Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha

Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, cell morphogenesis and organelle motility. Imaging the actin cytoskeleton in living cells is a powerful methodology for studying these important phenomena. Several useful probes for live imaging of filamentous actin (F-actin) have been developed, but new versatile probes are still needed. Here, we report the application of a new probe called Lifeact for visualizing F-actin in plant cells. Lifeact is a short peptide comprising 17 amino acids that was derived from yeast Abp140p. We used a Lifeact–Venus fusion protein for staining F-actin in Arabidopsis thaliana and were able to observe dynamic rearrangements of the actin meshwork in root hair cells. We also used Lifeact–Venus to visualize the actin cytoskeleton in the liverwort Marchantia polymorpha; this revealed unique and dynamic F-actin motility in liverwort cells. Our results suggest that Lifeact could be a useful tool for studying the actin cytoskeleton in a wide range of plant lineages

AC Loss Properties of Laser-Scribed Multi-Filamentary GdBCO Coated Conductors with Artificial Pinning Centres

AbstractRE1Ba2Cu3O7-∂ (RE: Rare Earth, Gd, Y and so on) coated conductors have a large aspect ratio and also anisotropy in critical current density. For practical application of REBCO tapes, we evaluated Jc and ac loss properties in a wide range of temperature and magnetic field. The induced ac losses due to the perpendicular magnetic field to a tape face are considerably huge. Therefore, in case that REBCO coated conductors are applied electric power machines and devices, ac loss induced in the windings should amount to a great part of the total heat load. We proposed an ac loss reduction method, which is combined with a laser scribing technique and a special winding process into a multi–filamentary structure. Additionally, we are trying to enhance critical current density JC, by doping artificial pinning centre. In this study we investigated the ac loss properties of multi-filament structural GdBCO tapes with BZO nano–lods as artificial pinning centers. We measured ac losses of 5, 10, 20-filament GdBCO tapes by using a saddle-shaped pickup coil. Consequently we confirmed the ac loss decreased in inverse proportion to the number of filaments even in the GdBCO tapes with enhanced JC by introducing artificial pinning centers