DNA and bone structure preservation in medieval human skeletons

Morphological and ultrastructural data from archaeological human bones are scarce, particularly data that have been correlated with information on the preservation of molecules such as DNA. Here we examine the bone structure of macroscopically well-preserved medieval human skeletons by transmission electron microscopy and immunohistochemistry, and the quantity and quality of DNA extracted from these skeletons. DNA technology has been increasingly used for analyzing physical evidence in archaeological forensics; however, the isolation of ancient DNA is difficult since it is highly degraded, extraction yields are low and the co-extraction of PCR inhibitors is a problem. We adapted and optimized a method that is frequently used for isolating DNA from modern samples, Chelex® 100 (Bio-Rad) extraction, for isolating DNA from archaeological human bones and teeth. The isolated DNA was analysed by real-time PCR using primers targeting the sex determining region on the Y chromosome (SRY) and STR typing using the AmpFlSTR® Identifiler PCR Amplification kit. Our results clearly show the preservation of bone matrix in medieval bones and the presence of intact osteocytes with well preserved encapsulated nuclei. In addition, we show how effective Chelex® 100 is for isolating ancient DNA from archaeological bones and teeth. This optimized method is suitable for STR typing using kits aimed specifically at degraded and difficult DNA templates since amplicons of up to 250 bp were successfully amplified

DANISH NATIONAL MUSEUM: INTERACTIVE MARKETING FOR A - YOUNGER GENERATION

The Danish National Museum in Copenhagen sponsored a project for the creation of a new electronic marketing device aimed to support museum visitors in the age cohort 14 to 35. Internet technology was the most applicable method for creating an interactive advertising tool according to both the online survey and literature review. The project team used Flash 8 to construct a website that allows for users to create personal Virtual Exhibits with museum photographs that can be printed and shared

2007-08 WPI SAE Baja Vehicle

The objective for this project was to improve upon the 2006-2007 WPI Baja Vehicle for participation in SAE's 2008 Baja Series competitions. Several major vehicle systems were redesigned using both mathematical and computer-aided modeling and simulation. These included the drivetrain, front and rear suspensions, and rear chassis. Improvements in these areas resulted in a lighter and more powerful vehicle that remained as safe and as durable as the previous design. The new parts were fabricated by the team using WPI facilities and with the help of local businesses. The finished vehicle will compete in Illinois (May 08) and Montreal (June 08)