Selection of neutralizing antibody escape mutants with type A influenza virus HA-specific polyclonal antisera: possible significance for antigenic drift

Ten antisera were produced in rabbits by two or three intravenous injections of inactivated whole influenza type A virions. All contained haemagglutination-inhibition (HI) antibody directed predominantly to an epitope in antigenic site B and, in addition, various amounts of antibodies to an epitope in site A and in site D. The ability of untreated antisera to select neutralization escape mutants was investigated by incubating virus possessing the homologous haemagglutinin with antiserum adjusted to contain anti-B epitope HI titres of 100, 1000 and 10000 HIU/ml. Virus-antiserum mixtures were inoculated into embryonated hen's eggs, and progeny virus examined without further selection. Forty percent of the antisera at a titre of 1000 HIU/ml selected neutralizing antibody escape mutants as defined by their lack of reactivity to Mab HC10 (site B), and unchanged reactivity to other Mabs to site A and site D epitopes. All escape mutant-selecting antisera had a ratio of anti-site B (HC10)-epitope antibody[ratio]other antibodies of [gt-or-equal, slanted]2·0[ratio]1. The antiserum with the highest ratio (7·4[ratio]1) selected escape mutants in all eggs tested in four different experiments. No antiserum used at a titre of 10000 HIU/ml allowed multiplication of any virus. All antisera used at a titre of 100 HIU/ml permitted virus growth, but this was wild-type (wt) virus. We conclude that a predominant epitope-specific antibody response, a titre of [gt-or-equal, slanted]1000 HIU/ml, and a low absolute titre of other antibodies ([less-than-or-eq, slant]500 HIU/ml) are three requirements for the selection of escape mutants. None of the antisera in this study could have selected escape mutants without an appropriate dilution factor, so the occurrence of an escape mutant-selecting antiserum in nature is likely to be a rare event

‘Jugglers’, ‘copers’ and ‘strugglers’: academics’ perceptions of being a head of department in a post-1992 UK university and how it influences their future careers

This study investigates the experiences of academics who became department heads in a post-1992 UK university and explores the influence that being in the position has on their planned future academic career. Drawing on life history interviews undertaken with 17 male and female heads of department, the paper constitutes an in-depth study of their careers in the same university. The findings suggest that academics who become department heads not only need the capacity to assume a range of personal and professional identities, but need flexibility to regularly adopt and switch between them. Whether individuals can successfully balance and manage such multiple identities, or whether they experience major conflicts within or between them, greatly affects their experiences of being a head of department and seems to influence their subsequent career decisions. The paper concludes by proposing a conceptual framework and typology to interpret the career trajectories of academics that became department heads in the case university

Study of guidance techniques for aerial application of agricultural compounds

Candidate systems were identified for evaluation of suitability in meeting specified accuracy requirements for a swath guidance system in an agriculture aircraft. Further examination reduced the list of potential candidates to a single category, i.e., transponder type systems, for detailed evaluation. Within this category three systems were found which met the basic accuracy requirements of the work statement. The Flying Flagman, the Electronic Flagging and the Raydist Director System. In addition to evaluating the systems against the specified requirements, each system was compared with the other two systems on a relative basis. The conclusions supported by the analyses show the Flying Flagman system to be the most suitable system currently available to meet the requirements

The Statistical Mapping of Magnetosheath Plasma Properties Based on THEMIS Measurements in the Magnetosheath Interplanetary Medium Reference Frame

The magnetosheath operates as a natural filter between the solar wind and the magnetospheric plasma. As a result of this, the magnetosheath plays a crucial role in the plasma momentum and energy transport from the interplanetary medium into the magnetosphere. Statistical studies of the magnetosheath are difficult due to the dynamic nature of the terrestrial bow shock and the magnetopause. As a result of this, the spatial and temporal dependence of magnetosheath plasma properties under varying solar wind conditions is still not completely understood. We present a study of magnetosheath plasma properties using 5 years of THEMIS and OMNI data to produce statistical maps of fundamental magnetosheath plasma properties. The magnetosheath interplanetary medium reference frame is applied to present data in a normalized reference frame which accounts for both boundary and orbital motion. The statistical maps are compared with the MHD runs from the CCMC-BATS-R-US model which agree favorably. The results are also used to investigate the presence of any magnetosheath plasma parameter asymmetries and their possible causes

