909 research outputs found
Processamento de amostras provenientes do trato reprodutivo feminino para extração de RNA genĂŽmico do vĂrus da artrite-encefalite caprina e diagnĂłstico molecular por RT-nested PCR.
Este trabalho descreve os procedimentos necessĂĄrios para a coleta e processamento de amostras provenientes ou recuperadas do trato reprodutivo feminino de cabras infectadas pelo CAEV para posterior extração do RNA viral por meio de um mĂ©todo baseado em centrifugação em coluna de sĂlica. A avaliação da presença do RNA nestas amostras Ă© feita diretamente por meio da reação de RT-nested PCR, potencial mĂ©todo de diagnĂłstico molecular da CAE.bitstream/item/31705/1/UMT-Cot-117.pdfMetodologia Cientifica
Campanhas de produção de sementes de arroz e milho em comunidades rurais rondonienses.
Objetivou-se, especificamente, com estas duas campanhas anual e consecutivas, disponibilizar e transferir tecnologias de produção sementes (sem fins comerciais) de arroz e milho em 125 comunidades rurais rondonienses. Procurou-se tambĂ©m demonstrar que, com orientaçÔes tĂ©cnicas adequadas, seria possĂvel aos pequenos produtores rurais, produzirem sementes com qualidade genĂ©tica e tecnolĂłgica para o seu uso e da comunidade rural, impactando positivamente na melhoria da produtividade e da qualidade do produto produzido.bitstream/item/24650/1/doc120-arroz.pd
Intake and digestibility of river buffalo steers (Bubalus bubalis) fed different levels of palm kernel cake: effect of diet neutral detergent fiber, digestible energy, crude protein and extract ether.
Proceeding of World Buffalo Congress, 9., 2010, Buenos Aires
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-Îł production
<p>Abstract</p> <p>Background</p> <p>Interferon-gamma is a key cytokine in the protective responses against intracellular pathogens. A single nucleotide polymorphism (SNP) located in the first intron of the human IFN-γ gene can putatively influence the secretion of cytokine with an impact on infection outcome as demonstrated for tuberculosis and other complex diseases. Our aim was to investigate the putative association of IFNG+874T/A SNP with American tegumentary leishmaniasis (ATL) and also the influence of this SNP in the secretion of IFN-γ <it>in vitro</it>.</p> <p>Methods</p> <p>Brazilian ATL patients (78 cutaneous, CL, and 58 mucosal leishmaniasis, ML) and 609 healthy volunteers were evaluated. The genotype of +874 region in the IFN-γ gene was carried out by Amplification Refractory Mutational System (ARMS-PCR). <it>Leishmania</it>-induced IFN-γ production on peripheral blood mononuclear cell (PBMC) culture supernatants was assessed by ELISA.</p> <p>Results</p> <p>There are no differences between +874T/A SNP frequency in cases and controls or in ML versus CL patients. Cutaneous leishmaniasis cases exhibiting AA genotype produced lower levels of IFN-γ than TA/TT genotypes. In mucosal cases, high and low IFN-γ producers were clearly demonstrated but no differences in the cytokine production was observed among the IFNG +874T or A carriers.</p> <p>Conclusion</p> <p>Our results suggest that +874T/A polymorphism was not associated with either susceptibility or severity to leishmaniasis. Despite this, IFNG +874T/A SNP could be involved in the pathogenesis of leishmaniasis by influencing the amount of cytokine released by CL patients, although it could not prevent disease development. On the other hand, it is possible that in ML cases, other potential polymorphic regulatory genes such as TNF-α and IL-10 are also involved thus interfering with IFN-γ secretion.</p
Reconstruction of inclined air showers detected with the Pierre Auger Observatory
We describe the method devised to reconstruct inclined cosmic-ray air showers
with zenith angles greater than detected with the surface array of
the Pierre Auger Observatory. The measured signals at the ground level are
fitted to muon density distributions predicted with atmospheric cascade models
to obtain the relative shower size as an overall normalization parameter. The
method is evaluated using simulated showers to test its performance. The energy
of the cosmic rays is calibrated using a sub-sample of events reconstructed
with both the fluorescence and surface array techniques. The reconstruction
method described here provides the basis of complementary analyses including an
independent measurement of the energy spectrum of ultra-high energy cosmic rays
using very inclined events collected by the Pierre Auger Observatory.Comment: 27 pages, 19 figures, accepted for publication in Journal of
Cosmology and Astroparticle Physics (JCAP
A search for point sources of EeV photons
Measurements of air showers made using the hybrid technique developed with
the fluorescence and surface detectors of the Pierre Auger Observatory allow a
sensitive search for point sources of EeV photons anywhere in the exposed sky.
A multivariate analysis reduces the background of hadronic cosmic rays. The
search is sensitive to a declination band from -85{\deg} to +20{\deg}, in an
energy range from 10^17.3 eV to 10^18.5 eV. No photon point source has been
detected. An upper limit on the photon flux has been derived for every
direction. The mean value of the energy flux limit that results from this,
assuming a photon spectral index of -2, is 0.06 eV cm^-2 s^-1, and no celestial
direction exceeds 0.25 eV cm^-2 s^-1. These upper limits constrain scenarios in
which EeV cosmic ray protons are emitted by non-transient sources in the
Galaxy.Comment: 28 pages, 10 figures, accepted for publication in The Astrophysical
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