Cadmium and arsenic-induced-stress differentially modulates Arabidopsis root architecture, peroxisome distribution, enzymatic activities and their nitric oxide content

In plant cells, cadmium (Cd) and arsenic (As) exert toxicity mainly by inducing oxidative stress through an imbalance between the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS), and their detoxification. Nitric oxide (NO) is a RNS acting as signalling molecule coordinating plant development and stress responses, but also as oxidative stress inducer, depending on its cellular concentration. Peroxisomes are versatile organelles involved in plant metabolism and signalling, with a role in cellular redox balance thanks to their antioxidant enzymes, and their RNS (mainly NO) and ROS. This study analysed Cd or As effects on peroxisomes, and NO production and distribution in the root system, including primary root (PR) and lateral roots (LRs). Arabidopsis thaliana wild-type and transgenic plants enabling peroxisomes to be visualized in vivo, through the expression of the 35S-cyan fluorescent protein fused to the peroxisomal targeting signal1 (PTS1) were used. Peroxisomal enzymatic activities including the antioxidant catalase, the H2O2-generating glycolate oxidase, and the hydroxypyruvate reductase, and root system morphology were also evaluated under Cd/As exposure. Results showed that Cd and As differently modulate these activities, however, catalase activity was inhibited by both. Moreover, Arabidopsis root system was altered, with the pollutants differently affecting PR growth, but similarly enhancing LR formation. Only in the PR apex, and not in LR one, Cd more than As caused significant changes in peroxisome distribution, size, and in peroxisomal NO content. By contrast, neither pollutant caused significant changes in peroxisomes size and peroxisomal NO content in the LR apex

Highlights from the Third International Society for Computational Biology Student Council Symposium at the Fifteenth Annual International Conference on Intelligent Systems for Molecular Biology

RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.Abstract In this meeting report we give an overview of the 3rd International Society for Computational Biology Student Council Symposium. Furthermore, we explain the role of the Student Council and the symposium series in the context of large, international conferences.Published versio

DNAContentViewer a BioJS component to visualise GC/AT Content

Summary: Compositional GC/AT content of DNA sequences is a useful feature in genome analysis. GC/AT content provides useful information about evolution, structure and function of genomes, giving clues about their biological function and organisation. We have developed DNAContentViewer, a BioJS component for visualisation of compositional GC/AT content in raw sequences. DNAContentViewer has been integrated in the BioJS project as part of the BioJS registry of components. DNAContentViewer requires a simple configuration and installation. Its design allows potential interactions with other components via predefined events

BDAFRICA: Design and implementation of a database of Postcolonial African literature published in Spain

Este trabajo demuestra que no existe un repositorio que incluya los autores poscoloniales africanos publicados hasta el momento en España y que permita, por ende, realizar estudios cuantitativos y cualitativos del impacto de esta literatura con la precisión deseable. Esto supone una carencia tanto para investigaciones académicas como para el sector editorial a la hora de analizar tendencias de selección y recepción en el mercado. Ante esta situación, el objetivo primordial de este trabajo es diseñar e implementar una base de datos, basada en MySQL y delimitada por unos parámetros muy concretos, que recoja todas las obras de autores africanos publicadas en castellano en España entre 1972 (año en que España se unió al sistema ISBN) y 2014. Tras determinar unos criterios de diseño y unos protocolos de compilación específcos, el desarrollo metodológico se ha dividido en cuatro fases: recopilación, almacenamiento, tratamiento y difusión de los datos. Así, la base de datos BDÁFRICA consigue un doble objetivo: por un lado, proporciona a los investigadores datos fables en los que basar sus estudios y, por otro, permitiría ofrecer por primera vez datos estadísticos de la evolución de la publicación de obras de autores africanos en España en los últimos 42 años.English version of abstract: This paper evidences the absence of a repository or database that scompiles the publications of Postcolonial African writing in Spain, thus precluding comprehensive quantitative or qualitative studies on the impact of these works. This is a significant obstacle for both academic research and the book market, which up to now has been unable to analyse market selection and reception trends. To cater for this need, the aim of this paper is to describe the design and implementation of a relational database, based on MySQL and set up using specific and accurate parameters, that includes all the literary works penned by African writers and published in Spain and in Spanish from 1972 (when Spain first joined the ISBN system) to 2014. After determining a number of specific design criteria and compilation protocols, the methodology has been divided into four steps: data collection, storage, statistical processing and online dissemination. Thus, the database BDAFRICA achieves a twofold objective: firstly, it provides researchers with unbiased data as a framework for their studies, and secondly, it offers unprecedented statistics on the evolution of the publication of African literature in Spain over the last 42 years.Published versio