The effects of fungicides on rate and duration of grain filling in winter wheat in relation to maintenance of flag leaf green area

Three field experiments were conducted on sandy-loam soils comparing the effects of triazole, strobilurin and oxazolidinedione fungicides applied at flag leaf emergence and again at ear emergence to wheat between 1998 and 2000. Cultivars Hereward and Consort were included in all 3 years, Cockpit in 1999 and 2000 and Charger in 1999. Foliar disease, green area of the flag leaf, grain weight and moisture content were assessed weekly during grain filling and senescence. Grain yield, 1000 grain weight (TGW) and specific weights were measured at harvest maturity. Septoria tritici was the dominant disease in all cultivars except Cockpit, where Puccinia striiformis caused most damage. Consort was more affected than Hereward by S. tritici in all years. Effects of fungicides on disease control were usually reflected in green flag leaf area duration (GFLAD), with large gains in Consort and Cockpit. In 2000, however, fungicides increased GFLAD of Hereward and Consort by similar amounts. Apical grains had smaller water content than medially placed grains. Maximum water content was positively influenced by fungicides where significant disease was controlled before maximum grain weight had been attained. Grain weight in Hereward was less affected than other cultivars by fungicides in both positions. Grain filling rates varied between cultivar and ear position by inconsistent and small amounts, but large and significant gains from fungicide treatment were made in grain filling periods. These varied from 0?16 days per day GFLAD in Hereward to 0?69 days per day GFLAD in Consort in apical grains in 2000. Gains in GFLAD were associated with increased yield, TGW and specific weight, but these relationships varied with cultivar. Increases in GFLAD by fungicide in Hereward in 2000 occurred much later relative to grain filling and thus after the time of maximum grain water content, resulting in smaller gains in filling duration, yield, TGW and specific weight than that seen in Consort. There was no evidence of differing relationships between GFLAD and yield for the different fungicide groups used in these experiments.authorsversionPeer reviewe

The influence of foliar diseases, and their control by fungicides, on the protein concentration in wheat grain:a review

Experiments investigating effects of foliar disease control on wheat grain protein concentration (GPC) are reviewed. Fungicidal control of rusts (Puccinia spp.) and powdery mildew (Erysiphe graminis) increased or had no significant effect on GPC in almost all cases, whilst control of the Septoria spp. often resulted in reduced GPC, but with exceptions. Reasons for these differences are discussed with reference to host pathogen interactions. Irrespective of infection strategy (biotrophic or necrotrophic), controlling severe infection increased nitrogen yield and the proportion of above-ground crop nitrogen that was partitioned to the grain. Similar effects are recorded for above-ground biomass and dry matter harvest index. The relationships between fungicide effects on green flag leaf area duration (GFLAD) and GPC were examined and shown to be unaffected by mode of action of the fungicide. Interactions between fungicide use and cultivar, nitrogen and growing season are related to the amount and type of pathogen present, and environment. An economic analysis demonstrated that fungicide effects on GPC should not affect the choice of fungicide or application programme, but that applications of foliar urea at the start of grain filling can deliver a cost-effective method of eliminating GPC reductions that may occasionally result from fungicide use.authorsversionPeer reviewe

A statistical study of magnetic field fluctuations in the dayside magnetosheath and their dependence on upstream solar wind conditions

The magnetosheath functions as a natural interface connecting the interplanetary and magnetospheric plasma. Since the magnetosheath houses the shocked solar wind, it is populated with abundant magnetic field turbulence which are generated both locally and externally. Although the steady state magnetosheath is to date relatively well understood, the same cannot be said of transient magnetic perturbations due to their kinetic nature and often complex and numerous generation mechanisms. The current manuscript presents a statistical study of magnetic field fluctuations in the dayside magnetosheath as a function of upstream solar wind conditions. We concentrate on the ambient higher-frequency fluctuations in the range of 0.1 Hz -> 2 Hz. We show evidence that the dawn (quasi-parallel) flank is visibly prone to higher-amplitude magnetic perturbations compared to the dusk (quasi-perpendicular) region. Our statistical data also suggest that the magnitude of turbulence can be visibly enhanced close to the magnetopause during periods of southward interplanetary magnetic field orientations. Faster (> 400 km s−1) solar wind velocities also appear to drive higher-amplitude perturbations compared to slower speeds. The spatial distribution also suggests some dependence on the magnetic pileup region at the subsolar magnetopause.Peer reviewe

