Building envelope design as a contribution for improvement of urban spaces and social housing environmental quality

The design of building envelope and the definition of its elements, can influence both the quality of the external spaces perception and the living standard referred to internal building spaces. This improvement depends by the planning of some component design. Particularly, solar shadings and integrated plant solutions, also thanks to an increasing consequential interest about the issue and the legislative and normative evolution, represent factors able to be involved both in the performance and morphological quality of building envelope (improvement of energy efficiency and living quality of internal spaces), which can influence the perception of environment. A study about this questions has been conducted through the elaboration of a system of Best Pratices, a Code of Practice, for the new Plans of Zone of Rome Municipality. The indications contained in the Code takes in examination the integration-mitigation and facilities connection of solar collectors in the building design, and the possibility of integration between solar shading and collector elements, customized like a support tool for the sustainable design of building envelope. The design of building envelope, reported to morphological and technological issues, can assume particular importance in the definition of living quality. Design of closures, developed through some indications referred to its technological components, can influence both the quality perception of external living spaces through the morphological definition of building, and the life quality of internal spaces by the implementation of energy efficiency of building system. Solar shading in particular, also thanks to the increasing consequential interest in the evolution of legislation about the argument, more in the future will represent a fundamental element for design and the increment of performance and morphological quality of building enclosure

Annual Ryegrass Pasture for Dairy Cows Receiving Total Mixed Ration

The inclusion of herbage in the diet of medium yielding dairy cows offered a total mixed ration (TMR) may be beneficial. This study, which involved mid lactation dairy cows, examined the effect of partial replacement of a TMR with annual temperate pasture. Treatments were ad libitum TMR (TMR100), 75% ad libitum TMR + ryegrass (Lolium multiflorum ‘Maximus’) (TMR75), and 50% ad libitum TMR + ryegrass (TMR50). Twelve multiparous Holstein and F1 Jersey × Holstein cows were divided into six homogeneous groups, taking account of milk production (26.6 ± 4.5 kg/day), days in milk (128 ± 50) and body weight (546 ± 31 kg). Treatments were compared in a replicated 3×3 Latin square design, comprising three 21-day periods (measurements during final 5 days). Cows on TMR75 and TMR50 strip grazed between morning and afternoon milking (7 h/day), with a target pre- and post-grazing sward height of 24 and 12 cm, respectively. Herbage DM intake was estimated as the difference between pre- and post-grazing herbage mass. The TMR and herbage had a crude protein content of 150 and 303 g/kg DM, and a NDF content of 366 and 495 g/kg DM, respectively. Herbage DM intake increased from 4.8 kg/day in TMR75 to 6.7 kg/day on TMR50. Total DM intake decreased from 19.4 kg/day (TMR100), to 18.1 and 15.9 kg/day (TMR75 and TMR50, respectively). Milk production, milk fat and milk protein content were similar between treatments, averaging 25.6 kg/day, 44.6 g/kg and 33.7 g/kg, respectively. The net energy for lactation (NEL) supply was 113, 104 and 92% of NEL requirements, for cows receiving TMR100, TMR75 and TMR50, respectively. Ryegrass pastures were able to replace up to 50% of TMR offered to mid lactation dairy cows without any adverse effects on milk production and milk composition

A common deep source for upper-mantle upwellings below the Ibero-western Maghreb region from teleseismic P-wave travel-time tomography

Upper-mantle upwellings are often invoked as the cause of Cenozoic volcanism in the Ibero-western Maghreb region. However, their nature, geometry and origin are unclear. This study takes advantage of dense seismic networks, which cover an area extending from the Pyrenees in the north to the Canaries in the south, to provide a new high-resolution P-wave velocity model of the upper-mantle and topmost lower-mantle structure. Our images show three subvertical upper-mantle upwellings below the Canaries, the Atlas Ranges and the Gibraltar Arc, which appear to be rooted beneath the upper-mantle transition zone (MTZ). Two other mantle upwellings beneath the eastern Rif and eastern Betics surround the Gibraltar subduction zone. We propose a new geodynamic model in which narrow upper-mantle upwellings below the Canaries, the Atlas Ranges and the Gibraltar Arc rise from a laterally-propagating layer of material below the MTZ, which in turn is fed by a common deep source below the Canaries. In the Gibraltar region, the deeply rooted upwelling interacts with the Gibraltar slab. Quasi-toroidal flow driven by slab rollback induces the hot mantle material to flow around the slab, creating the two low-velocity anomalies below the eastern Betics and eastern Rif. Our results suggest that the Central Atlantic plume is a likely source of hot mantle material for upper-mantle upwellings in the Ibero-western Maghreb region

Smart solutions for low-income buildings rehabilitation: international researches and experiences

