Multiset proximity spaces

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Optimiranje fizikalno-kemijskih parametara, djelomično pročišćavanje i karakterizacija izvanstanične alkalne lipaze iz novog soja plijesni Curvularia sp. DHE 5

Thirty isolated fungal strains were screened for lipase production using Phenol Red plates, containing tributyrin as lipidic substrate, and a novel fungus identified genetically as Curvularia sp. DHE 5 was found as the most prominent strain. Various agro-industrial substrates were evaluated as inert supports for lipase production in solid-state fermentation. The highest yield of lipase ((83.4±2.2) U/g on dry mass basis) was reported with wheat bran medium after seven days of fermentation at pH=7.0, temperature of 30 °C, 70 % moisture content, inoculum size of 1.27·107 spore/mL and 2 % olive oil as an inducer. Supplementation of the medium with 0.05 % KCl as an ion source further increased lipase production to (88.9±1.2) U/g on dry mass basis. The enzyme was partially purified through ammonium sulphate fractionation (40 %) followed by dialysis, and its optimum pH and temperature were reported at 8.0 and 50 °C, respectively, with remarkable pH and thermal stability.U radu je ispitana proizvodnja lipaze iz 30 izolata plijesni na podlozi s tributirinom pomoću fenolnog crvenila, pri čemu se najviše istaknuo izolat koji je genetički identificiran kao novi soj plijesni Curvularia sp. DHE 5. Ispitana je mogućnost korištenja različitog agroindustrijskog otpada kao inertnih podloga za proizvodnju lipaze fermentacijom na čvrstoj podlozi. Najveći prinos lipaze od (83,4±2,2) jedinica po gramu suhe tvari dobiven je u podlozi s pšeničnim mekinjama nakon sedam dana fermentacije pri pH-vrijednosti od 7,0; temperaturi od 30 °C, 70 %-tnom udjelu vlage, veličini inokuluma od 1,27·107 spora po mililitru i s dodatkom 2 %-tnog maslinovog ulja. Dodatak 0,05 % KCl kao izvora iona dodatno je potaknuo proizvodnju lipaze na 88,9±1,2 jedinica po gramu suhe tvari. Enzim je djelomično pročišćen (40 %) frakcioniranjem pomoću amonijeva sulfata i dijalizom, te se pokazao izuzetno stabilinim, s optimalnom pH-vrijednosti od 8,0 i optimalnom temperaturom od 50 °C

DNA Fingerprinting, Chemical Composition, Antitumor and Antimicrobial Activities of the Essential Oils and Extractives of four Annona Species from Egypt

The leaf essential oils of four members of the Annonaceae grown in Egypt (namely; Annona cherimola, A. squamosa, A. muricata and A. glabra) have been obtained by hydrodistillation and analyzed by GC-MS in order to compare and contrast the volatile chemical compositions of these species.  The essential oils were screened for in-vitro cytotoxic activity against breast cancer (MCF-7), colon cancer (CACO) and liver cancer (HEPG2) cell lines and antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeuroginosa, Aspergillus flavus and Candida albicans. beta-elemene (25.02%), beta-caryophyllene (37.11%), bicycloelemene (23.58%) and and beta-gurjunene (42.49%), were the major constituents of Annona cherimola, A. squamosa, A. muricata and A. glabra, respectively. Ethanol extracts showed highly significant cytotoxic activities (low IC50) much more than results displayed by essential oils on breast (MCF-7), colon (CACO) and liver (HEPG2) carcinoma cell lines. Relative to breast carcinoma cell line (MCF-7), The IC50 values of ethanol extracts were 3.43, 3.89 and 4.34 ?g/ml for A. cherimola, A. squamosa and A. muricata ethanol extractives respectively. While colon carcinoma cell line (CACO) displayed IC50 values 2.82, 2.97, 3.58 and 3.89 ?g/ml for A. muricata, A. cherimola, A. glabra and A. squamosa, respectively. Liver carcinoma cell line (HEPG2) exhibited IC50 valus of 3.12, 3.43 and 3.73 for A. squamosa, A. muricata and A. cherimola, respectively. Three of the four leaf essential oils showed notable invitro cytotoxic activity. Essential oils of A. glabra, A. muricata and A. squamosa showed moderate cytotoxic activities with IC50 values ranging from 12.35 to 24.21?g/ml. While the essential oils of A. cherimola showed IC50 values ranging from 7.67 to 9.22?g/ml. Leaf oils and ethanol extractives showed appreciable antibmicobial activity with variable MIC ranging from 30 to 315µg/ml. These findings suggest that A. cherimola essential oil and ethanol extract have great potential as a natural medicine for cancers and microbial infections. Keywords:Annona cherimola; A. squamosa;, A. muricata ; A. glabra;   Annonaceae;   DNA, essential   oil   composition; cytotoxicity; antimicobia

