwide. Several conditions, such as occupational activities, gender, age, and obesity, have been associated with IDD. However, the development of this disease has strong genetic determinants. In this study, we explore the possible association between rs1800587 (c.-949C>T) of interleukin-1 alpha (IL1A) and rs2228570 (c.2T>V) and rs731236 (c.1056T>C) of vitamin D receptor (VDR) gene polymorphisms and the development of IDD in northwestern Mexican Mestizo population. Gene polymorphisms were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism, in two groups matched by age and gender: patients with symptomatic lumbar IDD (n = 100) and subjects with normal lumbar-spine MRI-scans (n = 100). Distribution of the mutated alleles in patients and controls was 27.0% versus 28.0% (P = 0.455) for T of rs1800587 (IL1A); 53.0% versus 58.0% (P = 0.183) for V of rs2228570 (VDR); and 18.0% versus 21.0% (P = 0.262) for C of rs731236 (VDR). Our results showed no association between the studied polymorphisms and IDD in this population. This is the first report on the contribution of gene polymorphisms on IDD in a Mexican population

Circulating circRNA as biomarkers for dilated cardiomyopathy etiology

Dilated cardiomyopathy (DCM) is the third most common cause of heart failure. The multidisciplinary nature of testing - involving genetics, imaging, or cardiovascular techniques - makes its diagnosis challenging. Novel and reliable biomarkers are needed for early identification and tailored personalized management. Peripheral circular RNAs (circRNAs), a leading research topic, remain mostly unexplored in DCM. We aimed to assess whether peripheral circRNAs are expressed differentially among etiology-based DCM. The study was based on a case-control multicentric study. We enrolled 130 subjects: healthy controls (n = 20), idiopathic DCM (n = 30), ischemic DCM (n = 20), and familial DCM patients which included pathogen variants of (i) LMNA gene (n = 30) and (ii) BCL2-associated athanogene 3 (BAG3) gene (n = 30). Differentially expressed circRNAs were analyzed in plasma samples by quantitative RT-PCR and correlated to relevant systolic and diastolic parameters. The pathophysiological implications were explored through bioinformatics tools. Four circRNAs were overexpressed compared to controls: hsa_circ_0003258, hsa_circ_0051238, and hsa_circ_0051239 in LMNA-related DCM and hsa_circ_0089762 in the ischemic DCM cohort. The obtained areas under the curve confirm the discriminative capacity of circRNAs. The circRNAs correlated with some diastolic and systolic echocardiographic parameters with notable diagnostic potential in DCM. Circulating circRNAs may be helpful for the etiology-based diagnosis of DCM as a non-invasive biomarker. Key messages The limitations of cardiac diagnostic imaging and the absence of a robust biomarker reveal the need for a diagnostic tool for dilated cardiomyopathy (DCM). The circular RNA (circRNA) expression pattern is paramount for categorizing the DCM etiologies. Our peripheral circRNAs fingerprint discriminates between various among etiology-based DCM and correlates with some echocardiographic parameters. We provide a potential non-invasive biomarker for the etiology-based diagnosis of LMNA-related DCM and ischemic DCM.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by grants in the framework of the European Regional Development Fund (ERDF) Integrated Territorial Initiative (ITI PI0048-2017 and ITI0033_2019), a clinical research grant from the Spanish Society of Cardiology for Basic Research in cardiology (PI0012_2019), COST (European Cooperation in Science and Technology) Action EUCardioRNA CA17129, and the Portuguese Foundation for Science and Technology (FCT) under the framework of the research grant PTDC-MED-GEN-29389-2017

Kernicterus by glucose-6-phosphate dehydrogenase deficiency: a case report and review of the literature

<p>Abstract</p> <p>Introduction</p> <p>Glucose-6-phosphate dehydrogenase deficiency is an X-linked recessive disease that causes acute or chronic hemolytic anemia and potentially leads to severe jaundice in response to oxidative agents. This deficiency is the most common human innate error of metabolism, affecting more than 400 million people worldwide.</p> <p>Case presentation</p> <p>Here, we present the first documented case of kernicterus in Panama, in a glucose-6-phosphate dehydrogenase-deficient newborn clothed in naphthalene-impregnated garments, resulting in reduced psychomotor development, neurosensory hypoacousia, absence of speech and poor reflex of the pupil to light.</p> <p>Conclusion</p> <p>Mutational analysis revealed the glucose-6-phosphate dehydrogenase Mediterranean polymorphic variant, which explained the development of kernicterus after exposition of naphthalene. As the use of naphthalene in stored clothes is a common practice, glucose-6-phosphate dehydrogenase testing in neonatal screening could prevent severe clinical consequences.</p

El cuento de terror.

