Myc Regulates Embryonic Vascular Permeability and Remodeling

Previous work has shown that c-Myc is required for adequate vasculogenesis and angiogenesis. To further investigate the contribution of Myc to these processes, we conditionally expressed c-Myc in embryonic endothelial cells using a tetracycline-regulated system. Endothelial Myc overexpression resulted in severe defects in the embryonic vascular system. Myc-expressing embryos undergo widespread edema formation and multiple hemorrhagic lesions. They die between embryonic days 14.5 and 17.5. The changes in vascular permeability are not caused by deficiencies in vascular basement membrane composition or pericyte coverage. However, the overall turnover of endothelial cells is elevated as is revealed by increased levels of both proliferation and apoptosis. Whole-mount immunohistochemical analysis revealed alterations in the architecture of capillary networks. The dermal vasculature of Myc-expressing embryos is characterized by a reduction in vessel branching, which occurs despite upregulation of the proangiogenic factors vascular endothelial growth factor-A and angiopoietin-2. Thus, the net outcome of an excess of vascular endothelial growth factor-A and angiopoietin-2 in the face of an elevated cellular turnover appears to be a defect in vascular integrity. (Circ Res. 2009;104:1151-1159.

Navigating cyber resilience in seaports: challenges of preparing for cyberattacks at the Port of Rotterdam

Purpose Cyber resilience has emerged as an approach for seaports to deal with cyberattacks; it emphasizes ports’ ability to prepare for an attack and to keep operating and recover quickly. However, little research has been undertaken on the challenges of governing cyber risks in seaports. This study aims to address this gap. Design/methodology/approach Governing cyber resilience is shaped by distributed responsibilities, uncertainties and ambiguities. The authors use this conceptualization to explore the governance of cyber risks in seaports, taking the Port of Rotterdam as a case study and analyzing semistructured interviews with stakeholders, participatory observation and policy documents and legislation. Findings The authors found that many strategies for governing cyber risks remain dedicated to protecting computer systems against cyberattacks. Nevertheless, port stakeholders have also developed strategies in anticipation of disruptions. However, these strategies appear informal and uncoordinated due to a lack of information exchange, insufficient knowledge regarding cyber risks and disagreement about how to make the Port of Rotterdam cyber resilient. What mainly hampers the cyber resilience of the port is the lack of a comprehensive regulatory framework and economic incentives. The authors conclude that resilience is merely an ideal at the Port of Rotterdam, meaning related governance strategies remain incremental and await institutionalization. Originality/value This paper offers insights into the cyber resilience of critical socio-technical systems, which have been underexposed in cyber resilience debates, but, when exploited, can manifest in large-scale disruptions

Beyond the dikes: an institutional perspective on governing flood resilience at the Port of Rotterdam

Seaports, infrastructural nodes in global supply chains and production processes, are vulnerable to flood risks: they are crisis-prone critical infrastructure (CI) systems. However, the governance of their flood resilience involves many different private and public actors in a complex institutional environment and there is no scholarly consensus about how resilience can be successfully governed. We investigate the governance of flood resilience at the Port of Rotterdam (PoR) from an institutional perspective, by studying institutional arrangements for flood resilience within and across vertical, horizontal and territorial dimensions to elucidate the strengths and ongoing challenges of shaping the port’s flood resilience. We conducted semi-structured expert interviews (n = 17) and an analysis of policy documents and legislation (n = 33) relating to flood risk management and CI protection. We find that the institutional design for flood resilience in the Netherlands consists of a complex matrix of responsibilities, capacities and plans. While coordination is visible in the shared visions and strategies for flood resilience developed at different policy levels and domains, we find fragmentation and persisting institutional challenges, including siloed governance approaches, knowledge gaps and blurred distribution of responsibilities; these are significant barriers to enhancing flood resilience for CIs and port–city relationships

Regulation of mRNA translation by MID1: a common mechanism of expanded CAG repeat RNAs

Expansion of CAG repeats, which code for the disease-causing polyglutamine protein, is a common feature in polyglutamine diseases. RNA-mediated mechanisms that contribute to neuropathology in polyglutamine diseases are important. RNA-toxicity describes a phenomenon by which the mutant CAG repeat RNA recruits RNA-binding proteins, thereby leading to aberrant function. For example the MID1 protein binds to mutant huntingtin (HTT) RNA, which is linked to Huntington's disease (HD), at its CAG repeat region and induces protein synthesis of mutant protein. But is this mechanism specific to HD or is it a common mechanism in CAG repeat expansion disorders? To answer this question, we have analyzed the interaction between MID1 and three other CAG repeat mRNAs, Ataxin2 (ATXN2), Ataxin3 (ATXN3), and Ataxin7 (ATXN7), that all differ in the sequence flanking the CAG repeat. We show that ATXN2, ATXN3, and ATXN7 bind to MID1 in a CAG repeat length-dependent manner. Furthermore, we show that functionally, in line with what we have previously observed for HTT, the binding of MID1 to ATXN2, ATXN3, and ATXN7 mRNA induces protein synthesis in a repeat length-dependent manner. Our data suggest that regulation of protein translation by the MID1 complex is a common mechanism for CAG repeat containing mRNAs