Nonlinear Self-Organization in Photorefractive Materials

This chapter discusses self-organization and its effects in optics. One of the most exciting and potentially useful areas of current research in optics involves the understanding and exploitation of self-organization in nonlinear optical systems. This self-organization may sometimes lead to the evolution of complex spatial patterns that can be regarded as the nonlinear eigenmodes of the system. Generation of these patterns is characteristically marked by the presence of intensity thresholds. In a nonlinear system with complicated temporal dynamics, it turns out that one cannot retain purity in spatial dimensionality. It is therefore equally important to investigate the dynamics of the transverse spatial variations, which in fact give rise to very interesting patterns due to self-organization. A vast wealth of patterns can be achieved by using a nonlinear optical element with feedback that has the capability of providing field transformation, for example, by spatial filtering. These types of systems are called optical kaleidoscopes simply because of the different self-organized patterns that they can generate

Ion-Scale Wave Properties and Enhanced Ion Heating Across the Low-Latitude Boundary Layer During Kelvin-Helmholtz Instability

In the Earth\u27s magnetosphere, the magnetotail plasma sheet ions are much hotter than in the shocked solar wind. On the dawn sector, the cold-component ions are more abundant and hotter by 30–40% when compared to the dusk sector. Recent statistical studies of the flank magnetopause and magnetosheath have shown that the level of temperature asymmetry of the magnetosheath is unable to account for this, so additional physical mechanisms must be at play, either at the magnetopause or plasma sheet that contributes to this asymmetry. In this study, we perform a statistical analysis on the ion-scale wave properties in the three main plasma regimes common to flank magnetopause boundary crossings when the boundary is unstable to Kelvin-Helmholtz instability (KHI): hot and tenuous magnetospheric, cold and dense magnetosheath, and mixed (Hasegawa et al., 2004). These statistics of ion-scale wave properties are compared to observations of fast magnetosonic wave modes that have recently been linked to Kelvin-Helmholtz (KH) vortex centered ion heating (Moore et al., 2016). The statistical analysis shows that during KH events there is enhanced nonadiabatic heating calculated during ion scale wave intervals when compared to non-KH events. This suggests that during KH events there is more free energy for ion-scale wave generation, which in turn can heat ions more effectively when compared to cases when KH waves are absent. This may contribute to the dawn favored temperature asymmetry of the plasma sheet; recent studies suggest KH waves favor the dawn flank during Parker-Spiral interplanetary magnetic field

The case for early use of rapid whole-genome sequencing in management of critically ill infants: late diagnosis of Coffin-Siris syndrome in an infant with left congenital diaphragmatic hernia, congenital heart disease, and recurrent infections.

Congenital diaphragmatic hernia (CDH) results from incomplete formation of the diaphragm leading to herniation of abdominal organs into the thoracic cavity. CDH is associated with pulmonary hypoplasia, congenital heart disease, and pulmonary hypertension. Genetically, it is associated with aneuploidies, chromosomal copy-number variants, and single gene mutations. CDH is the most expensive noncardiac congenital defect. Management frequently requires implementation of extracorporeal membrane oxygenation (ECMO), which increases management expenditures 2.4-3.5-fold. The cost of management of CDH has been estimated to exceed $250 million per year. Despite in-hospital survival of 80%-90%, current management is imperfect, as a great proportion of surviving children have long-term functional deficits. We report the case of a premature infant prenatally diagnosed with CDH and congenital heart disease, who had a protracted and complicated course in the intensive care unit with multiple surgical interventions, including postcardiac surgery ECMO, gastrostomy tube placement with Nissen fundoplication, tracheostomy for respiratory failure, recurrent infections, and developmental delay. Rapid whole-genome sequencing (rWGS) identified a de novo, likely pathogenic, c.3096_ 3100delCAAAG (p.Lys1033Argfs*32) variant in ARID1B, providing a diagnosis of Coffin-Siris syndrome. Her parents elected palliative care and she died later that day