Nanomotion technology in combination with machine learning: a new approach for a rapid antibiotic susceptibility test for Mycobacterium tuberculosis.

Nanomotion technology is a growth-independent approach that can be used to detect and record the vibrations of bacteria attached to cantilevers. We have developed a nanomotion-based antibiotic susceptibility test (AST) protocol for Mycobacterium tuberculosis (MTB). The protocol was used to predict strain phenotype towards isoniazid (INH) and rifampicin (RIF) using a leave-one-out cross-validation (LOOCV) and machine learning techniques. This MTB-nanomotion protocol takes 21 h, including cell suspension preparation, optimized bacterial attachment to functionalized cantilever, and nanomotion recording before and after antibiotic exposure. We applied this protocol to MTB isolates (n = 40) and were able to discriminate between susceptible and resistant strains for INH and RIF with a maximum sensitivity of 97.4% and 100%, respectively, and a maximum specificity of 100% for both antibiotics when considering each nanomotion recording to be a distinct experiment. Grouping recordings as triplicates based on source isolate improved sensitivity and specificity to 100% for both antibiotics. Nanomotion technology can potentially reduce time-to-result significantly compared to the days and weeks currently needed for current phenotypic ASTs for MTB. It can further be extended to other anti-TB drugs to help guide more effective TB treatment

National identity and environmentalism: why national narcissism might undermine pro-environmental efforts

The extant literature recognizes national identity as a pivotal factor motivating both individual and collective actions to tackle environmental problems. Yet, prior research shows mixed evidence for the relationship between national identity and environmentalism. Here, we propose a theoretical approach that articulates distinctions between different forms of national identity and their differential associations with environmental attitudes and behaviors. Specifically, we argue that it is key to differentiate national identification, which reflects a positive attachment to one's country and ties to other compatriots, from national narcissism, which reflects viewing one's country as exceptional and deserving of special treatment. In contrast to national identification, national narcissism is consistently associated with lower environmental concern and predicts support for anti-environmental policies. We show that this is likely due to national narcissism being linked to belief in climate-related conspiracy theories, support for policies that challenge external pressures yet present the nation in a positive light (e.g., greenwashing), and focusing on short-term benefits for the nation. Extending past individual-level findings, we report a pre-registered analysis across 56 countries examining whether national narcissism is also linked to objective indices of lower environmental protection at the country level of analysis. Results revealed a negative relationship between countries' environmental performance and country-level national narcissism (while adjusting for national identification and GDP per capita). We discuss the theoretical and practical implications of our approach and the country-level findings for advancing research in the field

Understanding defensive and secure in-group positivity: The role of collective narcissism

Integrating psychoanalytic ideas of group idealisation with social identity and categorisation theories, this article discusses the distinction between secure and defensive in-group positivity. Narcissistic in-group positivity captures a belief in in-group greatness that is contingent on external validation. It reflects defensive in-group positivity, insofar as it stems from the frustration of individual needs, and predicts increased sensitivity to threats as well as undesirable consequences for out-groups and the in-group. Secure in-group positivity—that is, in-group positivity without the narcissistic component—is a confidently held positive evaluation of one’s in-group that is independent of the recognition of the group in the eyes of others. It stems from the satisfaction of individual needs, is resilient to threats and has positive consequences for the in-group and out-groups. I review evidence for these two distinct ways people relate to their social groups and discuss theoretical and practical implications for understanding intra- and intergroup relations

Support for collective action against refugees: The role of national, European, and global identifications, and autochthony beliefs

To understand recent anti-refugee protests in Europe, we examined how different levels of inclusiveness of group identities (national, European, and global) are related to intentions to protest among native Europeans. We focused on the mediating role of autochthony (a belief that the first inhabitants of a territory are more entitled) and the moderating role of threat. Survey data from 11 European countries (N=1909) showed that national identification was positively associated with autochthony, and therefore, with the intention to protest against refugees. In contrast, global identification was related to lower protest intentions via lower autochthony. These paths were found only among Europeans who perceived refugees as a threat. European identification was not related to the endorsement of autochthony or to collective action. These findings indicate why and when majority members are willing to participate in collective action against refugees, and underscore the importance of global identification in the acceptance of refugees

β -delayed neutron emission from Ga 85

Decay of Ga85 was studied by means of β-neutron-γ spectroscopy. A pure beam of Ga85 was produced at the Holifield Radioactive Ion Beam Facility using a resonance ionization laser ion source and a high-resolution electromagnetic separator. The β-delayed neutron emission probability was measured for the first time, yielding 70(5)%. An upper limit of 0.1% for β-delayed two-neutron emission was also experimentally established for the first time. A detailed decay scheme including absolute γ-ray intensities was obtained. Results are compared with theoretical β-delayed emission models

Excited states in As 82 studied in the decay of Ge 82

The excited states of odd-odd As82 are studied in the β decay of Ge82. An isotopically pure beam of Ga83 was produced at the Holifield Radioactive Ion Beam Facility using a resonance ionization laser ion source and high-resolution electromagnetic separation. The atoms of Ge82 are created after β-delayed neutron emission in the decay of Ga83. The number of Ge82 atoms is found by normalization to the 1348-keV γ ray. Detailed analysis of the decay scheme is compared with shell-model calculations with several commonly used fpg shell interactions