Impacts of in vivo and in vitro exposures to tamoxifen: comparative effects on human cells and marine organisms

Tamoxifen (TAM) is a first generation-SERM administered for hormone receptor-positive (HER+) breast cancer in both pre- and post-menopausal patients and may undergo metabolic activation in organisms that share similar receptors and thus face comparable mechanisms of response. The present study aimed to assess whether environmental trace concentrations of TAM are bioavailable to the filter feeder M. galloprovincialis (100 ng L-1) and to the deposit feeder N. diversicolor (0.5, 10, 25 and 100 ng L-1) after 14 days of exposure. Behavioural impairment (burrowing kinetic), neurotoxicity (AChE activity), endocrine disruption by alkali-labile phosphate (ALP) content, oxidative stress (SOD, CAT, GPXs activities), biotransformation (GST activity), oxidative damage (LPO) and genotoxicity (DNA damage) were assessed. Moreover, this study also pertained to compare TAM cytotoxicity effects to mussels and targeted human (i.e. immortalized retinal pigment epithelium - RPE; and human transformed endothelial cells - HeLa) cell lines, in a range of concentrations from 0.5 ng L-1 to 50 μg L-1. In polychaetes N. diversicolor, TAM exerted remarkable oxidative stress and damage at the lowest concentration (0.5 ng L-1), whereas significant genotoxicity was reported at the highest exposure level (100 ng L-1). In mussels M. galloprovincialis, 100 ng L-1 TAM caused endocrine disruption in males, neurotoxicity, and an induction in GST activity and LPO byproducts in gills, corroborating in genotoxicity over the exposure days. Although cytotoxicity assays conducted with mussel haemocytes following in vivo exposure was not effective, in vitro exposure showed to be a feasible alternative, with comparable sensitivity to human cell line (HeLa).info:eu-repo/semantics/publishedVersio

The Evolution of Helium and Hydrogen Ionization Corrections as HII Regions Age

Helium and hydrogen recombination lines observed in low-metallicity, extragalactic, HII regions provide the data used to infer the primordial helium mass fraction, Y_P. In deriving abundances from observations, the correction for unseen neutral helium or hydrogen is usually assumed to be absent; i.e., the ionization correction factor is taken to be unity (icf = 1). In a previous paper (VGS), we revisited the question of the icf, confirming a "reverse" ionization correction: icf < 1. In VGS the icf was calculated using more nearly realistic models of inhomogeneous HII regions, suggesting that the published values of Y_P needed to be reduced by an amount of order 0.003. As star clusters age, their stellar spectra evolve and so, too, will their icfs. Here the evolution of the icf is studied, along with that of two, alternate, measures of the "hardness" of the radiation spectrum. The differences between the icf for radiation-bounded and matter-bounded models are also explored, along with the effect on the icf of the He/H ratio (since He and H compete for some of the same ionizing photons). Particular attention is paid to the amount of doubly-ionized helium predicted, leading us to suggest that observations of, or bounds to, He++ may help to discriminate among models of HII regions ionized by starbursts of different ages and spectra. We apply our analysis to the Izotov & Thuan (IT) data set utilizing the radiation softness parameter, the [OIII]/[OI] ratio, and the presence or absence of He++ to find 0.95 < icf < 0.99. This suggests that the IT estimate of the primordial helium abundance should be reduced by Delta-Y = 0.006 +- 0.002, from 0.244 +- 0.002 to 0.238 +- 0.003.Comment: 27 double-spaced pages, 11 figures, 5 equations; revised to match the version accepted for publication in the Ap

Avaliação dos impactos ambientais e sociais da tecnologia "Capim elefante pioneiro no sistema de pastejo rotativo".

Resumo A tecnologia "Capim-elefante pioneiro sob pastejo rotativo" representa uma alternativa para intensificação da produção em pequenas propriedades, favorecendo a geração de renda e a segurança alimentar das famílias. O objetivo deste trabalho foi avaliar os impactos sociais e ambientais dessa tecnologia, por meio da aplicação do Sistema AMBITEC, desenvolvido pela Embrapa Meio Ambiente. Foram feitas entrevistas individuais com os proprietários rurais, e inserção dos dados levantados nas planilhas eletrônicas para geração dos índices de impacto. Os impactos sociais não foram muito significativos, embora positivos para os itens avaliados. Ocorreram melhorias em relação à oportunidade de emprego qualificado e capacitação, segurança alimentar e gestão e administração. A magnitude do impacto ambiental foi pouco expressiva, entretanto positivo. Os indicadores de impacto que melhor reagiram à tecnologia foram o uso de recursos naturais, água e capacidade produtiva do solo

Parsec-Scale Images of Flat-Spectrum Radio Sources in Seyfert Galaxies

We present high angular resolution (~2 mas) radio continuum observations of five Seyfert galaxies with flat-spectrum radio nuclei, using the VLBA at 8.4 GHz. The goal of the project is to test whether these flat-spectrum cores represent thermal emission from the accretion disk, as inferred previously by Gallimore et al. for NGC 1068, or non-thermal, synchrotron self-absorbed emission, which is believed to be responsible for more powerful, flat-spectrum nuclear sources in radio galaxies and quasars. In four sources (T0109-383, NGC 2110, NGC 5252, Mrk 926), the nuclear source is detected but unresolved by the VLBA, indicating brightness temperatures in excess of 10^8 K and sizes, on average, less than 1 pc. We argue that the radio emission is non-thermal and synchrotron self-absorbed in these galaxies, but Doppler boosting by relativistic outflows is not required. Synchrotron self-absorption brightness temperatures suggest intrinsic source sizes smaller than ~0.05-0.2 pc, for these four galaxies, the smallest of which corresponds to a light-crossing time of ~60 light days or 10^4 gravitational radii for a 10^8 M_sun black hole. We also present MERLIN and VLA observations of NGC 4388, which was undetected by the VLBA, and argue that the observed, flat-spectrum, nuclear radio emission in this galaxy represents optically thin, free-free radiation from dense thermal gas on scales ~0.4 to a few pc. It is notable that the two Seyfert galaxies with detected thermal nuclear radio emission (NGC 1068 and NGC 4388) both have large X-ray absorbing columns, suggesting that columns in excess of \~10^{24} cm^{-2} are needed for such disks to be detectable. (Abridged)Comment: 36 pages including 5 tables and 4 figures; accepted for publication in Ap

Can group-based strategies increase community resilience? Longitudinal predictors of sustained participation in Covid-19 mutual aid and community support groups

Mutual aid groups have been a critical part of the coronavirus disease-2019 (Covid-19) response and continue to address the needs of people in their communities. To understand how mutual aid and similar community support groups can be sustained over time, we test the idea that using group-based strategies initiates psychological trajectories that shape future participation. We conducted a preregistered longitudinal survey among Covid-19 mutual aid and community support volunteers in the United Kingdom (nWave 1 = 600, May 2021; nWave 2 = 299, July–August 2021) who were registered panelists of an independent research organization. Assessments included measures of group-based strategies, collective participation predictors, participation experience, and sustained participation. Volunteers engaged in a wide range of support activities including shopping, emotional support provision, and deliveries. Two group-based strategies—group alliances and group horizontality—longitudinally predicted sustained participation. In addition, sense of community responsibility and burnout were longitudinal predictors of sustained participation. Importantly, predictors of sustained participation diverged for volunteers with different levels of volunteering experience. Our findings highlight group-based strategies as a potential resource for organizers seeking to sustain participation. Use can be tailored depending on the profiles of individual Covid-19 mutual aid volunteers. These findings have significance beyond Covid-19 as they are relevant to sustaining community resilience more generally