7 research outputs found
Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis
Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naĂŻve T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model
Fibroblast sources: Where can we get them?
Fibroblasts are cells widely used in cell culture, both for transient primary cell culture or permanent as transformed cell lines. Lately, fibroblasts become cell sources for use in disease modeling after cell reprogramming because it is easily accessible in the body. Fibroblasts in patients will maintain all genetic background during reprogramming into induced pluripotent stem cells. In spite of their large use, fibroblasts are obtained after an invasive procedure, a superficial punch skin biopsy, collected under patient's local anesthesia. Taking into consideration the minimum patient's discomfort during and after the biopsy procedure, as well as the aesthetics aspect, it is essential to reflect on the best site of the body for the biopsy procedure combined with the success of getting robust fibroblast cultures in the lab. For this purpose, we compared the efficiency of four biopsy sites of the body (skin from eyelid, back of the ear, abdominal cesarean scar and groin). Cell proliferation assays and viability after cryopreservation were measured. Our results revealed that scar tissue provided fibroblasts with higher proliferative rates. Also, fibroblasts from scar tissues presented a higher viability after the thawing process
Manifestações orais associada ao papilomavĂrus humano (hpv) conceitos atuais: revisĂŁo bibliográfica Oral manifestations related to papillomavirus (hpv)
O papilomavĂrus (HPV) Ă© um DNA vĂrus do grupo papovavĂrus, que Ă© altamente transmissĂvel sexualmente, sendo freqĂĽente na regiĂŁo ano-genital e raro na mucosa oral. A sua implantação oral pode ser por auto-inoculação ou pelo contato oro-sexual. As manifestações orais associadas ao HPV sĂŁo: papiloma, condiloma acuminado, verruga vulgar, hiperplasia epitelial focal, leucoplasias, lĂquen plano e carcinoma. O diagnĂłstico Ă© dado pelo exame da lesĂŁo e confirmado pela biĂłpsia, com a identificação do tipo de HPV pelas tĂ©cnicas de biologia molecular (captura hĂbrida e PCR). O tratamento, dependendo da lesĂŁo, pode ser clĂnico e/ou cirĂşrgico, obtendo assim a cura clĂnica, pois o vĂrus permanece no epitĂ©lio da mucosa mesmo apĂłs o tratamento.<br>The human papillomavirus (HPV) is a DNA virus, of the papovavirus group, that is highly sexually transmittable. It is common in the anal and genital parts and rarely in the oral mucosa. The oral implantation can be by self-inoculation or by oral-sexual contact. The oral manifestations related to HPV are: papilloma, condyloma acuminatum, verruca vulgaris, focal epithelial hyperplasia, leukoplasia, lichen planus, and the squamous cell carcinoma. The diagnosis is performed by lesion exam and confirmed by biopsy, showing the HPV genotype by molecular biology techniques (hybrid capture and PCR). The treatment, depending on the lesion, can be clinical or surgical, allowing clinical cure, because the virus remains in the epithelium of the mucosa even after the treatment
Yeast killer toxin-like candidacidal Ab6 antibodies elicited through the manipulation of the idiotypic cascade
A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces
anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall
receptors (KTR) mainly constituted by beta-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following
immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). MAb K10 was produced by immunization with a KTneutralizing
mAb (mAb KT4, Ab1) bearing the internal image of KTR. MAb K20, likewise mAb K10, proved to be fungicidal in
vitro against KT-sensitive Candida albicans cells, an activity neutralized by mAb KT4, and was capable of binding to beta-1,3-
glucan. MAb K20 and mAb K10 competed with each other and with KT for binding to C. albicans KTR. MAb K20 was used to
identify peptide mimics of KTR by the selection of phage clones from random peptide phage display libraries. Using this
strategy, four peptides (TK 1-4) were selected and used as immunogen in mice in the form of either keyhole limpet
hemocyanin (KLH) conjugates or peptide-encoding minigenes. Peptide and DNA immunization could induce serum Abs
characterized by candidacidal activity, which was inhibited by laminarin, a soluble beta-1,3-glucan, but not by pustulan, a beta-1,6-
glucan. These findings show that the idiotypic cascade can not only overcome the barrier of animal species but also the
nature of immunogens and the type of technology adopted