Diet in the Sardinian Bronze Age: models, collagen isotopic data, issues and perspectives

Traditional reconstructions depict Sardinian Bronze Age economy as a mixed one characterized by a remarkable importance of pastoralism, opposed to a more ‘agricultural’ Neolithic. The first few isotopic data, though not unambiguously, seem to indicate quite the opposite compared with earlier times, reflecting possibly a higher dependence on vegetal foods, with possible marginal contributions of aquatic foods and/or C4 plants at one coastal site. However, the importance of ecosystem-wide shifts in interpreting collagen data for comparative purposes is underlined, with specific examples from Sardinia, and so is the rarity of collections including faunal and botanical specimens alongside humans, which would help establish a local isotopic baseline. Efforts to discriminate animal vs. vegetal contributions in Bronze Age diets that are fully based on C3 ecosystems, therefore, remain tentative. Isotopic research in progress on Sardinian diet and climate from the Copper Age up to the Nuragic Late Bronze (2800-1100 BC) is expected to provide a better grasp on temporal and geographic variation.Les reconstitutions alimentaires traditionnelles dépeignent l’économie de l’âge du Bronze en Sardaigne comme une économie mixte caractérisée par une grande importance de l’élevage des moutons, juxtaposée à une période néolithique plus agricole. Les premières données isotopiques, bien que peu nombreuses encore, semblent, d’une façon ambigüe, plutôt indiquer le contraire lorsqu’on les compare aux données des périodes précédentes. Elles montrent une plus grande dépendance des aliments végétaux avec peut-être la contribution marginale d’aliments aquatiques et/ou des plantes issues du milieu côtier. Afin de palier le manque d’études sur les restes fauniques et botaniques et dans le but de réaliser des comparaisons à l’échelle de la Sardaigne, une analyse détaillée des données isotopiques des collagènes a été réalisée pour cerner l’importance de modifications isotopiques de l’écosystème tout entier. Une recherche en cours sur la diète et sur le climat dans la Sardaigne à partir du Néolithique jusqu’à l’âge du Fer (4700-500 avant J.C.) devrait permettre une meilleure interprétation de la variabilité isotopique d’un point de vue chronologique et géographique

La diète à l'âge du Bronze en Sardaigne : modèles, donnés isotopiques du collagène, problèmes et perspectives

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The Downtown Area of Jonestown, Texas.

The objective was to assess Jonestown's value and potential using the Quadruple Net Value metrics.Three projects are focused on the city of Jonestown. The first being a Quadruple Net Value Analysis report generated by undergraduate Land and Property Development students. The second is a public sewer feasibility study to generate creative solutions to septic and environmental conditions by undergraduate capstone Civil Engineering students. Finally, a graduate student will complete a transportation plan for the city to address connectivity and walkability.Texas Target Communitie

Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity.

Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequences. Despite the availability of a large dataset, well represented by both spike 614 variants, not all approaches showed a conclusive signal of positive selection. Population genetic analysis indicates that 614G increases in frequency relative to 614D in a manner consistent with a selective advantage. We do not find any indication that patients infected with the spike 614G variant have higher COVID-19 mortality or clinical severity, but 614G is associated with higher viral load and younger age of patients. Significant differences in growth and size of 614G phylogenetic clusters indicate a need for continued study of this variant

Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity

Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequences. Despite the availability of a large dataset, well represented by both spike 614 variants, not all approaches showed a conclusive signal of positive selection. Population genetic analysis indicates that 614G increases in frequency relative to 614D in a manner consistent with a selective advantage. We do not find any indication that patients infected with the spike 614G variant have higher COVID-19 mortality or clinical severity, but 614G is associated with higher viral load and younger age of patients. Significant differences in growth and size of 614G phylogenetic clusters indicate a need for continued study of this variant

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Californians' Beliefs About Income Inequality

Rising income inequality and wealth in America have attracted substantial public attention in recent years. As evidence has mounted that the gap betwen the affluent and the poor has been increasing, elected officials, scholars, journalists, and even figures from popular culture have discussed the causes and effects of this phenomenon. The focus of the debate has often been the economic trends rather than the views of a broad sampling of Americans.  But public opinion can condition policy responses, and it is important to measure public opinion on economic inequality, its causes, and potential remedies. This IGS Research Brief seeks to understand public opinion in California about this issue. It describes and analyzes the results of an IGS Poll on income inequality, its perceived causes, and the views of Californians regarding possible responses

Californians' Beliefs About Income Inequality

Rising income inequality and wealth in America have attracted substantial public attention in recent years. As evidence has mounted that the gap betwen the affluent and the poor has been increasing, elected officials, scholars, journalists, and even figures from popular culture have discussed the causes and effects of this phenomenon. The focus of the debate has often been the economic trends rather than the views of a broad sampling of Americans.  But public opinion can condition policy responses, and it is important to measure public opinion on economic inequality, its causes, and potential remedies. This IGS Research Brief seeks to understand public opinion in California about this issue. It describes and analyzes the results of an IGS Poll on income inequality, its perceived causes, and the views of Californians regarding possible responses