Oxidative stress response of black tiger shrimp (<i>Penaeus monodon</i>) to enrofloxacin and to culture system

In Vietnam, enrofloxacin is one of the most commonly used antibiotics in shrimp farms. Although the European Union set a maximum residue limit (MRL) of 100 µg/kg for enrofloxacin and its metabolite ciprofloxacin in edible tissues of aquatic animals for human consumption, only few data are available on its potential deleterious effects on shrimp. This study aimed to investigate the impacts of enrofloxacin on oxidative stress in hepatopancreas and gills of black tiger shrimp (Penaeus monodon). Experiments were conducted under laboratory and field conditions, for which two different culture systems were distinguished (intensive and improved extensive culture systems). Shrimp (average weight of 10 g) were fed with medicated-feed containing 4 g enrofloxacin/kg for 7 days. We showed that enrofloxacin medication caused very small changes in oxidative stress status of the shrimp. However, the culture system has a significant impact. The basal level of hepatopancreas lipid peroxidation (LPO) was higher in intensive than in improved extensive culture system, while glutathione S-transferase (GST) activity was lower in the former system. In addition, an elevated gill catalase (CAT) activity was observed in the shrimp sampled from intensive culture. In conclusion, LPO, CAT and GST can be proposed as biomarkers to point out the general stress status of the shrimp and to discriminate between shrimp cultivated in intensive and improved extensive culture systems

Consumption and nutritional quality of grilled pork purchased from open road-side restaurants of Benin

This study focused on the determination of consumption habits and nutritional composition of ready-to-eat grilled pork from Benin. A face-to-face survey of 372 grilled pork consumers using a semi-structured questionnaire was carried out in the same municipalities where 24 samples of grilled pork were collected for physico-chemical characterisation. Three hundred out of 372 (80.6 %) consumers eating grilled pork at least once per month were selected to estimate the daily intake of protein, lipid and fatty acids. Grilled pork consumption ranged between 0.7 g/day and 342.9 g/day with a median of 28.6 g/day. Protein and lipid contents ranged between 19.5 % and 69.5 % of dry weight and between 27.9 % and 82.1 % of dry weight, respectively. Fatty acid profile showed that linoleic acid was the most abundant omega 6-PUFA (Polyunsaturated fatty acid). The consumption of high quantity of grilled pork (342.9 g/day) enables to cover 25 % (for women) and 31 % (for men) of adult requirement of PUFA (11−22 g/day for women and 14−28 g/day for men). The omega 6 daily intake from high grilled pork consumption contributed to satisfy about 15 % of adult requirement of omega 6 fatty acids for both genders while the omega 3 daily intake from high grilled pork consumption was over 80 % for both genders

Validation of the Analytical Procedure for the Determination of Malondialdehyde and Three Other Aldehydes in Vegetable Oil Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS) and Application to Linseed Oil

Secondary oxidation products of fatty acids, mainly aldehydes, are susceptible to cause significant deterioration in chemical, sensory and nutritional food properties, as well as adverse health effects. An analytical method involving separation by liquid chromatography coupled to the detection by tandem mass spectrometry (LC-MS/MS) has been developed to evaluate the concentration of four aldehydes in oil samples: malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE) and 2,4-decadienal (2,4-DECA). The optimisation of the extraction, derivation, detection and quantification has been finalised for coconut oil, used as a model of vegetable oils. The method has been validated according to the criteria and procedure described in international standards. The evaluated parameters include specificity/selectivity, recovery, precision, accuracy, uncertainty, limits of detection and quantification, using the concept of accuracy profiles. These parameters have been evaluated during experiments planned on different non-consecutive days with coconut oil spiked at different levels of concentration. The validation of the developed analytical method showed that it is possible to analyse MDA, 4-HHE, 4-HNE and 2,4-decadienal in oil samples, in the same run, with a very good accuracy for MDA, and a defined accuracy at specified concentrations for the three other aldehydes. The accuracy profile of MDA showed a recovery rate of 100 % (±1) and a maximum coefficient of variation for the intermediate precision of 14 % at 0.15 mg kg−1. For the three other aldehydes, recovery rates ranged between 79 and 101 % and coefficient of variation for the intermediate precision between 13 and 23 %. In first pressure linseed oil, stored for several days at 60 °C according to the Schaal oven test, it was shown that 4-HHE was the most produced aldehyde, reaching levels of 85 and 382 μmol kg−1 after 12 and 24 days, respectively, versus levels of 18 and 28 μmol MDA kg−1 of oil, respectively, and 17 and 51 μmol 4-HNE kg−1 of oil. © 2014, Springer Science+Business Media New York

Monitoring Antibiotic Use and Residue in Freshwater Aquaculture for Domestic Use in Vietnam.

