マウス原腸胚形成におけるCables2の役割

筑波大学 (University of Tsukuba)201

STUDY OF EFFECT OF FRICTION STIR WELDING PARAMETERS ON IMPACT ENERGY OF AA7075-T6

The influences of the tool rotation speed (denoted w) and the welding speed (denoted v) on the impact energy at the representative zones in the friction stir welding (FSW) of AA7075-T6 were investigated. Here, the standard V–Notched specimens were applied in which the notches were addressed at the stirred zone (SZ), the heat affected zones (HAZ) in both the advancing side and the retreating side and the mixed zone (MZ). The experimental results showed that, in all cases, the lowest impact energy is located at the stirred zone and that energy seems to be increased from the SZ to the HAZ across the welding. Furthermore, it is also found that the impact energy is decreased when the ratio of rotation speed to welding speed (w/v) is increased. The microstructure, the temperature distribution, and the hardness in and around the welded zone were considered and discussed

Endoparasites in pigs raised in smallholder farms in Hung Yen province of Vietnam

As being part of an ongoing project, assessing animal health and food safety risks in smallholder pig value chains in Hung Yen province of Vietnam, the presence of endoparasites was investigated in a repeated study. Three communes, with a total of 545 smallhoder pig farms, from three districts were selected. In each commune, ten farms were randomly chosen. On farm data including farm management and biosecurity measures were recorded by using a checklist and observations. From each farm, one pooled fecal sample was collected from a randomly chosen barn monthly and followed from June to December 2014 for seven months. Fecal samples were analyzed for intestinal parasites by floatation and sedimentation methods. The intensity of most commonly seen parasites was determined by the McMaster counting technique. In 198 collected fecal samples, six types of parasites were detected, namely Eimeria 89 (44.95%), Ascaris suum 43 (21.72%), Strongyloides sp. 41 (20.71%), Trichocephalus suis 19 (9.60%), Fasciolopsis buski 10 (5.05%), and Fasciola 2 (1.01%). Highest intensities were observed for Eimeria (maximum 80900 oocyst/g), followed by Ascaris suum (2400 egg/g), Strongyloides sp. (1900 egg/g), and Trichocephalus suis (1300 egg/g); the others were reported at much lower intensities. Infection levels and intensities of the parasites tended to be higher during the hot months, June to September. No significant difference between communes was found. At least one type of parasites was present in 137 samples (69.19%). Most of farms (96.67%) were positive for at least one type of parasites in at least one sample. There was a significant difference in infection level of endoparasites between biogas and non-biogas farms. The general high parasite burden reported in this study will be addressed in future interventions which will include parasite control apart from other measures

EFL STUDENT’S PROBLEMS WITH PARAGRAPH WRITING AT TAY DO UNIVERSITY, VIETNAM

This paper studied problems in EFL students’ paragraph writing. The research participants were first-year students of English in Tay Do University (TDU). To achieve the desired aims of the current study, the researcher combined both qualitative and quantitative methods, using questionnaires, interviews, and observations as the main instruments. The researcher used the package of social sciences (SPSS) for analysis. Research results showed that the freshmen at TDU often encountered problems in writing paragraphs. These accounted for grammatical structures, vocabulary, and coherence. Basing on the research results, students could recognize their difficulties when writing and find some new ways to overcome them. It is suggested that teachers should find and update some new teaching methods so as to develop students’ paragraph writing skills. Article visualizations

Reproductive biology of the mudskipper Boleophthalmus boddarti in Soc Trang

The mudskipper Boleophthalmus boddarti is an amphibious fish widely distributed in the mudflat regions in Asia, but its reproductive biology was poorly stydied. This study was conducted in the Tran De, Soc Trang, Vietnam, from March 2013 to February 2014, to investigate reproductive parameters of B. boddarti. Data analysis based on a total of 360 collected fish (188 females and 172 males) suggest that the sex ratio of this mudskipper was not significantly different between dry and wet seasons. This fish was a multiple reproducer and spawned in the wet season with a peak spawning in three main months of this season (August to October) as difference stages of oocytes were observed in the ovaries. The male B. boddarti released sperms during the reproductive season since different type of spermatogonia were found in stage V testis sections. This fish reached 11.52 cm total length at first sex maturation and was high fecundity (9,800-33,800 eggs), indicating a possible adaptation of this species to the monsoonal climate. The understanding of the reproductive biology of B. boddarti provides useful knowledge of its reproductive adaptation to the mudflat toward sustainable management in the study region

Enzimatska obrada iskorištenih listića zelenog čaja i njihova primjena u proizvodnji čajnog peciva s velikim udjelom prehrambenih vlakana

