Sodium 2-mercaptoethanesulfonate monohydrate (coenzyme M sodium salt monohydrate)

The 2-thio­ethanesulfonate anion is the smallest known coenzyme in nature (HS–CoM) and plays a key role in methano­genesis by anaerobic archaea, as well as in the oxidation of alkenes by Gram-negative and Gram-positive eubacteria. The title compound, Na+·C2H5O3S2 −·H2O, is the Na+ salt of HS–CoM crystallized as the monohydrate. Six O atoms form a distorted octa­hedral coordination geometry around the Na atom, at distances in the range 2.312 (4)–2.517 (3) Å. Two O atoms of the sulfonate group, one O atom of each of three other symmetry-related sulfonate groups plus the water O atom form the coordination environment of the Na+ ion. This arrangement forms Na–O–Na layers in the crystal structure, parallel to (100)

Prüfung und Entwicklung von Sojabohnenzuchtmaterial für die Fütterung mit dem Ziel der Implementierung mittelständischer Züchtungsaktivitäten unter besonderer Berücksichtigung der Merkmale Frühzeitigkeit, Kältetoleranz und Proteinertrag

Mit dem Vorhaben soll im Rahmen der Eiweißstrategie des BMEL durch die Stärkung der Züchtungswirtschaft eine Ausweitung und Verbesserung des Sojabohnenanbaus und damit der Produktion von gentechnikfreiem, heimischem Eiweißfutter unterstützt werden. An der Landesanstalt wird ein Soja-Zuchtprogramm neu aufgebaut. Im Verbund mit privaten bayerischen Züchtungsunternehmen werden Methoden zur Selektion von Zuchtstämmen bezüglich der Merkmale Frühreife, Kornertrag, Proteinertrag und Kältetoleranz entwickelt, sowie die Grundlage für eine markergestützte Selektion geschaffen. In den drei Vegetationsperioden (2015-2017) wurden aus über zwölftausend Kreuzungsnachkommen interessante Zuchtstämme selektiert und in einer Leistungsprüfung mit aktuellen Sorten in Ertrag, Proteingehalt und Abreife verglichen. Dabei konnten mehrere Zuchtstämme identifiziert werden, die bei hohem Ertrag früher abreifen als aktuelle Vergleichssorten bzw. höhere Proteinerträge liefern. Das entwickelte Zuchtmaterial fand Eingang in die Zuchtprogramme bei den privaten Verbundpartnern und wird auf die Sortenzulassung vorbereitet. Die molekulargenetischen Analysen auf der Basis von 6000 SNP-Markern zeigten eine nur geringe genetische Diversität im aktuell genutzten Sortenpanel. Die in Clusteranalysen nachgewiesene große genetische Diversität in weltweiten Genbankakzessionen kann als wertvoller Genpool für die Züchtung genutzt werden. Die aufwändige phänotypische Selektion soll langfristig durch molekulare Marker effizienter gestaltet werden. Der erste Schritt wurde mit der Etablierung und Anwendung publizierter Marker für die Merkmale Blüte und Reife gemacht

Development and validation of molecular markers tagging anthracnose resistance in white lupin (Lupinus albus, L.)

White lupin (Lupinus albus, L.) has great potential to be utilized more in organic and sustainable agricultural systems. However, there are major obstacles in the production of this crop to overcome. One of these is the susceptibility against its main fungal pathogen, Colletotrichum lupini, which causes anthracnose disease, representing a serious threat to lupin cultivation worldwide, potentially leading to total yield loss in Swiss growing conditions. The trait of anthracnose resistance is polygenically controlled in white lupin, no complete resistance was observed and only few sources of resistance were identified until now. Efforts are constantly made to breed cultivars with increased resistance. To achieve this goal, developing and validating molecular markers for direct application of marker assisted selection (MAS) and genomic selection (GS) in variety development is pivotal

Genome-wide association study revealed significant SNPs for anthracnose resistance, seed alkaloids and protein content in white lupin