Editors: Ambròs Pallarès, Alba i Ramos Sabaté, Joan MarcLos trabajos que se presentan pertenecen a dos grupos de alumnas que cursaron la asignatura “Planificación y evaluación de la Lengua y la Literatura en EGB (8ª a 10ª) y Bachillerato” en el Máster de formación de profesorado de Educación Secundaria en Ecuador en el verano de 2017 en Quito. Son dos ejemplos de éxito de la tarea que les encomendamos: la realización de una secuencia didáctica con un enfoque comunicativo. La primera secuencia se titula "Escribamos un microcuento" y la segunda "El cuento de terror"

Organotypic 3D Cell-Architecture Impacts the Expression Pattern of miRNAs–mRNAs Network in Breast Cancer SKBR3 Cells

Background. Currently, most of the research on breast cancer has been carried out in conventional two-dimensional (2D) cell cultures due to its practical benefits, however, the three-dimensional (3D) cell culture is becoming the model of choice in cancer research because it allows cell–cell and cell–extracellular matrix (ECM) interactions, mimicking the native microenvironment of tumors in vivo. Methods. In this work, we evaluated the effect of 3D cell organization on the expression pattern of miRNAs (by Small-RNAseq) and mRNAs (by microarrays) in the breast cancer SKBR3 cell line and analyzed the biological processes and signaling pathways regulated by the differentially expressed protein-coding genes (DE-mRNAs) and miRNAs (DE-microRNAs) found in the organoids. Results. We obtained well-defined cell-aggregated organoids with a grape cluster-like morphology with a size up to 9.2 × 105 μm3. The transcriptomic assays showed that cell growth in organoids significantly affected (all p < 0.01) the gene expression patterns of both miRNAs, and mRNAs, finding 20 upregulated and 19 downregulated DE-microRNAs, as well as 49 upregulated and 123 downregulated DE-mRNAs. In silico analysis showed that a subset of 11 upregulated DE-microRNAs target 70 downregulated DE-mRNAs. These genes are involved in 150 gene ontology (GO) biological processes such as regulation of cell morphogenesis, regulation of cell shape, regulation of canonical Wnt signaling pathway, morphogenesis of epithelium, regulation of cytoskeleton organization, as well as in the MAPK and AGE–RAGE signaling KEGG-pathways. Interestingly, hsa-mir-122-5p (Fold Change (FC) = 15.4), hsa-mir-369-3p (FC = 11.4), and hsa-mir-10b-5p (FC = 20.1) regulated up to 81% of the 70 downregulated DE-mRNAs. Conclusion. The organotypic 3D cell-organization architecture of breast cancer SKBR3 cells impacts the expression pattern of the miRNAs–mRNAs network mainly through overexpression of hsa-mir-122-5p, hsa-mir-369-3p, and hsa-mir-10b-5p. All these findings suggest that the interaction between cell–cell and cell–ECM as well as the change in the culture architecture impacts gene expression, and, therefore, support the pertinence of migrating breast cancer research from conventional cultures to 3D models

Health-related quality of life with palbociclib plus endocrine therapy versus capecitabine in postmenopausal patients with hormone receptor–positive metastatic breast cancer: Patient-reported outcomes in the PEARL study

Background: The PEARL study showed that palbociclib plus endocrine therapy (palbociclib/ET) was not superior to capecitabine in improving progression-free survival in postmenopausal patients with metastatic breast cancer resistant to aromatase inhibitors, but was better tolerated. This analysis compared patient-reported outcomes. Patients and methods: The PEARL quality of life (QoL) population comprised 537 patients, 268 randomised to palbociclib/ET (exemestane or fulvestrant) and 269 to capecitabine. Patients completed the European Organisation for Research and Treatment of Cancer QLQC30 and QLQ-BR23 and EQ-5D-3L questionnaires. Changes from the baseline and time to deterioration (TTD) were analysed using linear mixed-effect and stratified Cox regression models, respectively. Results: Questionnaire completion rate was high and similar between treatment arms. Significant differences were observed in the mean change in global health status (GHS)/QoL scores from the baseline to cycle 3 (2.9 for palbociclib/ET vs.-2.1 for capecitabine (95% confidence interval [CI], 1.4-8.6; P = 0.007). The median TTD in GHS/QoL was 8.3 months for palbociclib/ET versus 5.3 months for capecitabine (adjusted hazard ratio, 0.70; 95% CI, 0.55-0.89; P = 0.003). Similar improvements for palbociclib/ET were also seen for other scales as physical, role, cognitive, social functioning, fatigue, nausea/vomiting and appetite loss. No differences were observed between the treatment arms in change from the baseline in any item of the EQ-5D-L3 questionnaire as per the overall index score and visual analogue scale. Conclusion: Patients receiving palbociclib/ET experienced a significant delay in deterioration of GHS/QoL and several functional and symptom scales compared with capecitabine, providing additional evidence that palbociclib/ET is better tolerated. Trial registration number: NCT02028507 (ClinTrials.gov). EudraCT study number: 2013-003170-27. 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Crónicas de Jurisprudencia. Derechos Fundamentales y Libertades Púlbicas

Crónica de la jurisprudencia sobre derechos y libertades de los siguientes tribunales: Tribunal Europeo de Derechos Humanos, Tribunal de Justicia de la Unión Europea, Tribunal Constitucional, Tribunal SupremoFUNDACIÓ BOSCH I GIMPERA y Editorial Aranzadi (Thomson Reuters)2020-2