Early Permian ammonoids from the Kaeng Krachan Group of the Phatthalung-Hat Yai area, southern peninsular Thailand

An Early Permian small ammonoid fauna consisting of Neocrimites sp., Agathiceras suessi Gemmellaro, A. girtyi Bo¨se, Agathiceras? sp.,and Miklukhoceras sp. was found in nodules of a fine sandstone bed exposed in the Phatthalung-Hat Yai area of southern peninsular Thailand.The ammonoid-bearing bed belongs stratigraphically to the uppermost part of the Kaeng Krachan Group, which is essentially a clasticdominant, Late Carboniferous (?) to Early Permian stratigraphic unit, widely distributed in western and peninsular Thailand. This ammonoidfauna is considered to be of Bolorian (Kungurian) age and includes Agathiceras girtyi Bo¨se, which is described for the first time from Thailand. The present discovery of Bolorian ammonoids suggests that the uppermost part of the Kaeng Krachan Group is slightly younger than previously considered and around the latest Early Permian. This further implies that the continental margin environment of the Sibumasu Block drastically changed at around Bolorian time from a cool, clastic-dominant shelf condition to a temperate to subtropical, carbonate platform due to rapid northward drift after middle Artinskian rifting

Transmission electron microscopic observations of nanobubbles and their capture of impurities in wastewater

Unique properties of micro- and nanobubbles (MNBs), such as a high adsorption of impurities on their surface, are difficult to verify because MNBs are too small to observe directly. We thus used a transmission electron microscope (TEM) with the freeze-fractured replica method to observe oxygen (O2) MNBs in solutions. MNBs in pure water and in 1% NaCl solutions were spherical or oval. Their size distribution estimated from TEM images close to that of the original solution is measured by light-scattered methods. When we applied this technique to the observation of O2 MNBs formed in the wastewater of a sewage plant, we found the characteristic features of spherical MNBs that adsorbed surrounding impurity particles on their surface

Twist expression promotes migration and invasion in hepatocellular carcinoma

Background: Twist, a transcription factor of the basic helix-loop-helix class, is reported to regulate cancer metastasis. It is known to induce epithelial-mesenchymal transition (EMT). In this study, we evaluated the expression of twist and its effect on cell migration in hepatocellular carcinoma (HCC). Methods: We examined twist expression using immunohistochemistry in 20 tissue samples of hepatocellular carcinoma, and assessed twist expression in HCC cell lines by RT-PCR and Western blot analysis. Ectopic twist expression was created by introducing a twist construct in the twist-negative HCC cell lines. Endogenous twist expression was blocked by twist siRNA in the twist-positive HCC cell lines. We studied EMT related markers, E-cadherin, Vimentin, and N-cadherin by Western blot analysis. Cell proliferation was measured by MTT assay, and cell migration was measured by in vitro wound healing assay. We used immunofluorescent vinculin staining to visualize focal adhesion. Results: We detected strong and intermediate twist expression in 7 of 20 tumor samples, and no significant twist expression was found in the tumor-free resection margins. In addition, we detected twist expression in HLE, HLF, and SK-Hep1 cells, but not in PLC/RPF/5, HepG2, and Huh7 cells. Ectopic twist-expressing cells demonstrated enhanced cell motility, but twist expression did not affect cell proliferation. Twist expression induced epithelial-mesenchymal transition together with related morphologic changes. Focal adhesion contact was reduced significantly in ectopic twist-expressing cells. Twist-siRNA-treated HLE, HLF, and SK-Hep1 cells demonstrated a reduction in cell migration by 50, 40 and 18%, respectively. Conclusion: Twist induces migratory effect on hepatocellular carcinoma by causing epithelial-mesenchymal transition

ウェーブレットヘンカン ニヨル ガゾウカイセキ

The digital signal processing techiuques in connection with the development of availability of internet, multi-channel TV broadcast, etc. become important in the 21th century. In this paper, we discuss the characterics and problems on the digital image processing by using the discrete wavelet transform, which has been come into notice with the Fourier transform and/or the cosine transform analysis