White lupin (Lupinus albus L.) is a high-protein grain legume alternative to soybean in Central Europe, but its cultivation is risky due to the fungal disease anthracnose that can cause severe yield damage. In addition, management of seed alkaloids is critical for human nutrition and animal feed. We report on a white lupin collection of genebank accessions, advanced breeding lines and cultivars that was genotyped and phenotypically characterized for anthracnose resistance and seed alkaloids and protein levels. Using genotyping by sequencing (GBS), SeqSNP-targeted GBS, BiomarkX genotyping and Sanger sequencing, a genetic resource of genome-wide SNPs for white lupin was established. We determined anthracnose resistance in two years field trials at four locations with infection rows and measured seed alkaloids and protein levels by near-infrared spectroscopy (NIRS). Few white lupin breeding lines showed anthracnose resistance comparable or better than Celina and Frieda, currently the best commercial cultivars in Germany. NIRS estimates for seed alkaloids and protein levels revealed variation in the white lupin collection. Using genome-wide association studies (GWAS), we identified SNPs significantly associated with anthracnose resistance in the field representing known and new genomic regions. We confirmed the pauper locus and detected new SNP markers significantly associated with seed alkaloids. For the first time, we present loci associated with total grain protein content. Finally, we tested the potential of genomic prediction (GP) in predicting the phenotype of these three quantitative traits. Application of results and resources are discussed in the context of fostering breeding programs for white lupin

A balanced chromosomal translocation disrupting ARHGEF9 is associated with epilepsy, anxiety, aggression, and mental retardation

Clustering of inhibitory -aminobutyric acidA (GABAA) and glycine receptors at synapses is thought to involve key interactions between the receptors, a scaffolding protein known as gephyrin and the RhoGEF collybistin. We report the identification of a balanced chromosomal translocation in a female patient presenting with a disturbed sleep-wake cycle, late-onset epileptic seizures, increased anxiety, aggressive behavior, and mental retardation, but not hyperekplexia. Fine mapping of the breakpoint indicates disruption of the collybistin gene (ARHGEF9) on chromosome Xq11, while the other breakpoint lies in a region of 18q11 that lacks any known or predicted genes. We show that defective collybistin transcripts are synthesized and exons 7-10 are replaced by cryptic exons from chromosomes X and 18. These mRNAs no longer encode the pleckstrin homology (PH) domain of collybistin, which we now show binds phosphatidylinositol-3-phosphate (PI3P/PtdIns-3-P), a phosphoinositide with an emerging role in membrane trafficking and signal transduction, rather than phosphatidylinositol 3,4,5-trisphosphate (PIP3/PtdIns-3,4,5-P) as previously suggested in the membrane activation model of gephyrin clustering. Consistent with this finding, expression of truncated collybistin proteins in cultured neurons interferes with synaptic localization of endogenous gephyrin and GABAA receptors. These results suggest that collybistin has a key role in membrane trafficking of gephyrin and selected GABAA receptor subtypes involved in epilepsy, anxiety, aggression, insomnia, and learning and memory

Characterisation of barley resistance to rhynchosporium on chromosome 6HS

Key Message: Major resistance gene to rhynchosporium, Rrs18, maps close to the telomere on the short arm of chromosome 6H in barley. Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S₂ and S₃ lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.Max Coulter, Bianca Büttner, Kerstin Hofmann, Micha Bayer, Luke Ramsay, Günther Schweizer, Robbie Waugh, Mark E. Looseley, Anna Avrov

Searching for VHE gamma-ray emission associated with IceCube neutrino alerts using FACT, H.E.S.S., MAGIC, and VERITAS

The realtime follow-up of neutrino events is a promising approach to searchfor astrophysical neutrino sources. It has so far provided compelling evidencefor a neutrino point source: the flaring gamma-ray blazar TXS 0506+056 observedin coincidence with the high-energy neutrino IceCube-170922A detected byIceCube. The detection of very-high-energy gamma rays (VHE, $\mathrm{E} >100\,\mathrm{GeV}$) from this source helped establish the coincidence andconstrained the modeling of the blazar emission at the time of the IceCubeevent. The four major imaging atmospheric Cherenkov telescope arrays (IACTs) -FACT, H.E.S.S., MAGIC, and VERITAS - operate an active follow-up program oftarget-of-opportunity observations of neutrino alerts sent by IceCube. Thisprogram has two main components. One are the observations of known gamma-raysources around which a cluster of candidate neutrino events has been identifiedby IceCube (Gamma-ray Follow-Up, GFU). Second one is the follow-up of singlehigh-energy neutrino candidate events of potential astrophysical origin such asIceCube-170922A. GFU has been recently upgraded by IceCube in collaborationwith the IACT groups. We present here recent results from the IACT follow-upprograms of IceCube neutrino alerts and a description of the upgraded IceCubeGFU system.<br

Tabriz (Nordwest-Iran) und der Tabrizer Bazar

Donated by Klaus Kreise

Ostpontisches Gebirge

Donated by Klaus Kreise