Junctional complexes and cell-cell signalling in zebrafish morphogenesis

Intercellular junctions are composed of tight, gap and adherens junctions and have been shown to play many roles in embryonic morphogenesis. I have been studying the role that intercellular junctions play in zebrafish development. First I have studies the organisation of apical neuroepithelial junctions in the developing brain. By quantifying size and segmental patterning of the junctional arrangement in the hindbrain and elsewhere I have described a level of organisation that is characteristic of compartment boundaries. I describe a distinct pattern of apical junctions in both boundary and non-boundary regions. This pattern appears to be in part regulated by the Notch signalling pathway since apical junctions distribution is different in hdac1-/- zebrafish, which have deficient Notch-Delta signalling. Second I have established that boundary cells prevent the exchange of small molecular weight, gap junction permeable dyes between adjacent CNS compartments, suggesting that the compartments are separate developmental units. Third I have studies the role of gap junction mediated intercellular communication in the propagation of calcium waves and in the coordination of cell divisions in the zebrafish blastocyst. Calcium activity is cyclical, with cells producing a greater number of calcium transients and intercellular waves during cytokinesis than during interphase. In control conditions distinct waves of cell divisions spread across the embryo in an animal to vegetal progression. I show that both the calcium activity and the cell division waves require gap junction communication because pharmacological blockade of coupling reduces the frequency of calcium activity and disrupts cell division waves

Hypernuclear structure from gamma-ray spectroscopy

The energies of p-shell hypernuclear gamma rays obtained from recent experiments using the Hyperball at BNL and KEK are used to constrain the YN interaction which enters into shell-model calculations that include both Lambda and Sigma configurations.Comment: 10 pages, 5 figures; HYP2003, Jefferson Lab, October 14-18, 2003; proceedings to appear in Nuclear Physics

Anomalous Stark Shifts in Single Vertically Coupled Pairs of InGaAs Quantum Dots

Vertically coupled Stranski Krastanow QDs are predicted to exhibit strong tunnelling interactions that lead to the formation of hybridised states. We report the results of investigations into single pairs of coupled QDs in the presence of an electric field that is able to bring individual carrier levels into resonance and to investigate the Stark shift properties of the excitons present. Pronounced changes in the Stark shift behaviour of exciton features are identified and attributed to the significant redistribution of the carrier wavefunctions as resonance between two QDs is achieved. At low electric fields coherent tunnelling between the two QD ground states is identified from the change in sign of the permanent dipole moment and dramatic increase of the electron polarisability, and at higher electric fields a distortion of the Stark shift is attributed to a coherent tunnelling effect between the ground state of the upper QD and the excited state of the lower QD.Comment: Conference paper for QD2004 3 figure

Transverse electrokinetic and microfluidic effects in micro-patterned channels: lubrication analysis for slab geometries

Off-diagonal (transverse) effects in micro-patterned geometries are predicted and analyzed within the general frame of linear response theory, relating applied presure gradient and electric field to flow and electric current. These effects could contribute to the design of pumps, mixers or flow detectors. Shape and charge density modulations are proposed as a means to obtain sizeable transverse effects, as demonstrated by focusing on simple geometries and using the lubrication approximation.Comment: 9 pages, 7 figure

The Wadi Faynan Project, Southern Jordan: a Preliminary Report on Geomorphology and Landscape Archaeology

Reproduced with permission of the publisher. © 1997 Council for British Research in the Levant. Details of the publication are available at: http://www.cbrl.org.uk/Publications/publications_default.shtmThe Wadi Faynan Project of the British Institute at Amman for Archaeology and History (BIAAH) has as its principal objective the provision of a detailed case study in the relationship between environmental change and human history in the arid zone, from prehistory to the present day. This report describes the preliminary findings of an initial campaign of fieldwork in geomorphology and landscape archaeology conducted by an inter-disciplinary team in 1996. A preliminary sequence of fluvial events has been established, represented by the Ghuwayr and Shayqar Beds dated to the Late Pleistocene, and the Faynan and Dana Beds dated to the Holocene. Methodologies have been trialed for recording, dating and interpreting the ancient field system assumed to be of Nabataean, Roman and Byzantine date; initial findings confirm its longevity of use and complexity of purpose. There are also indications that floodwater farming began in the Wadi Faynan in the Chalcolithic or Early Bronze Ag

Corn particle size and pelleting influence on growth performance, fecal shedding, and lymph node infection rates of salmonella enterica serovar typhimurium

Ninety-six pigs (initially 13.8 lb.) were used in a 28-d trial to determine the interactive effects between pelleting and particle size on Salmonella serovar Typhimurium shedding and colonization in a young growing pig model. The experiment was a 2 × 2 factorial arrangement consisting of meal or pelleted diets with fine or coarse ground corn. Pigs were fed the diets 1 wk pre-salmonella inoculation and allotted based on weight to one of four dietary treatments. For the main effect of particle size, pigs fed finer ground corn had significantly improved feed efficiency (P0.82). There was no difference in salmonella infection rates of mesenteric lymph nodes obtained on d 28 between treatments or main effects. Finer grinding and meal diets generally improved growth, feed intake, and feed efficiency compared to pigs fed coarser ground or pelleted feeds. However, particle size or diet form did not alter fecal shedding or mesenteric lymph node infection rates of salmonella organisms in our study

