Intervista ad Annick Bizouerne

intervista ad Annick Bizouerne capo progetto del progetto urbano di Rive Gauche a Parigi.Interview with Annick Bizouerne Project Manager for the urban project Paris Rive Gauche

Muc5ac mucin expression during rat skin development

Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during prenatal and postnatal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.Conclusion: Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.Fil: Ferretti, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Segal Eiras, Amada. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaFil: Croce, María Virginia. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentin

An Overview of Vanadium and Cell Signaling in Potential Cancer Treatments

Vanadium is an ultratrace element present in higher plants, animals, algae, and bacteria. In recent years, vanadium complexes have been studied to be considered as a representative of a new class of nonplatinum metal anticancer drugs. Nevertheless, the study of cell signaling pathways related to vanadium compounds has scarcely been reported on and reviewed thus far; this information is highly critical for identifying novel targets that play a key role in the anticancer activity of these compounds. Here, we perform a review of the activity of vanadium compounds over cell signaling pathways on cancer cells and of the underlying mechanisms, thereby providing insight into the role of these proteins as potential new molecular targets of vanadium complexes.Fil: Ferretti, Valeria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentin

Soil and water as resources: how landscape architecture reclaims hydric contaminated soil for public uses in urban settlements

Soil is one of the fundamental components for life on Earth, but today, as a consequence of humans’ unsustainable actions, soil is polluted, distressed and spoiled. In contemporary practice design, we recognize the importance of the soil quality to structure new discourses in landscape practice. The central role in this process is undoubtedly played by the value a healthy soil has for the community and for the environment. The strategic design of wet and hydric landscapes is certainly an essential aspect for the regular and exceptional management of the effects produced by pollution and climate change. The research develops the soil as a key subject in the landscape design, specifically in hydric environments where water represents an important factor. The essay is divided into three parts: resources and opportunities of disturbed wet soil, successfully built public space where soil remediation transformed heavy polluted industrial urban sites in fertile public ecosystems within the dense urban structures, and soil design as a domain of urban resilience. The landscape project as an integrated project has spread the seeds of a new approach to the consideration of the contemporary city in an ecological manner

Soil and Water as Resources: How Landscape Architecture Reclaims Hydric Contaminated Soil for Public Uses in Urban Settlements

Soil is one of the fundamental components for life on Earth, but today, as a consequence of humans’ unsustainable actions, soil is polluted, distressed and spoiled. In contemporary practice design, we recognize the importance of the soil quality to structure new discourses in landscape practice. The central role in this process is undoubtedly played by the value a healthy soil has for the community and for the environment. The strategic design of wet and hydric landscapes is certainly an essential aspect for the regular and exceptional management of the effects produced by pollution and climate change. The research develops the soil as a key subject in the landscape design, specifically in hydric environments where water represents an important factor. The essay is divided into three parts: resources and opportunities of disturbed wet soil, successfully built public space where soil remediation transformed heavy polluted industrial urban sites in fertile public ecosystems within the dense urban structures, and soil design as a domain of urban resilience. The landscape project as an integrated project has spread the seeds of a new approach to the consideration of the contemporary city in an ecological manner

Estudio de la expresión de mucinas epiteliales de carbohidratos asociados y de Romboide 2 (Rhbdd2) en ratas adultas y embrionarias

Tesis presentada para optar al Grado de Doctor en Ciencias NaturalesFil: Ferretti, Valeria Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentin

Synthesis, structure and characterization of a new cadmium(II) iodide containing a tetradentate tripodal amine

A cadmium(II) iodide compound of the [Cd(L)I]I series, with L = tris(2-aminoethyl)amine, is synthesized and characterized. X-ray structural study shows that the title compound has a slightly distorted trigonal bipyramidal environment where the CdN4I chromophore is bounded by N atoms of L and one iodide. In the structural arrangement, the [Cd(L)I]+ cationic complexes are interconnected via N-H···I hydrogen bonds generated by the uncoordinated I iodide anions to form layers parallel to the (a, c) plane. The 13C CP-MAS NMR spectrum is discussed and the vibrational absorption bands were identified by infrared spectroscopy and DFT calculations

Resolvin D1 Halts Remote Neuroinflammation and Improves Functional Recovery after Focal Brain Damage Via ALX/FPR2 Receptor-Regulated MicroRNAs

