Polar Gateways Arctic Circle Sunrise 2008 Conference at the Top of the World

The Polar Gateways conference was hosted during January 23-29, 2008, the first week of polar sunrise at Barrow, Alaska, at the new Barrow Arctic Research Center of the Barrow Arctic Science consortium (BASC). The dawn week of polar day, the highly variable low temperatures, and the ice-covered shore tundra and adjacent sea ice conditions provided an appropriate locale for a conference dedicated in the spirit of the International Polar and Heliophysical Years 2007-2009 to the educational exploration of polar and icy world science of Earth and the solar system. The many scientific, educational, and cultural interactions with the local community of four thousand residents, sixty percent native Inupiat Eskimo, further provided an unforgettable experience of what life might be someday be like on other remote polar and icy worlds to be explored and eventually inhabited. Over one hundred active participants, more than half participating remotely, contributed science presentations and educational activities during this unique circumpolar and very "green" conference. Most remote contributions came via videoconference from the Swedish Institute of Space Physics (IRF) at Kisuna, Sweden, the EISCAT Svalbard Radar Facility at Spitzbergen, Norway, the University of Alaska at Fairbanks, NASA Goddard Space Flight Center and the Jet Propulsion Laboratory, the University of California at Berkeley, and the University of Arizona. A few contributors participated via teleconference, including one from the Polar Geophysical Institute at Apatity in Russia. These active contributions spanned up to thirteen time zones (Alaska to Russia) at various tirnes during the conference. Primary videoconferencing support between Barrow and other sites was ably provided by the University of Alaska at Fairbanks, and local operators at each remote site collectively made this conference possible. Science presentations spanned the solar system from the polar Sun and heliospheric environment to Earth, Moon, Mars, Jupiter, Saturn, the Kuiper Belt, and the solar wind termination shock now crossed by both Voyager spacecraft. Barrow participants experienced look and feel of icy worlds like Europa by going "on the ice" during snowmobile expeditions to the near-shore sea ice and Point Barrow. Extensive educational outreach activities were conducted with the local Barrow township and North Slope Borough communities, partly through several interviews with local host Earl Finkler on Barrow's KBRW Radio, and through the NASA Digital Learning Network (DLN) "live from the top of the world" at Barrow. The Goddard robotic rover "Nunuq of the North" became a local celebrity. The complete science program and photo library, eventually also including video recordings of all main presentations, will be available at the new polargateways2008.gsfc.nasa.gov web site (old version: polargateways2008.org) with links to educational materials from the conference already accessible at sunearthday.nasa.gov/polarsunrise

Transforming growth factor-β: activation by neuraminidase and role in highly pathogenic H5N1 influenza pathogenesis.

Transforming growth factor-beta (TGF-β), a multifunctional cytokine regulating several immunologic processes, is expressed by virtually all cells as a biologically inactive molecule termed latent TGF-β (LTGF-β). We have previously shown that TGF-β activity increases during influenza virus infection in mice and suggested that the neuraminidase (NA) protein mediates this activation. In the current study, we determined the mechanism of activation of LTGF-β by NA from the influenza virus A/Gray Teal/Australia/2/1979 by mobility shift and enzyme inhibition assays. We also investigated whether exogenous TGF-β administered via a replication-deficient adenovirus vector provides protection from H5N1 influenza pathogenesis and whether depletion of TGF-β during virus infection increases morbidity in mice. We found that both the influenza and bacterial NA activate LTGF-β by removing sialic acid motifs from LTGF-β, each NA being specific for the sialic acid linkages cleaved. Further, NA likely activates LTGF-β primarily via its enzymatic activity, but proteases might also play a role in this process. Several influenza A virus subtypes (H1N1, H1N2, H3N2, H5N9, H6N1, and H7N3) except the highly pathogenic H5N1 strains activated LTGF-β in vitro and in vivo. Addition of exogenous TGF-β to H5N1 influenza virus-infected mice delayed mortality and reduced viral titers whereas neutralization of TGF-β during H5N1 and pandemic 2009 H1N1 infection increased morbidity. Together, these data show that microbe-associated NAs can directly activate LTGF-β and that TGF-β plays a pivotal role protecting the host from influenza pathogenesis

Curriculum Guidelines for Graduate and Undergraduate Virology Courses

Curriculum guidelines for virology are needed to best guide student learning due to the continuous and ever-increasing volume of virology information, the need to ensure that undergraduate and graduate students have a foundational understanding of key virology concepts, and the importance in being able to communicate that understanding to both other virologists and nonvirologists. Such guidelines, developed by virology educators and the American Society for Virology Education and Career Development Committee, are described herein

Spatiotemporal changes in influenza A virus prevalence among wild waterfowl inhabiting the continental United States throughout the annual cycle.

Avian influenza viruses can pose serious risks to agricultural production, human health, and wildlife. An understanding of viruses in wild reservoir species across time and space is important to informing surveillance programs, risk models, and potential population impacts for vulnerable species. Although it is recognized that influenza A virus prevalence peaks in reservoir waterfowl in late summer through autumn, temporal and spatial variation across species has not been fully characterized. We combined two large influenza databases for North America and applied spatiotemporal models to explore patterns in prevalence throughout the annual cycle and across the continental United States for 30 waterfowl species. Peaks in prevalence in late summer through autumn were pronounced for dabbling ducks in the genera Anas and Spatula, but not Mareca. Spatially, areas of high prevalence appeared to be related to regional duck density, with highest predicted prevalence found across the upper Midwest during early fall, though further study is needed. We documented elevated prevalence in late winter and early spring, particularly in the Mississippi Alluvial Valley. Our results suggest that spatiotemporal variation in prevalence outside autumn staging areas may also represent a dynamic parameter to be considered in IAV ecology and associated risks

Assessing the fitness of distinct clades of influenza A (H9N2) viruses

Influenza A (H9N2) viruses are a genetically diverse population that infects wild and domestic avian species and mammals and contributed the internal gene segments to the A/H5N1 and A/H7N9 viruses associated with lethal human infections. Here we comprehensively assess the potential risk to mammals of a diverse panel of A/H9N2 viruses, representing the major H9N2 clades, using a combination of in vitro assays (e.g., antiviral susceptibility and virus growth in primary differentiated human airway cells) and in vivo assays (e. g., replication, transmission and/or pathogenicity of viruses in ducks, pigs, mice and ferrets). We observed that viruses isolated from humans, A/Hong Kong/1073/1999 and A/Hong Kong/33982/2009, had the highest risk potential. However, the A/swine/Hong Kong/9A-1/1998 and A/chicken/Hong Kong/G9/1997 viruses also displayed several features suggesting a fitness profile adapted to human infection and transmission. The North American avian H9N2 clade virus had the lowest risk profile, and the other viruses tested displayed various levels of fitness across individual assays. In many cases, the known genotypic polymorphisms alone were not sufficient to accurately predict the virus' phenotype. Therefore, we conclude that comprehensive risk analyses based on surveillance of circulating influenza virus strains are necessary to assess the potential for human infection by emerging influenza A viruses