[EN] Smart City concept briefly refers to a sustainable city where innovative Smart strategies will be adopted to an efficient management of resources flows and social interoperability. Aligned with the most relevant European research experiences, the paper describes the relations between an on-going research from the PDTA Dept., centered on a knowledge platform tool for energy-efficient interactive buildings design, and the contribution of the ELIH-MED project on the use of industrialized solutions and smart devices during the rehabilitation process in Mediterranean low-income housings. A pilot project in the Spanish climate context reveals the important role of Smart Monitoring Devices to encourage energy savings and tackle recognized needs through a participative process where stakeholders and beneficiaries are actively involved.[IT] Il termine Smart City può essere sinteticamente associato al concetto di città sostenibile dove strategie innovative intelligenti favoriscono la gestione efficiente del flusso di risorse e l' interoperabilità sociale. Con riferimento alle più significative esperienze di ricerca europee, il contributo approfondisce i legami tra una ricerca, attualmente in corso presso il Dipartimento PDTA, rivolta allo sviluppo di una piattaforma per la progettazione di edifici interattivi ed energeticamente efficienti, e il progetto ELIH-MED incentrato sull'uso di soluzioni industrializzate e dispositivi Smart nell¿ambito di interventi di riqualificazione di edifici sociali nel bacino del Mediterraneo. Un progetto pilota situato nel contesto climatico spagnolo mostra l'impatto derivante dall¿uso di soluzioni Smart nel favorire il risparmio energetico e rispondere alle esigenze degli utenti attraverso un processo partecipativo che coinvolge attivamente stakeholder e beneficiari.The ELIH-Med project represents a significant pilot experience that has allowed to identify and demonstrate, through pilot-scale actions, the feasibil-ity of efficient and innovative solutions and innovative financial mechanisms, backed by ERDF (European Regional Development Fund) funds.Arbizzani. E.; Civiero, P.; Ortega Madrigal, L.; Serrano Lanzarote, AB. (2015). Soluzioni Smart per la riqualificazione degli edifici residenziali sociali: ricerche ed esperienze internazionali. Techne: journal of technology for architecture and environment. 10:222-231. https://doi.org/10.13128/Techne-17519S2222311

Relationships between energy balance during early lactation and cow performance, blood metabolites, and fertility: A meta-analysis of individual cow data

Publication history: Accepted - 15 January 2021; Published online - 6 March 2021This study was designed to contribute to the understanding of the relationships between energy balance (EB) in early lactation [4 to 21 d in milk (DIM)] and fertility traits [interval to start of luteal activity (SLA), interval to first observed heat (FOH), and conception to first artificial insemination (AI)], and their associated relationships with cow performance and blood metabolites between 4 to 150 DIM. Individual cow data (488 primiparous and 1,020 multiparous lactations) from 27 experiments was analyzed. Data on cow performance, EB (on a metabolizable energy basis), and fertility traits were available for all cows, whereas milk progesterone data (to determine SLA) and periodic blood metabolite data were available for 1,042 and 1,055 lactations, respectively. Data from primiparous and multiparous cows were analyzed separately, with the data sets for the 2 parity groups divided into quartiles (Q1–Q4) according to the average EB during 4 to 21 DIM (EB range for Q1 to Q4: primiparous, −120 to −49, −49 to −24, −24 to −3, and −3 to 92 MJ/d, respectively: multiparous, −191 to −79, −79 to −48, −48 to −22, and −22 to 93 MJ/d, respectively). Differences between EB quartiles for production and fertility traits were compared. In early lactation (4 to 21 DIM), moving from Q1 to Q4 mean DMI and metabolizable energy intake increased whereas mean ECM decreased. During the same period, moving from Q1 to Q4 milk fat content, milk fat-to-protein ratio, and plasma nonesterified fatty acid and β-hydroxybutyrate concentrations decreased, whereas milk protein content and plasma glucose concentrations increased in both primiparous and multiparous cows. When examined over the entire experimental period (4 to 150 DIM), many of the trends in intakes and milk production remained, although the magnitude of the difference between quartiles was much reduced, whereas milk fat content did not differ between quartiles in primiparous cows. The percentage of cows with FOH before 42 DIM increased from Q1 to Q4 (from 46 to 72% in primiparous cows, and from 41 to 58% in multiparous cows). Interval from calving to SLA and to FOH decreased with increasing EB during 4 to 21 DIM, with these occurring 9.8 and 10.2 d earlier, respectively, in Q4 compared with Q1 (primiparous cows), and 7.4 and 5.9 d earlier, respectively, in Q4 compared with Q1 (multiparous cows). For each 10 MJ/d decrease in mean EB during 4 to 21 DIM, FOH was delayed by 1.2 and 0.8 d in primiparous and multiparous cows, respectively. However, neither days to first AI nor the percentage of cows that conceived to first AI were affected by daily EB during 4 to 21 DIM in either primiparous or multiparous cows, and this is likely to reflect a return to a less metabolically stressed status at the time of AI. These results demonstrate that interval from calving to SLA and to FOH were reduced with increasing EB in early lactation, whereas early lactation EB had no effect on conception to the first service.This work was supported by the Department of Agriculture and Rural Development for Northern Ireland (DAERA, Northern Ireland, UK) as part of the Department of Agriculture, Food, and the Marine (DAFM, Republic of Ireland) NutriGen project. Maurício Civiero was in receipt of a scholarship from Programa de Bolsas Universitárias de Santa Catarina (UNIEDU/FUMDES, Florianópolis, SC, Brazil) and thanks Centro Universitário Vale do Iguaçu (UNIGUAÇU, União da Vitória, PR, Brazil) for providing time to complete this wo