Research background. By-products of food industry have been studied as sources of high fibre and antioxidant ingredients for healthy food products, because of their economic and environmental benefits. However, the soluble dietary fibre content of these materials is usually lower than the recommended value that is claimed to bring positive health effects. Enzymatic treatment could be an efficient method for modifying insoluble and soluble dietary fibre contents of these materials. The purpose of this study is to investigate the effects of enzymatic treatment conditions on soluble, insoluble and total dietary fibre mass fractions in spent green tea leaves, and evaluate the quality of dough and cookies when different mass fractions of untreated and treated leaves were added to the recipe. Experimental approach. The mass fractions of soluble, insoluble and total dietary fibre in spent tea leaf powder was evaluated after the leaves were treated with cellulase amount of 0−25 U/g for 0 to 2 h. Wheat flour was replaced by untreated and treated spent tea leaf powder at 0, 10, 20, 30 and 40% in cookie formulation. Textural properties of dough, proximate composition, physical properties and overall acceptability of cookies were analysed. Results and conclusions. The appropriate conditions for enzymatic treatment were enzyme loading of 20 U/g and biocatalytic time of 1.5 h, under which the mass fraction of soluble dietary fibre in spent tea leaves increased by 144.5% compared to that of the control sample. The addition of spent tea leaves led to the increase in dough hardness. Increase in the spent tea leaf amount also enhanced fibre mass fraction, antioxidant activity and hardness of cookies but reduced their overall acceptability. Moreover, the enzymatic treatment of spent tea leaves improved the soluble to total dietary fibre ratio of the cookies, which influenced their textural properties and health benefits. The cookies with added 20% untreated or treated spent tea leaves were overall accepted by the panel. Novelty and scientific contribution. For the first time, spent tea leaves have been treated with enzymes to improve their soluble to total dietary fibre ratio. The treated spent tea leaves are a new promising high-fibre antioxidant ingredient for cookie preparation.Pozadina istraživanja. Nusproizvodi prehrambene industrije ispituju se iz ekonomskih i ekoloških razloga kao izvori sastojaka s velikim udjelom prehrambenih vlakana i antioksidacijskim učinkom koji se mogu upotrijebiti u proizvodnji zdrave hrane. Međutim, udjel topljivih prehrambenih vlakana u nusproizvodima je obično manji od preporučenih vrijednosti koje mogu imati pozitivan učinak na zdravlje. Enzimatska obrada bi mogla biti učinkovita metoda modificiranja netopljivih i topljivih prehrambenih vlakana iz otpada prehrambene industrije. Svrha je ovog rada bila ispitati utjecaj različitih uvjeta enzimatske obrade na masene udjele topljivih, netopljivih i ukupnih prehrambenih vlakana u iskorištenim listićima zelenog čaja, te procijeniti kakvoću tijesta i čajnog peciva s dodatkom različitih masenih udjela obrađenih i neobrađenih listića. Eksperimentalni pristup. Maseni udjeli topljivih, netopljivih i ukupnih prehrambenih vlakana u prahu od iskorištenih listića zelenog čaja mjereni su nakon njihove obrade s 0–25 U/g celulaze tijekom 0 do 2 h. U smjesi za čajno pecivo je pšenično brašno zamijenjeno s 0, 10, 20, 30 i 40 % obrađenog ili neobrađenog praha iskorištenih listića zelenog čaja. Ispitani su tekstura tijesta te kemijski sastav, fizikalna svojstva i ukupna prihvatljivost dobivenog čajnog peciva. Rezultati i zaključci. Pri povoljnim uvjetima enzimatske obrade, a to su 20 U/g enzima i vrijeme trajanja biokatalitičkog procesa od 1,5 sata, maseni udjel topljivih prehrambenih vlakana u iskorištenim listićima zelenog čaja porastao je za 144,5 % u usporedbi s kontrolnim uzorkom. Dodatkom iskorištenih listića zelenog čaja povećala se tvrdoća tijesta. Povećanjem količine listića čaja povećali su se i maseni udjel vlakana, antioksidacijska svojstva i tvrdoća čajnog peciva, ali se smanjila njihova prihvatljivost. Osim toga, enzimatskom se obradom iskorištenih listića zelenog čaja poboljšao omjer topljivih i ukupnih vlakana u čajnom pecivu, što je utjecalo na njegovu teksturu i pozitivan učinak na zdravlje. Panel ocjenjivača je okarakterizirao čajno pecivo s dodatkom 20 % obrađenih ili neobrađenih iskorištenih listića čaja kao prihvatljive. Novina i znanstveni doprinos. Prvi put je enzimatskom obradom iskorištenih listića zelenog čaja poboljšan njihov omjer topljivih i ukupnih prehrambenih vlakana. Obrađeni iskorišteni listići zelenog čaja novi su obećavajući sastojak s antioksidacijskim svojstvima koji se može upotrijebiti u pripremi čajnog peciva

