Analyses of Victorian hog deer (axis porcinus) checking station data: demographics, body condition and time of harvest

This report looks into the sustainability and health of deer within Victoria\u27s regional areas. Hog Deer (Axis porcinus) are a popular and highly valued game species in Victoria, with licensed hunters permitted to harvest one male and one female during an annual hunting season during the month of April. All harvested deer must be tagged and presented at a checking station within 24 hours of harvest. A variety of morphological and biological data are recorded for each harvested animal during inspection at the checking stations. The objectives of this study were to (i) summarise biological data collected for all Hog Deer inspected at the four mainland checking stations during 1997–2011 (i.e. excluding Sunday Island, which is owned and managed by the Para Park Co-operative Game Reserve Limited), and (ii) provide recommendations for improving the usefulness of future data collection. A total of 1122 deer were presented at the mainland checking stations (70.4% male; 29.6% female) during 1997–2011, with annual totals ranging from 38 in 1999 to 111 in 2011. There was little evidence that the number or sex ratio of deer harvested annually changed substantially over the course of the study period. The overall percentages of deer harvested on public (52%) and private (48%) land also did not show any discernable trend during the study period. The ages of deer (estimated by molar eruption and tooth wear) ranged from 1 to 12 years for females and males. Although the age structures differed slightly for females and males, there was no evidence that this changed over the study period, although inconsistent recording of ages limited the opportunity for quantitative analyses of these data

A Pilot Study of a Modified Acceptance and Commitment Therapy Smoking Cessation Treatment for Veterans with PTSD

Veterans with posttraumatic stress disorder (PTSD) have high rates of smoking and significant difficulties with quitting. Acceptance and mindfulness-based techniques may enhance smoking cessation approaches for veterans with PTSD as they are designed to improve emotion regulation skills related to coping with elevated negative affect and withdrawal symptoms associated with quit attempts. Veterans with current PTSD and smoking ≥ 15 cigarettes/day (N=19) participated in an open trial of Acceptance and Commitment Therapy for Veterans with PTSD and Tobacco Use (ACT-PT). Participants attended nine weekly individual counseling sessions and received eight weeks of the nicotine patch. Primary outcomes included expired-air carbon monoxide confirmed seven-day point prevalence abstinence, number of cigarettes/day at the end of treatment, and PTSD symptoms on the PTSD Checklist (PCL). Intent-to-treat analyses examined pre-treatment to post-treatment scores on the PCL. At the end of treatment (one month after targeted quit date), 37% (7/19) of participants were abstinent from smoking, 37% (7/19) were abstinent from smoking at the one month follow-up, and 16% (3/19) were abstinent at the three month follow-up. Subjects reduced from 26 cigarettes/day at baseline to 10 cigarettes/day at the end of treatment (p\u3c.001), and 15 cigarettes/day at the 3-month follow-up (p=.002). PTSD symptoms significantly decreased from baseline to the end of treatment (p\u3c.001), and continued to remain significantly decreased at the 3-month follow-up (p=.011). The retention rate (74%), client satisfaction ratings and qualitative feedback from subjects indicated that the treatment was acceptable. Although preliminary, these results suggest that ACT-PT is a promising smoking cessation treatment for veterans with PTSD. Longer follow-up and randomized controlled studies are needed

Reliable Discrimination of 10 Ungulate Species Using High Resolution Melting Analysis of Faecal DNA

Identifying species occupying an area is essential for many ecological and conservation studies. Faecal DNA is a potentially powerful method for identifying cryptic mammalian species. In New Zealand, 10 species of ungulate (Order: Artiodactyla) have established wild populations and are managed as pests because of their impacts on native ecosystems. However, identifying the ungulate species present within a management area based on pellet morphology is unreliable. We present a method that enables reliable identification of 10 ungulate species (red deer, sika deer, rusa deer, fallow deer, sambar deer, white-tailed deer, Himalayan tahr, Alpine chamois, feral sheep, and feral goat) from swabs of faecal pellets. A high resolution melting (HRM) assay, targeting a fragment of the 12S rRNA gene, was developed. Species-specific primers were designed and combined in a multiplex PCR resulting in fragments of different length and therefore different melting behaviour for each species. The method was developed using tissue from each of the 10 species, and was validated in blind trials. Our protocol enabled species to be determined for 94% of faecal pellet swabs collected during routine monitoring by the New Zealand Department of Conservation. Our HRM method enables high-throughput and cost-effective species identification from low DNA template samples, and could readily be adapted to discriminate other mammalian species from faecal DNA

Ionic liquids and organic ionic plastic crystals utilizing small phosphonium cations

The development of new liquid and solid state electrolytes is paramount for the advancement of electrochemical devices such as lithium batteries and solar cells. Ionic liquids have shown great promise in both these applications. Here we demonstrate the use of phosphonium cations with small alkyl chain substituents, in combination with a range of different anions, to produce a variety of new halide free ionic liquids that are fluid, conductive and with sufficient thermal stability for a range of electrochemical applications. Walden plot analysis of the new phosphonium ionic liquids shows that these can be classed as &quot;good&quot; ionic liquids, with low degrees of ion pairing and/or aggregation, and the lithium deposition and stripping from one of these ionic liquids has been demonstrated. Furthermore, for the first time phosphonium cations have been used to form a range of organic ionic plastic crystals. These materials can show significant ionic conductivity in the solid state and thus are of great interest as potential solid-state electrolyte materials. <br /

Origins and population genetics of sambar deer (Cervus unicolor) introduced to Australia and New Zealand

