Brewery spent yeast insoluble residue as microcarriers for bioactive compound

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Cultivation of candida sp. LEB-M3 in glycerol: lipid accumulation and prediction of biodiesel quality parameters

The quality of biodiesel from lipids produced by the yeast Candida sp. LEB-M3 was predicted, by the use of mathematical models for parameters that specify quality as a function of the fatty acid profile. The lipid production was studied according to the experimental design methodology, for different cultivation conditions for agitation and aeration. Lipid compositions were affected by the cultivation conditions, and the agitation presented a positive effect for the formation of monounsaturated fatty acids and negative effect for saturated fatty acids. Aeration had a positive effect on the formation of polyunsaturated fatty acids. According to the predictions by the mathematical models, the cetane number varied from 61 to 67, the oxidative stability from 11 to 17 h, the iodine index from 55 to 75 gI2/100 g, density from 852 to 868 kg/m3. All cultivation conditions led to lipid compositions, whose predicted bioparameter values indicate that biodiesel from this lipid source should present current standard quality161416422CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQSem informaçã

Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant rhizopus oryzae lipase

The recombinant Rhizopus oryzae lipase (1–3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved183175180CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESSem informação12891-13-

Caracterização cinética e termodinâmica de β-galactosidase de Kluyveromyces marxianus CCT 7082 fracionada com sulfato de amônio

A β-galactosidase é uma enzima importante que atua na hidrólise da lactose, podendo ser empregada na obtenção de alimentos destinados a consumidores intolerantes a esse dissacarídeo. A levedura Kluyveromyces marxianus apresenta bom rendimento de crescimento, além de ser um micro-organismo seguro em aplicações industriais, e tem sido utilizada para produção da enzima por cultivo submerso. A β-galactosidase obtida foi fracionada com sulfato de amônio e caracterizada quanto a temperatura e pH ótimos, estabilidade térmica, valores D e z, e parâmetros cinéticos e termodinâmicos. A temperatura e pH ótimos foram de 45-50 °C e 7,0, respectivamente. A energia de ativação da reação enzimática foi de 9,8 kcal.mol–1 e da reação de desativação, 64,2 kcal.mol–1. Os valores de Km e Vmax obtidos foram de 3,7 mM e 99,0 U.mL–1, respectivamente. A energia livre de Gibbs reduziu com o aumento de temperatura, sendo a enzima mais estável a 30 °C (ΔG* = 106,8 kJ.mol–1). A entalpia foi de 313,04 kJ.mol–1 e a entropia 0,68 kJ.mol–1.k–1. O valor D confirmou que a enzima foi mais estável em temperaturas próximas de 30 °C (D = 11.513,0 min) e o valor z foi de 5,8 °C.β-galactosidase is an important enzyme that acts on lactose hydrolysis and can be used to obtain food for consumers intolerant to this disaccharide. The yeast Kluyveromyces marxianus presents a good growth yield, is a safe microorganism for industrial applications and has been used for enzyme production using the submerged process. The β-galactosidase obtained was fractionated with ammonium sulphate and characterized with respect to its optimum temperature and pH, thermal stability and its D and z values, as well as its kinetic and thermodynamic parameters. The optimum temperature and pH were 45-50 °C and 7.0, respectively. The activation energy and the deactivation reaction of the enzymatic reaction were, respectively, 9.8 kcal.mol–1 and 64.2 kcal.mol–1. The Km and Vmax values obtained were 3.7 mM and 99.0 U.mL–1, respectively. The Gibbs free energy decreased with increasing temperature and the enzyme was more stable at 30 °C (ΔG* = 106.8 kJ.mol–1). The enthalpy was 313.04 kJ.mol–1 and entropy 0.68 kJ.mol.k–1. The D value confirmed that the enzyme was more stable at temperatures around 30 °C (D = 11,513.0 min) and the z value was 5.8 °C

Enzymatic Biodiesel Synthesis From Yeast Oil Using Immobilized Recombinant Rhizopus Oryzae Lipase.

The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.183175-8