HMU fluorinze mouthwash enhances enamel remineralization: An in vitro study

BACKGROUND: Fluoride therapy has long been used extensively to prevent dental caries. Fluoride appears in variety of dental care products such as mouthrinse, dentifrice, gel, etc. HMU fluorinze is the first mouthwash containing fluoride in Vietnam. AIM: This research was conducted to evaluate the efficacy of HMU Fluorinze mouthwash on remineralizing enamel in laboratory conditions. METHODS: 20 third molars teeth were cleaned and covered with nail polish , except for a 3x3 mm square on their buccal surfaces. These teeth went through two steps: demineralization using Coke and remineralization for 20 days: 1) using standard calcifying solution (control group) and 2) using standard calcifying solution + HMU Fluorinze mouthwash 2 times/day (experimental group). The mineralization index of enamel structure after demineralization and remineralization was assessed by DIAGNOdent pen 2190. RESULTS: The mineralization indexes of the control group and experimental group at baseline were 3.65 ± 0.76 and 3.35 ± 0.64, after demineralization were in turn of 21.78 ± 4.48 and 20.25 ± 2.26; and after remineralization were 6.30 ± 1.03 and 3.90 ± 1.24. The different figures  between the two groups after remineralization shows statistical significance (p<0.01). Group B using HMU fluorinze mouthwash after 20 days did not differ from the original results (p = 0.272), in contrast with the control group (p<0.01). CONCLUSIONS: HMU fluorinze mouthwash has better mineralization effect than standard calcifying solution

Uptake capacity of metals (Al, Cu, Pb, Sn, Zn) by Vetiveria Zizanioides in contaminated water in the Dong Xam metal production trade village, Thai Binh, Vietnam

This study presents an experiment of metal contaminated water treatment under controlled environment conditions to investigate the uptake capacity of metals by Vetiveria Zizanioides to treat contaminated water from a metal production trade village, Dong Xam, Thai Binh, Vietnam. Vetiver was grown in two pot culture experiments TB10, TB6 with solutions containing respective concentrations of Al, Cu, Pb, Sn and Zn of 2.5, 55.6, 0.15, 7.7 and 24.4 mg from contaminated water in the Dong Xam metal production trade village for a period of 36 days. Vetiver has the higher tolerance to Al, Cu, Pb, Sn and Zn than other plant species. The roots (hereafter R) are high Al hyperaccumulators, concentrating 17 up to 30 folds more than “reference plant”. The upper parts of shoots (hereafter S1, S2, and S3) are 1.2 folds higher. Cu concentration in the root and shoot is up to 660 and 46.2 mg/kg, respectively. Vetiver can withstand and survive at Cu concentration of 46 mg/L in contaminated water that is markedly higher than other plants that can last only in solution with Cu concentration ranging 20-100 mg/kg. The translocation of Pb from root to shoot was 41%. Sn is more accumulated in the top, in which shoot/root ratio varied from 82% to 277% in the top, and increased to the top (by order S3/RS2/RS1/R). Zn could be translocated from roots and accumulated in shoots of vetiver. The ratio shoot/root obtains up to 46%. The present results demonstrated that vetiver had the high tolerance to trace metals Al, Cu, Pb, Sn and Zn in vegetation. This plant has a potential phytoremediation of metals in contaminated soil and wastewater from trade villages of Vietnam and other countries.References Adriano D.C., 1992. Biochemistry of trace metals. Lewis Publishers. Boca Raton, New York. 513 pp. Baker D.E., 1976. Acid soils. In Proc. of Workshop on Plant Adaptation to Mineral Stress in Problem Soils. 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Disruption of entire Cables2 locus leads to embryonic lethality by diminished Rps21 gene expression and enhanced p53 pathway

In vivo function of CDK5 and Abl enzyme substrate 2 (Cables2), belonging to the Cables protein family, is unknown. Here, we found that targeted disruption of the entire Cables2 locus (Cables2d) caused growth retardation and enhanced apoptosis at the gastrulation stage and then induced embryonic lethality in mice. Comparative transcriptome analysis revealed disruption of Cables2, 50% down-regulation of Rps21 abutting on the Cables2 locus, and up-regulation of p53-target genes in Cables2d gastrulas. We further revealed the lethality phenotype in Rps21-deleted mice and unexpectedly, the exon 1-deleted Cables2 mice survived. Interestingly, chimeric mice derived from Cables2d ESCs carrying exogenous Cables2 and tetraploid wild-type embryo overcame gastrulation. These results suggest that the diminished expression of Rps21 and the completed lack of Cables2 expression are intricately involved in the embryonic lethality via the p53 pathway. This study sheds light on the importance of Cables2 locus in mouse embryonic development.Grant-in-Aid for Scientific Research(B), Japan Society for the Promotion of Science (JSPS KAKENHI 17H03568) Fumihiro Sugiyama Grant-in-Aid for Scientific Research(S), Japan Society for the Promotion of Science (JSPS KAKENHI 26221004) Satoru Takahashi Grant-in-Aid for Scientific Research(C), Japan Society for the Promotion of Science (JSPS KAKENHI 17K07130) Hiroyoshi Iseki Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science (JSPS KAKENHI 19K16020) Tra Thi Huong Dinh Grant-in-Aid for Scientific Research(A), Japan Society for the Promotion of Science (JSPS KAKENHI 20H00444) Fumihiro Sugiyama The Cooperative Research Project Program of Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA Center), University of Tsukuba, Japan (182107) Fumihiro Sugiyam