Automated Certification of Authorisation Policy Resistance

Attribute-based Access Control (ABAC) extends traditional Access Control by considering an access request as a set of pairs attribute name-value, making it particularly useful in the context of open and distributed systems, where security relevant information can be collected from different sources. However, ABAC enables attribute hiding attacks, allowing an attacker to gain some access by withholding information. In this paper, we first introduce the notion of policy resistance to attribute hiding attacks. We then propose the tool ATRAP (Automatic Term Rewriting for Authorisation Policies), based on the recent formal ABAC language PTaCL, which first automatically searches for resistance counter-examples using Maude, and then automatically searches for an Isabelle proof of resistance. We illustrate our approach with two simple examples of policies and propose an evaluation of ATRAP performances.Comment: 20 pages, 4 figures, version including proofs of the paper that will be presented at ESORICS 201

Childhood IQ and cardiovascular disease in adulthood: prospective observational study linking the Scottish Mental Survey 1932 and the Midspan studies

This study investigated the influence of childhood IQ on the relationships between risk factors and cardiovascular disease (CVD), coronary heart disease (CHD) and stroke in adulthood. Participants were from the Midspan prospective cohort studies which were conducted on adults in Scotland in the 1970s. Data on risk factors were collected from a questionnaire and at a screening examination, and participants were followed up for 25 years for hospital admissions and mortality. 938 Midspan participants were successfully matched with their age 11 IQ from the Scottish Mental Survey 1932, in which 1921-born children attending schools in Scotland took a cognitive ability test. Childhood IQ was negatively correlated with diastolic and systolic blood pressure, and positively correlated with height and respiratory function in adulthood. For each of CVD, CHD and stroke, defined as either a hospital admission or death, there was an increased relative rate per standard deviation decrease (15 points) in childhood IQ of 1.11 (95% confidence interval 1.01-1.23), 1.16 (1.03-1.32) and 1.10 (0.88-1.36) respectively. With events divided into those first occurring before and those first occurring after the age of 65, the relationships between childhood IQ and CVD, CHD and stroke were only seen before age 65 and not after age 65. Blood pressure, height, respiratory function and smoking were associated with CVD events. Relationships were stronger in the early compared to the later period for smoking and FEV1, and stronger in the later compared to the earlier period for blood pressure. Adjustment for childhood IQ had small attenuating effects on the risk factor-CVD relationship before age 65 and no effects after age 65. Adjustment for risk factors attenuated the childhood IQ-CVD relationship by a small amount before age 65. Childhood IQ was associated with CVD risk factors and events and can be considered an important new risk factor

Height and risk of death among men and women: aetiological implications of associations with cardiorespiratory disease and cancer mortality

OBJECTIVES: Height is inversely associated with cardiovascular disease mortality risk and has shown variable associations with cancer incidence and mortality. The interpretation of findings from previous studies has been constrained by data limitations. Associations between height and specific causes of death were investigated in a large general population cohort of men and women from the West of Scotland. DESIGN: Prospective observational study. SETTING: Renfrew and Paisley, in the West of Scotland. SUBJECTS: 7052 men and 8354 women aged 45-64 were recruited into a study in Renfrew and Paisley, in the West of Scotland, between 1972 and 1976. Detailed assessments of cardiovascular disease risk factors, morbidity and socioeconomic circumstances were made at baseline. MAIN OUTCOME MEASURES: Deaths during 20 years of follow up classified into specific causes. RESULTS: Over the follow up period 3347 men and 2638 women died. Height is inversely associated with all cause, coronary heart disease, stroke, and respiratory disease mortality among men and women. Adjustment for socioeconomic position and cardiovascular risk factors had little influence on these associations. Height is strongly associated with forced expiratory volume in one second (FEV1) and adjustment for FEV1 considerably attenuated the association between height and cardiorespiratory mortality. Smoking related cancer mortality is not associated with height. The risk of deaths from cancer unrelated to smoking tended to increase with height, particularly for haematopoietic, colorectal and prostate cancers. Stomach cancer mortality was inversely associated with height. Adjustment for socioeconomic position had little influence on these associations. CONCLUSION: Height serves partly as an indicator of socioeconomic circumstances and nutritional status in childhood and this may underlie the inverse associations between height and adulthood cardiorespiratory mortality. Much of the association between height and cardiorespiratory mortality was accounted for by lung function, which is also partly determined by exposures acting in childhood. The inverse association between height and stomach cancer mortality probably reflects Helicobacter pylori infection in childhood resulting inor being associated withshorter height. The positive associations between height and several cancers unrelated to smoking could reflect the influence of calorie intake during childhood on the risk of these cancers

Searching for the Slater Transition in the Pyrochlore Cd2_{2}Os2_{2}O7_{7} with Infrared Spectroscopy

Infrared reflectance measurements were made on the single crystal pyrochlore Cd$_{2}$Os$_{2}$O$_{7}$ in order to examine the transformations of the electronic structure and crystal lattice across the boundary of the metal insulator transition at $T_{MIT}=226K$. All predicted IR active phonons are observed in the conductivity over all temperatures and the oscillator strength is found to be temperature independent. These results indicate that charge ordering plays only a minor role in the MIT and that the transition is strictly electronic in nature. The conductivity shows the clear opening of a gap with $2\Delta=5.2k_{B}T_{MIT}$. The gap opens continuously, with a temperature dependence similar to that of BCS superconductors, and the gap edge having a distinct $\sigma(\omega)\thicksim\omega^{1/2}$ dependence. All of these observables support the suggestion of a Slater transition in Cd$_{2}$Os$_{2}$O$_{7}$.Comment: 4 pages, 4 figure