D6.1 FERTIMANURE Plan for Exploitation and Dissemination of Results – 1st version

The Preliminary Plan for the Exploitation and Dissemination of Results (PEDR) is a strategic document for setting the impact pathway of FERTIMANURE since the project beginning. The main objective of FERTIMANURE project is to develop, integrate, test and validate innovative Nutrient Management Strategies to efficiently recover mineral nutrients and other relevant products with agronomic value (organic amendments and biostimulants) from animal manure, to finally obtain reliable and safe fertilisers that can compete in the European fertilizers market. FERTIMANURE project will demonstrate the efficiency and effectiveness of the obtained BBFs and TMFs and their ability to replace current mineral fertilisers, including: (i) Tests under controlled conditions (incubation and pot-tests): Assessment of: (1) N release patterns; (2) P plant availability; (3) Biological activated organic amendments as plant growth promoters; (4) Biostimulants assessment as plant growth promoters for the nutrient uptake and tolerance against hydric & saline stress. Crops under assessment: Wheat, barley, maize, fodder maize, grass, ray grass. (ii) Field validation in real environment: Agronomic performance in quadruplicate-randomized block design; Environmental performance, including nutrient efficiency vs losses. Crops under assessment: Wheat, barley, maize, grass, corn, spinach, potatoes, tomatoes, vines. FERTIMANURE project specifically provides 3 innovative nutrient management strategies: 1) Direct use of the BBFs obtained by treating manure with innovative technologies; 2) Centralised TMFs production and 3) On-farm TMFs production. This document is the first version (M6) of the Plan for Exploitation and Dissemination of Results (PEDR) of FERRTIMANURE project. The contents of the first release include an overview of the concepts of Communication, Dissemination and Exploitation relative strategies and action plans that the consortium will follow to promote the project, to foster the knowledge of its results and to ensure their uptake for future business opportunities. All these activities aim to help maximize the impact of results. The PEDR will be updated during the project in the following months: M26, M36 and M48.H2020 (Grant no. 862849

D8.2 Data Management Plan – 1st version

The Data Management Plan (DMP) sets out the procedures and plans for handling data generated through the FERTIMANURE project, and is intended to be a manual for guiding and facilitating general data management and data storage needs for FERTIMANURE consortium members. This document also aims to clarify the obligations that beneficiaries have in terms of data standards, storage and protection, during the project and beyond with the objective of facilitating the exploitation of the results. It is intended to be a continuously evolving document, and hence can be adapted as the project progresses. This adaption will allow for any developments that have not been foreseen at the project start, and will be facilitated through review by the consortium members via the consortium meetings (Project Management Board). The expectation is that the project will produce 5 types of data, which include: 1) Documents generated for project analyses or deliverables 2) Schematics and P&IDs of the different FERTIMANURE on-farm pilots 3) Experimental, including: a. Data collected on pilot operation and processes b. Data on the properties and quality of products obtained from the pilot plants c. Experimental data collected in the agronomic trials 4) Data collected for undertaking the LCA and sustainability analyses 5) Software tools Due to the particular nature of these data types, the way each are generated, and the limitations for sharing and publication of each, the following sections outline the ways these data will be gathered, maintained, shared, and published.H2020 (Grant no. 862849

D6.3 Inventory of stakeholder groups relevant for BBFs and market uptake – 1st version

The FERTIMANURE project includes 20 partners from 7 EU countries, Argentina and Chile. EU countries participating in the project include France, Germany, Spain, Italy, Belgium, The Netherlands, and Croatia (Figure 1). CELAC region is represented by Argentina. The project consortium is geographically well distributed across the Member States of the EU-27 with an intention to cover diverse range of agricultural and nutrient management practices and includes stakeholders with different knowledge background and fertiliser needs. CELAC region is represented by the largest CELAC member state – Argentina. The main objective of FERTIMANURE is to develop, integrate, test, and validate innovative nutrient management strategies to efficiently recover mineral nutrients and other relevant products with agronomic value (organic amendments and biostimulants) from animal manure, to finally obtain reliable and safe fertilisers that can compete in the European fertilisers market.H2020 (Grant no. 862849

D1.2 Report on the market landscape analysis and end-user preferences in the project participating EU states

This report is part of the Horizon 2020 project Fertimanure – “From Farm to Market: Upcycling manure to improved fertilising products”. The project is coordinated by BETA Technological Centre at the University of Vic in Catalonia, Spain, and includes together 20 partners from 7 EU countries, Argentina and Chile. It includes universities, research centres, cluster organisations, public bodies, SMEs and NGOs. This report is part of Work Package 1: FERTIMANURE framework and as such constitutes the required deliverable D1.2 Report on the market landscape analysis and end-user preferences in the project participating EU states, Task 1.2. Market landscape analysis and end-users preferences.H2020 (Grant No. 862849

Radiosynthesis and biological investigation of a novel fluorine-18 labeled benzoimidazotriazine- based radioligand for the imaging of phosphodiesterase 2A with positron emission tomography

A specific radioligand for the imaging of cyclic nucleotide phosphodiesterase 2A (PDE2A) via positron emission tomography (PET) would be helpful for research on the physiology and disease-related changes in the expression of this enzyme in the brain. In this report, the radiosynthesis of a novel PDE2A radioligand and the subsequent biological evaluation were described. Our prospective compound 1-(2-chloro-5-methoxy phenyl)-8-(2-fluoropyridin-4-yl)-3- methylbenzo[e]imidazo[5,1-c][1,2,4]triazine, benzoimidazotriazine (BIT1) (IC50 PDE2A = 3.33 nM; 16-fold selectivity over PDE10A) was fluorine-18 labeled via aromatic nucleophilic substitution of the corresponding nitro precursor using the K[18F]F-K2.2.2-carbonate complex system. The new radioligand [18F]BIT1 was obtained with a high radiochemical yield (54 ± 2%, n = 3), a high radiochemical purity (≥99%), and high molar activities (155–175 GBq/μmol, n = 3). In vitro autoradiography on pig brain cryosections exhibited a heterogeneous spatial distribution of [18F]BIT1 corresponding to the known pattern of expression of PDE2A. The investigation of in vivo metabolism of [18F]BIT1 in a mouse revealed sufficient metabolic stability. PET studies in mouse exhibited a moderate brain uptake of [18F]BIT1 with a maximum standardized uptake value of ~0.7 at 5 min p.i. However, in vivo blocking studies revealed a non-target specific binding of [18F]BIT1. Therefore, further structural modifications are needed to improve target selectivity