Spawning season and larval occurrence of blood cockle (Anadara granosa) off the Selangor coast, Peninsular Malaysia

We performed a plankton survey of blood cockle larvae, Anadara spp., and histological observations of the gonads of adult blood cockle, Anadara granosa, in the coastal waters off Sungai Buloh, Peninsular Malaysia from October 2010 to April 2011. The histological observations indicated the main spawning period in both sexes was from November to March. In addition, the occurrence of blood cockle larvae peaked in November and March. Furthermore, a comparison of the distribution between umbo-stage and full-grown larvae suggested the umbo-stage larvae diffused offshore and return to coastal sites where they settle and mature into full-grown larvae

Food availability estimation of the blood cockle, Anadara granosa (Linnaeus, 1758), from the aquaculture grounds of the Selangor Coast, Malaysia

Blood cockles, Anadara granosa (Linnaeus, 1758), were collected from the aquaculture grounds (4 stations) of the Selangor coast, Malaysia, and the water quality (water temperature, salinity, dissolved oxygen, turbidity, and chlorophyll a) was measured from September 2011 to June 2013. At all stations, the water temperature fluctuated around 30℃. At station C, located at the mouth of the Selangor River, the salinity was occasionally lower than 20 PSU. However, the salinity of the other stations fluctuated around 30 PSU. In addition, at all stations, the content of dissolved oxygen generally fluctuated around 3 mg.L-1 or above, and the turbidity changed irregularly, sometimes exceeding 300 Forumajin Turbidity Units. The chlorophyll a content fluctuated mainly ranging 4-20 µg.L-1 at all stations, and values above 20 µg.L-1 were occasionally observed. The phytopigment content, a food availability indicator, in the digestive gland tissue of the blood cockles collected from all stations fluctuated ranging 30-770 µg.g-1. However, there was no proportional correlation between phytopigment content in the digestive gland and chlorophyll a content at all stations. Therefore, even in a high chlorophyll a content (over 20 µg.L-1) environment, the accumulated phytopigment in the digestive gland was around 290 µg.g-1. In general, these results indicated the cockles were eating a sufficient amount of foods (organic materials including phytoplankton) all year round during the study period. And, the food availability environment in the aquaculture grounds of the Selangor coast was estimated sufficient to grow the blood cockle

Efficient spawning conditions of kuruma prawn Marsupenaeus japonicus to induce maturation

Text in Japanese with English abstract.Efficient spawning conditions through artificially induced maturation by unilateral eyestalk ablation were investigated for the kuruma prawn Marsupenaeus japonicus. After unilateral eyestalk ablation, 24℃ was the most efficient water temperature for spawning (rate, number of spawning times, and number of eggs) in the comparison of four water temperatures (20, 22, 24, and 26℃). Egg diameter tended to increase with decreasing water temperature. As a result of unilateral eyestalk ablation at different molting stages, the postmolt stage was better in spawning rate and survival until spawning. Based on these results, individuals that seemed to be in the postmolt stage were selected manually, subjected to eyestalk ablation, and reared at 24℃. Prawns were randomly split into two feeding groups of compound feed or live polychaete. Unilateral eyestalk ablation enabled spawning of the group consuming the compound feed. For both feeding groups, most individuals spawned on Day 10

Permanent genetic resources added to molecular ecology resources database 1 june 2011–31 july 2011

3 pagesInternational audienceThis article documents the addition of 112 microsatellite marker loci and 24 pairs of single nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Agelaius phoeniceus, Austrolittorina cincta, Circus cyaneus, Circus macrourus, Circus pygargus, Cryptocoryne × purpurea Ridl. nothovar. purpurea, Mya arenaria, Patagioenas squamosa, Prochilodus mariae, Scylla serrata and Scytalopus speluncae. These loci were cross-tested on the following species: Cryptocoryne × purpurea nothovar. purpurea, Cryptocoryne affinis, Cryptocoryne ciliata, Cryptocoryne cordata var. cordata, Cryptocoryne elliptica, Cryptocoryne griffithii, Cryptocoryne minima, Cryptocoryne nurii and Cryptocoryne schulzei. This article also documents the addition of 24 sequencing primer pairs and 24 allele-specific primers or probes for Aphis glycines

Permanent genetic resources note. Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2012-30 September 2012

This article documents the addition of 83 microsatellite marker loci and 96 pairs of single-nucleotide polymorphism (NSP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Bembidion lampros, Inimicus japonicus, Lymnaea stagnalis, Panopea abbreviata, Pentadesma butyracea, Sycoscapte rhirticola and Thanatephorus cucumeris (anamorph: Rhizoctonia solani). These loci were cross-tested on the following species: Pentadesma grandiofolia and Pentadesma reyndersii. This article also documents the addition of 96 sequencing primer pairs and 88 allele-specific primers or probes for Plutella zylostella