Contains fulltext : 152490.pdf (Publisher’s version ) (Open Access

Development of an HPTLC method for determination of hypoglycin A in aqueous extracts of seedlings and samaras of Acer species

International audienceHypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree ( Acer pseudoplatanus ). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo, is claimed to be present where AM cases were reported in the US. For unknown reasons, occurrence of this disease has increased. It is important to define environmental key factors that may influence toxicity of samaras from Acer species. In addition, the content of HGA in seedlings needs to be determined since AM outbreaks, during autumn period when the seeds fall but also during spring when seeds are germinating. The present study aims to validate a reliable method using high performance thin layer chromatography for determination and comparison of HGA in samaras and seedlings. The working range of the method was between 20 μg HGA to 408 μg HGA per ml water, corresponding to 12 - 244 mg/kg fresh weight or 40 - 816 mg/kg dry weight, taking into account of an arbitrary average dry matter content of 30%. Instrumental limit of detection and limit of quantification were of 10 μg HGA/ml and 20 μg HGA/ml water, respectively. Instrumental precision was 4% (RSD on 20 repeated measurements) while instrumental accuracy ranged between 86% and 121% of expected value. The HGA recovery of the analytical method estimated from spiked samaras and seedlings samples ranged between 63 and 103%. The method was applied to 9 samples of samaras from Acer pseudoplatanus, A. platanoides and A. campestre and 5 seedlings samples from A. pseudoplatanus. The results confirm detection of HGA in samaras from A. pseudoplatanus and the absence of detection in samaras of other tested species. They also suggest that detected levels of HGA are highly variable. This confirmed the suitability of the method for HGA detection in samaras or seedling

Effects of aflatoxins and fumonisins, alone or in combination, on performance, health, and safety of food products of broiler chickens, and mitigation efficacy of bentonite and fumonisin esterase

The current study evaluated the effects of feeding diets contaminated with aflatoxin B1 (AFB1), fumonisins (FBs), or both on the performance and health of broiler chickens and the safety of their food products as well as the efficacy of bentonite and fumonisin esterase to mitigate the effects of these mycotoxins under conditions representative for sub-Saharan Africa (SSA). Four hundred one-day-old Cobb 500 broiler chickens were randomly assigned to 20 treatments with either a control diet, a diet with moderate AFB1 (60 μg/kg feed) or high AFB1 (220 μg/kg feed), or FBs (17,430 μg FB1+FB2/kg feed), alone or in combination, a diet containing AFB1 (either 60 or 220 μg/kg) and/or FBs (17,430 μg FB1+FB2/kg) and bentonite or fumonisin esterase or both, or a diet with bentonite or fumonisin esterase only. The experimental diets were given to the birds from day 1 to day 35 of age, and the effects of the different treatments on production performance were assessed by feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR). Possible health effects were evaluated through blood biochemistry, organ weights, mortality, liver gross pathological changes, and vaccine response. Residues of aflatoxins (AFB1, B2, G1, G2, M1 and M2) were determined in plasma, muscle, and liver tissues using validated UHPLC-MS/MS methods. The results obtained indicated that broiler chickens fed high AFB1 alone had poor FCR when compared to a diet with both high AFB1 and FBs (p = 0.0063). Serum total protein and albumin from birds fed FBs only or in combination with moderate or high AFB1 or detoxifiers increased when compared to the control (p < 0.05). Liver gross pathological changes were more pronounced in birds fed contaminated diets when compared to birds fed the control or diets supplemented with mycotoxin detoxifiers. The relative weight of the heart was significantly higher in birds fed high AFB1 and FBs when compared to the control or high AFB1 only diets (p < 0.05), indicating interactions between the mycotoxins. Inclusion of bentonite in AFB1-contaminated diets offered a protective effect on the change in weights of the liver, heart and spleen (p < 0.05). Residues of AFB1 were detected above the limit of quantification (max: 0.12 ± 0.03 μg/kg) in liver samples only, from birds fed a diet with high AFB1 only or with FBs or the detoxifiers. Supplementing bentonite into these AFB1-contaminated diets reduced the levels of the liver AFB1 residues by up to 50%. Bentonite or fumonisin esterase, alone, did not affect the performance and health of broiler chickens. Thus, at the doses tested, both detoxifiers were safe and efficient for use as valid means of counteracting the negative effects of AFB1 and FBs as well as transfer of AFB1 to food products (liver) of broiler chickens