Context. Some populations of introduced species cause significant undesirable impacts but can also act as reservoirs for genetic diversity. Sambar deer (Cervus unicolor) are ‘Vulnerable’ in their native range and invasive in Australia and New Zealand. Genetic data can be used to determine whether these introduced populations might serve as genetic reservoirs for declining native populations and to identify spatial units for management. Aims. We aimed to identify the provenance of sambar deer in Australia and New Zealand, and to characterise their genetic diversity and population structure. Methods. We used mitochondrial control region sequences and 18 nuclear microsatellite loci of 24 New Zealand and 63 Australian sambar deer collected across continuous habitat in each location. We estimated genetic diversity and population differentiation by using pairwise FST, AMOVA, and STRUCTURE analyses. We compared our data with 27 previously published native and invasive range sequences to identify phylogenetic relationships. Key results. Sambar deer in Australia and New Zealand are genetically more similar to those in the west of the native range (South and Central Highlands of India, and Sri Lanka), than to those in the east (eastern India, and throughout Southeast Asia). Nuclear genetic diversity was lower than in the native range; only one mitochondrial haplotype was found in each introduced population. Australian and New Zealand sambar deer were genetically distinct but there was no population structure within either population. Conclusions. The genetic differences we identified between these two introduced populations at putatively neutral loci indicate that there also may be underlying diversity at functional loci. The lack of population genetic structure that we found within introduced populations suggests that individuals within these popula- tions do not experience barriers to dispersal across the areas sampled. Implications. Although genetic diversity is reduced in the introduced range compared with the native range, sambar deer in Australia and New Zealand harbour unique genetic variants that could be used to strengthen genetic diversity in populations under threat in the native range. The apparent high levels of gene flow across the areas we sampled suggest that localised control is unlikely to be effective in Australia and New Zealand

Collaborative research and development (R&D) for climate technology transfer and uptake in developing countries: Towards a needs driven approach

While international cooperation to facilitate the transfer and uptake of climate technologies in developing countries is an ongoing part of climate policy conversations, international collaborative R&D has received comparatively little attention. Collaborative R&D, however, could be a potentially important contributor to facilitating the transfer and uptake of climate technologies in developing countries. But the complexities of international collaborative R&D options and their distributional consequences have been given little attention to date. This paper develops a systematic approach to informing future empirical research and policy analysis on this topic. Building on insights from relevant literature and analysis of empirical data based on a sample of existing international climate technology R&D initiatives, three contributions are made. First, the paper analyses the coverage of existing collaborative R&D efforts in relation to climate technologies, highlighting some important concerns, such as a lack of coverage of lower-income countries or adaptation technologies. Second, it provides a starting point for further systematic research and policy thinking via the development of a taxonomic approach for analysing collaborative designs. Finally, it matches characteristics of R&D collaborations against developing countries’ climate technology needs to provide policymakers with guidance on how to Configure R&D collaborations to meet these needs

Genetic analysis reveals spatial structure in an expanding introduced rusa deer population

Context. Rusa deer (Cervus timorensis), originally introduced in the 1860s, are still spreading in eastern Australia. The expanding peri-urban rusa deer population in the Illawarra region of New South Wales, Australia is having undesirable impacts on human and ecological communities, but the spatial structure of this population has not been investigated. Genetic information on invasive species is potentially useful in identifying management units to mitigate undesirable impacts. Aims. The aim of this study was to investigate population structure, characterise dispersal, and determine if natural and human-made landscape features affected gene flow in rusa deer invading the Illawarra region of New South Wales. Methods. We used reduced representation sequencing (DArT-Seq) to analyse single nucleotide polymorphisms distributed throughout the genomic DNA of rusa deer culled during a management program. We used admixture and Principal Component Analyses to investigate population structure with respect to natural and human-made landscape features, and we investigated whether our genetic data supported the presence of sex-biased dispersal. Key results. Genetic diversity was highest in the north, near the original introduction site. A railway line demarcated restricted gene flow. Surprisingly, the Illawarra escarpment, a prominent landscape feature, did not restrict gene flow. There was no evidence of sex-biased dispersal and seven individuals were identified as genetic outliers. Conclusions. The genetic structure of the Illawarra rusa deer population is consistent with individuals spreading south from their introduction site in Royal National Park. The population is not panmictic, and a landscape feature associated with urbanisation was associated with increased spatial genetic structure. Outliers could indicate hybridisation or secondary incursion events. Implications. Rusa deer can be expected to continue invading southwards in the Illawarra region, but landscape features associated with urbanisation might reduce dispersal across the landscape. The genetic structuring of the population identified three potential management units on which to prioritise ground shooting operations

Serosurveillance and Molecular Investigation of Wild Deer in Australia Reveals Seroprevalence of Pestivirus Infection

Since deer were introduced into Australia in the mid-1800s, their wild populations have increased in size and distribution, posing a potential risk to the livestock industry, through their role in pathogen transmission cycles. In comparison to livestock, there are limited data on viral infections in all wildlife, including deer. The aim of this study was to assess blood samples from wild Australian deer for serological evidence of exposure to relevant viral livestock diseases. Blood samples collected across eastern Australia were tested by ELISA to detect antigens and antibodies against Pestivirus and antibodies against bovine herpesvirus 1. A subset of samples was also assessed by RT-PCR for Pestivirus, Simbu serogroup, epizootic hemorrhagic disease virus and bovine ephemeral fever virus. Our findings demonstrated a very low seroprevalence (3%) for ruminant Pestivirus, and none of the other viruses tested were detected. These results suggest that wild deer may currently be an incidental spill-over host (rather than a reservoir host) for Pestivirus. However, deer could be a future source of viral infections for domestic animals in Australia. Further investigations are needed to monitor pathogen activity and quantify possible future infectious disease impacts of wild deer on the Australian livestock industry