The reception in Spain of African literature written in English: Statistical data generation using the specialised bibliographic database BDÁFRICA

El presente artículo examina la recepción de la literatura africana en lengua inglesa en España basándonos en BDÁFRICA, una base de datos bibliográfica que recoge obras de autores nacidos en África y publicadas en español y en España entre 1972 y 2014. Se ofrece una reflexión crítica de las dificultades para definir la literatura africana como objeto de estudio, debido a su complejidad y heterogeneidad. Se propone, además, un conciso recorrido historiográfico por la conformación del canon de dicha literatura que se ha realizado desde Occidente. Asimismo, se demuestra la falta de estudios estadísticos sobre la recepción de literatura africana en lengua inglesa en España. Respondiendo a esta necesidad, el objetivo del artículo es detallar y analizar los datos estadísticos inéditos que proporciona la base de datos, adoptando una metodología descriptiva. Los resultados de este estudio, que aporta datos cuantitativos y cualitativos fiables y novedosos, son originales en tanto en cuanto reflejan y señalan los problemas de la traducción de la literatura africana en lengua inglesa en España. BDÁFRICA, que es gratuita y está disponible en red, pretende ser un recurso y una fuente que estimule el desarrollo de la investigación en literatura poscolonial en España. Sin duda, esta base de datos bibliográfica especializada es una herramienta muy valiosa, especialmente para investigadores, traductores y editoriales interesados en literatura africana.English translation of abstract: This paper explores the reception in Spain of African literature written originally in English by focusing on BDÁFRICA, a bibliographical database of works of fiction by African-born authors published in Spain, in Spanish between 1972 and 2014. It provides a critical reflection on the constraints of defining the object of study when African literature is discussed, due to the complexity and heterogeneity of such literature. It also proposes a concise historiographical journey through the Western canon formation of African literature in English. Additionally, it evidences the lack of quantitative studies on the reception in Spain of African literature in English. To cater to this need, the aim of this paper is to report and analyse the groundbreaking statistical data the database itself provides by following a descriptive approach. The findings of this study, which provides reliable and unprecedented quantitative and qualitative data, are original in the sense that they map and highlight the problems of the reception of African literature in English, and translated in Spain. This shows BDÁFRICA, which is free and available online, as a primary (re)source for work to pave the way for the development of postcolonial literature research in Spain. This specialised bibliographical database is undoubtedly an invaluable tool, especially for researchers, translators and publishing houses interested in African literature.Published versio

Whole Genome Interpretation for a Family of Five.

Although best practices have emerged on how to analyse and interpret personal genomes, the utility of whole genome screening remains underdeveloped. A large amount of information can be gathered from various types of analyses via whole genome sequencing including pathogenicity screening, genetic risk scoring, fitness, nutrition, and pharmacogenomic analysis. We recognize different levels of confidence when assessing the validity of genetic markers and apply rigorous standards for evaluation of phenotype associations. We illustrate the application of this approach on a family of five. By applying analyses of whole genomes from different methodological perspectives, we are able to build a more comprehensive picture to assist decision making in preventative healthcare and well-being management. Our interpretation and reporting outputs provide input for a clinician to develop a healthcare plan for the individual, based on genetic and other healthcare data

Comparing post-editing difficulty of different machine translation errors in Spanish and German translations from English

Post-editing (PE) of Machine Translation (MT) is an increasingly popular way to integrate MT in the professional translation workflow, as it increases productivity and income. However, the quality of MT is not always good enough to blindly choose PE over translation from scratch. This article studies the PE of different error types and compares indicators of PE difficulty in English-to-Spanish and English-to-German translations. The results show that the indicators in question 1) do not correlate between each other for all error types, and 2) differ between languages

Crowdsourcing the corpasome

The suffix -ome conveys “comprehensiveness” in some way. The idea of the Corpasome started half-jokingly, acknowledging the efforts to sequence five members of my family. After the unexpected response from many scientists from around the world, it has become clear how useful this approach could be for understanding the genomic information contained in our personal genomics tests