Remote damage is a secondary phenomenon that usually occurs after a primary brain damage in regions that are distant, yet functionally connected, and that is critical for determining the outcomes of several CNS pathologies, including traumatic brain and spinal cord injuries. The understanding of remote damage-associated mechanisms has been mostly achieved in several models of focal brain injury such as the hemicerebellectomy (HCb) experimental paradigm, which helped to identify the involvement of many key players, such as inflammation, oxidative stress, apoptosis and autophagy. Currently, few interventions have been shown to successfully limit the progression of secondary damage events and there is still an unmet need for new therapeutic options. Given the emergence of the novel concept of resolution of inflammation, mediated by the newly identified ω3-derived specialized pro-resolving lipid mediators, such as resolvins, we reported a reduced ability of HCb-injured animals to produce resolvin D1 (RvD1) and an increased expression of its target receptor ALX/FPR2 in remote brain regions. The in vivo administration of RvD1 promoted functional recovery and neuroprotection by reducing the activation of Iba-1+ microglia and GFAP+ astrocytes as well as by impairing inflammatory-induced neuronal cell death in remote regions. These effects were counteracted by intracerebroventricular neutralization of ALX/FPR2, whose activation by RvD1 also down-regulated miR-146b and miR-219a-1-dependent inflammatory markers. In conclusion, we propose that innovative therapies based on RvD1-ALX/ FPR2 axis could be exploited to curtail remote damage and enable neuroprotective effects after acute focal brain damage

Synthesis, structure and characterization of a new noncentrosymmetric Zn(II) complex with the 2-amino-5-chloropyridine ligand (AClPy)

A new noncentrosymmetric Zn(II) complex with the monodentate ligand 2-amino-5-chloropyridine (AClPy), ZnCl2(C5H5ClN2)2, has been prepared at room temperature and characterized by single crystal X-ray diffraction, 13C CP-MAS-NMR and IR spectroscopies. The basic coordination pattern of the AClPy coordinated metal cations is slighly distorted tetrahedral. The crystal structure is characterized by ZnCl2N2 tetrahedra interconnected via N-H···Cl hydrogen bonds generated by the NH2 amino group to form chains extending along the (a-c) direction. The exocyclic N atom is an electron receiving center, which is consistent with features of imino resonance as evidenced by bond lengths and angles. The crystal structure is stabilized by sets of intra and intermolecular hydrogen bonds. The 13C CP-MAS NMR spectrum is discussed and the vibrational absorption bands are identified by infrared spectroscopy and theoretical calculations

Cr6+ adsorption by modified vermiculite

This work aimed at investigating the adsorption of Cr6+ in water by exfoliated vermiculite. The adsorbant tested in this experiment was a vermiculite (from China) which has been subjected to heating at 1000 °C for 1 minute, resulting in an exfoliated vermiculite. Three effects were studied: 1) contact time; 2) initial concentracion of Cr6+; 3) adsorbent mass. Samples were analysed by X Ray Fluorescence (XRF), X Ray Diffraction (XRD) and the solutions with Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to quantify the adsorbed Cr6+ by the vermiculite. Results from XRD diffraction showed a conversion of vermiculite into flogopite after heating at 1000°C for 1 minute because of: 1) high content of potassium, 2) dehydration and 3) structural re-ordering; after the contact of vermiculite with Cr6+, the mineral structure did not change. The adsorption of Cr6+ was studied by Langmuir, Freundlich and Dubinin-Kaganer-Radushkevich (DKR) isotherm models. DKR model, indicative of a cooperative process, described adsorption equilibrium better than the other two models and the maximum adsorption capacity obtained was of 2.81 mol/g. Kinetic was studied using pseudo-first and pseudo-second order kinetic models, with a better description of the process by pseudo-second order model with correlation coefficient almost unitary (R2=0.9984; other kinetic parameters were k2=0.0015 and the absorption initial rate of 0.2x10-8 mg g-1 h-1). The present study demonstrates the effectiveness of modified vermiculite adsorbents for the treatment of hexavalent chromium-contaminated waters and that its adsorption depends on the experimental conditions (such as contact time, initial concentracion of Cr6+ and adsorbent mass)