Die Nutzung von Bodenschätzungsdaten zur Modellierung von landwirtschaftlicher Biomasse

Mittels des Biomasse-Ertragsmodells bym können regionale, landwirtschaftliche Standortpotenziale berechnet werden. Grundlage der Modellierung bilden Geodaten, deren Qualität und Verfügbarkeit das Ergebnis beeinflussen. Ein wesentlicher Parameter ist die Ertragsfähigkeit der Böden

Urban Infrastructures: Criticality, Vulnerability and Protection. Report of the International Conference of the Research Training Group KRITIS at Technische Universität Darmstadt, Germany

From the 7th to the 8th of February 2019, more than 70 scientists from different disciplines and countries came together for the international Conference “Urban Infrastructure: Criticality, Vulnerability and Protection” which was organised by the Research Training Group KRITIS at Technische Universität Darmstadt. The focus of the conference was on networked critical infrastructures (CI) in cities as socio-technical systems that require special protection strategies due to their vulnerabilities. Five multidisciplinary panels on Governance, Spatiality, Temporality, Safety and Security, and ICT Solutions elucidated urban CI protection. The keynote lectures by Per Högselius (KTH Royal Institute of Technology, Stockholm), Jon Coaffee (University of Warwick; New York University) and Christoph Lamers (State Fire Service Institute North Rhine Westfalia) highlighted and deepened the aspects relevant to this context. Despite all the diversity of the contributions from many different disciplines, one aspect has always been prominent: the enormous complexity of urban CI. Regardless of the task at hand - governing and planning cities, creating security concepts and making cities more resilient - the complexity of the CI systems must always be considered. On the conference, civil engineers, computer scientists, urban and spatial planners, architects, sociologists, political scientists, historians and philosophers as well as practitioners from public administration, and operators of critical infrastructures made interesting suggestions on how to deal with the uncertainties involved. It also became clear that current challenges require approaches that cannot be found in a single discipline alone

Unsettled Urban Space: Routines, Temporalities and Contestations

While urban life can be characterized by endeavors to settle stable and safe environments, for many people, urban space is rarely stable or safe; it is uncertain, troubled, imbued with challenges and perpetually under pressure. As the concept of unsettled appears to define the contemporary urban experience, this multidisciplinary book investigates the conflicts and possibilities of settling and unsettling through open and speculative analysis. The analytical prism of unsettled renders urban space an indeterminate ground unfolding through routines, temporalities and contestations in constant tension between settling and unsettling. Such contrasting experiences are contingent on how urban societies confront, undergo and overcome turbulence and difficulties in time and space. Contributions drawing on theoretical reflections and empirical accounts—from Argentina, Austria, Germany, Greece, Italy, the Netherlands, the UAE, the UK, the USA and Vietnam—give insights into plural occurrences of the unsettled, which might tie down or unleash transformative, liberatory and emancipatory potentials. This book is for students, professionals and researchers interested in the uncertainties, foundations, disturbances, inconsistencies, residuals and blind fields, which constitute the urban both as lived space and as social, cultural and political ideal

Cross-Sectional Study on Influenza Vaccination, Germany, 1999–2000

To assess influenza vaccination coverage in Germany, we conducted a nationwide telephone survey in November 1999 in adults (>18 yrs) using random-digit dialing. Overall, 23% of 1,190 survey participants reported having been vaccinated (adjusted 18%) with 16% (adjusted 15%) in former West Germany versus 35% (adjusted 32%) in former East Germany. Immunization rates for vaccination target groups were lower in West Germany (21%) than in East Germany (40%). Seven percent of health-care workers were immunized. Previous influenza vaccination, positive attitudes towards immunization, and having a family physician increased the rate of vaccination; fear of adverse effects lowered the rate. Family physicians performed 93% of the vaccinations, which suggests their key role in improving low vaccination coverage in Germany. The fact that >71% (850/1,190) of participants belonged to at least one of the vaccination target groups recommended by the German Standing Commission on Immunization emphasizes the need to focus the definition of target groups

Bewertung der Ökologie, Wirtschaftlichkeit und sozialen Effekte von ressourceneffizienzsteigernden Maßnahmen auf Quartiersebene – von der volkswirtschaftlichen zur akteursspezifischen Perspektive (NaMaRes): Schlussbericht des Forschungsvorhabens

Das NaMaRes-Projekt entwickelte Werkzeuge und Handlungsempfehlungen zur Unterstützung einer nachhaltigen und ressourceneffizienten Stadtplanung auf Quartiersebene. Die betrachteten Ressourcen umfassen Wasser, Stoffe, Fläche und Ökosystemleistungen. Die Projektergebnisse verbessern die Informationslage über Ressourcen in Bestandsquartieren und bieten eine Grundlage für Akteure in politischen Diskussionen und städtischer Umsetzungsplanung. Das Projekt trägt zur Umsetzung der SDGs in Städten bei