DNA Fingerprinting, Chemical Composition, Antitumor and Antimicrobial Activities of the Essential Oils and Extractives of four Annona Species from Egypt

The leaf essential oils of four members of the Annonaceae grown in Egypt (namely; Annona cherimola, A. squamosa, A. muricata and A. glabra) have been obtained by hydrodistillation and analyzed by GC-MS in order to compare and contrast the volatile chemical compositions of these species.  The essential oils were screened for in-vitro cytotoxic activity against breast cancer (MCF-7), colon cancer (CACO) and liver cancer (HEPG2) cell lines and antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeuroginosa, Aspergillus flavus and Candida albicans. beta-elemene (25.02%), beta-caryophyllene (37.11%), bicycloelemene (23.58%) and and beta-gurjunene (42.49%), were the major constituents of Annona cherimola, A. squamosa, A. muricata and A. glabra, respectively. Ethanol extracts showed highly significant cytotoxic activities (low IC50) much more than results displayed by essential oils on breast (MCF-7), colon (CACO) and liver (HEPG2) carcinoma cell lines. Relative to breast carcinoma cell line (MCF-7), The IC50 values of ethanol extracts were 3.43, 3.89 and 4.34 ?g/ml for A. cherimola, A. squamosa and A. muricata ethanol extractives respectively. While colon carcinoma cell line (CACO) displayed IC50 values 2.82, 2.97, 3.58 and 3.89 ?g/ml for A. muricata, A. cherimola, A. glabra and A. squamosa, respectively. Liver carcinoma cell line (HEPG2) exhibited IC50 valus of 3.12, 3.43 and 3.73 for A. squamosa, A. muricata and A. cherimola, respectively. Three of the four leaf essential oils showed notable invitro cytotoxic activity. Essential oils of A. glabra, A. muricata and A. squamosa showed moderate cytotoxic activities with IC50 values ranging from 12.35 to 24.21?g/ml. While the essential oils of A. cherimola showed IC50 values ranging from 7.67 to 9.22?g/ml. Leaf oils and ethanol extractives showed appreciable antibmicobial activity with variable MIC ranging from 30 to 315µg/ml. These findings suggest that A. cherimola essential oil and ethanol extract have great potential as a natural medicine for cancers and microbial infections. Keywords:Annona cherimola; A. squamosa;, A. muricata ; A. glabra;   Annonaceae;   DNA, essential   oil   composition; cytotoxicity; antimicobia

5-azacytidine promotes microspore embryogenesis initiation by decreasing global DNA methylation,but prevents subsequent embryo development in rapeseed and barley

17 p.-10 fig.Microspores are reprogrammed by stress in vitro toward embryogenesis. This process is an important tool in breeding to obtain double-haploid plants. DNA methylation is a major epigenetic modification that changes in differentiation and proliferation. We have shown changes in global DNA methylation during microspore reprogramming. 5-Azacytidine (AzaC) cannot be methylated and leads to DNA hypomethylation. AzaC is a useful demethylating agent to study DNA dynamics, with a potential application in microspore embryogenesis. This work analyzes the effects of short and long AzaC treatments on microspore embryogenesis initiation and progression in two species, the dicot Brassica napus and the monocot Hordeum vulgare. This involved the quantitative analyses of proembryo and embryo production, the quantification of DNA methylation, 5-methyl-deoxy-cytidine (5mdC) immunofluorescence and confocal microscopy, and the analysis of chromatin organization (condensation/decondensation) by light and electron microscopy. Four days of AzaC treatments (2.5 μM) increased embryo induction, response associated with a decrease of DNA methylation, modified 5mdC, and heterochromatin patterns compared to untreated embryos. By contrast, longer AzaC treatments diminished embryo production. Similar effects were found in both species, indicating that DNA demethylation promotes microspore reprogramming, totipotency acquisition, and embryogenesis initiation, while embryo differentiation requires de novo DNA methylation and is prevented by AzaC. This suggests a role for DNA methylation in the repression of microspore reprogramming and possibly totipotency acquisition. Results provide new insights into the role of epigenetic modifications in microspore embryogenesis and suggest a potential benefit of inhibitors, such as AzaC, to improve the process efficiency in biotechnology and breeding programs.Work supported by projects (references BFU2008-00203, BFU2011-23752, AGL2014-52028-R) funded by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (ERDF/FEDER). AAET is recipient of a predoctoral fellowship of the JAE-Pre Program of the Spanish National Research Council, CSIC (JAEPre2010-052), cofunded by ERDF/FEDER.Peer reviewe

On the approximations to fractional nonlinear damped Burger’s-type equations that arise in fluids and plasmas using Aboodh residual power series and Aboodh transform iteration methods

Damped Burger’s equation describes the characteristics of one-dimensional nonlinear shock waves in the presence of damping effects and is significant in fluid dynamics, plasma physics, and other fields. Due to the potential applications of this equation, thus the objective of this investigation is to solve and analyze the time fractional form of this equation using methods with precise efficiency, high accuracy, ease of application and calculation, and flexibility in dealing with more complicated equations, which are called the Aboodh residual power series method and the Aboodh transform iteration method (ATIM) within the Caputo operator framework. Also, this study intends to further our understanding of the dynamic characteristics of solutions to the Damped Burger’s equation and to assess the effectiveness of the proposed methods in addressing nonlinear fractional partial differential equations. The two proposed methods are highly effective mathematical techniques for studying more complicated nonlinear differential equations. They can produce precise approximate solutions for intricate evolution equations beyond the specific examined equation. In addition to the proposed methods, the fractional derivatives are processed using the Caputo operator. The Caputo operator enhances the representation of fractional derivatives by providing a more accurate portrayal of the underlying physical processes. Based on the proposed two approaches, a set of approximations to damped Burger’s equation are derived. These approximations are discussed graphically and numerically by presenting a set of two- and three-dimensional graphs. In addition, these approximations are analyzed numerically in several tables, including the absolute error for each approximate solution compared to the exact solution for the integer case. Furthermore, the effect of the fractional parameter on the behavior of the derived approximations is examined and discussed

On Pairwise λ-Open Soft Sets and Pairwise Locally Closed Soft Sets

Kandil and his colleagues [10], introduced the notion of -closed soft set by involving -soft set and -closed soft set. In this paper, we give some additional properties of -closed soft sets. We also introduce and study a related new class of -spaces which lies between  and  . Moreover, we show that there exists a very important relation between the notion of -closed soft sets and the  property, ,  , . In addition, we offer the notion of -locally closed soft sets and we investigate a related new pairwise soft separation axiom  which is independent from . The relationships between the -closed soft sets and the -locally closed soft sets are obtained. Furthermore, we introduce the notion of -open soft sets and we construct supra soft topology associated with the class of -open soft sets and we present pairwise soft separation axioms related to such soft sets, namely . We provide some illustrative examples to support the results

Breather patterns and other soliton dynamics in (2+1)-dimensional conformable Broer-Kaup-Kupershmit system

In this work, the Extended Direct Algebraic Method (EDAM) is utilized to analyze and solve the fractional (2+1)-dimensional Conformable Broer-Kaup-Kupershmit System (CBKKS) and investigate different types of traveling wave solutions and study the soliton like-solutions. Using the suggested method, the fractional nonlinear partial differential equation (FNPDE) is primarily reduced to an integer-order nonlinear ordinary differential equation (NODE) under the traveling wave transformation, yielding an algebraic system of nonlinear equations. The ensuing algebraic systems are then solved to construct some families of soliton-like solutions and many other physical solutions. Some derived solutions are numerically analyzed using suitable values for the related parameters. The discovered soliton solutions grasp vital importance in fluid mechanics as they offer significant insight into the nonlinear behavior of the targeted model, opening the way for a deeper comprehension of complex physical phenomena and offering valuable applications in the associated areas