PAK6 Phosphorylates 14-3-3 gamma to Regulate Steady State Phosphorylation of LRRK2

Mutations in Leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson’s disease (PD) and, as such, LRRK2 is considered a promising therapeutic target for age-related neurodegeneration. Although the cellular functions of LRRK2 in health and disease are incompletely understood, robust evidence indicates that PD-associated mutations alter LRRK2 kinase and GTPase activities with consequent deregulation of the downstream signaling pathways. We have previously demonstrated that one LRRK2 binding partner is P21 (RAC1) Activated Kinase 6 (PAK6). Here, we interrogate the PAK6 interactome and find that PAK6 binds a subset of 14-3-3 proteins in a kinase dependent manner. Furthermore, PAK6 efficiently phosphorylates 14-3-3γ at Ser59 and this phosphorylation serves as a switch to dissociate the chaperone from client proteins including LRRK2, a well-established 14-3-3 binding partner. We found that 14-3-3γ phosphorylated by PAK6 is no longer competent to bind LRRK2 at phospho-Ser935, causing LRRK2 dephosphorylation. To address whether these interactions are relevant in a neuronal context, we demonstrate that a constitutively active form of PAK6 rescues the G2019S LRRK2-associated neurite shortening through phosphorylation of 14-3-3γ. Our results identify PAK6 as the kinase for 14-3-3γ and reveal a novel regulatory mechanism of 14-3-3/LRRK2 complex in the brain

Thermal Nature of Mantle Upwellings Below the Ibero-Western Maghreb Region Inferred From Teleseismic Tomography

©2019. American Geophysical Union. All Rights Reserved. Independent models of P wave and S wave velocity anomalies in the mantle derived from seismic tomography help to distinguish thermal signatures from those of partial melt, volatiles, and compositional variations. Here we use seismic data from SW Europe and NW Africa, spanning the region between the Pyrenees and the Canaries, in order to obtain a new S-SKS relative arrival-time tomographic model of the upper mantle below Iberia, Western Morocco, and the Canaries. Similar to previous P wave tomographic results, the S wave model provides evidence for (1) subvertical upper-mantle low-velocity structures below the Canaries, Atlas Ranges, and Gibraltar Arc, which are interpreted as mantle upwellings fed by a common lower-mantle source below the Canaries; and (2) two low-velocity anomalies below the eastern Rif and Betics that we interpret as the result of the interaction between quasi-toroidal mantle flow induced by the Gibraltar slab and the mantle upwelling behind it. The analysis of teleseismic P wave and S wave arrival-time residuals and the conversion of the low-velocity anomalies to temperature variations suggest that the upwellings in the upper mantle below the Canaries, Atlas Ranges, and Gibraltar Arc system may be solely thermal in nature, with temperature excesses in the range ~100–350 °C. Our results also indicate that local partial melting can be present at lithospheric depths, especially below the Atlas Ranges. The locations of thermal mantle upwellings are in good agreement with those of thinned lithosphere, moderate to high heat-flow measurements, and recent magmatic activity at the surface

The LRRK2 signalling system

The LRRK2 gene is a major contributor to genetic risk for Parkinson's disease and understanding the biology of the leucine-rich repeat kinase 2 (LRRK2, the protein product of this gene) is an important goal in Parkinson's research. LRRK2 is a multi-domain, multi-activity enzyme and has been implicated in a wide range of signalling events within the cell. Because of the complexities of the signal transduction pathways in which LRRK2 is involved, it has been challenging to generate a clear idea as to how mutations and disease associated variants in this gene are altered in disease. Understanding the events in which LRRK2 is involved at a systems level is therefore critical to fully understand the biology and pathobiology of this protein and is the subject of this review

LRRK2 Biology from structure to dysfunction: research progresses, but the themes remain the same

Since the discovery of leucine-rich repeat kinase 2 (LRRK2) as a protein that is likely central to the aetiology of Parkinson's disease, a considerable amount of work has gone into uncovering its basic cellular function. This effort has led to the implication of LRRK2 in a bewildering range of cell biological processes and pathways, and probable roles in a number of seemingly unrelated medical conditions. In this review we summarise current knowledge of the basic biochemistry and cellular function of LRRK2. Topics covered include the identification of phosphorylation substrates of LRRK2 kinase activity, in particular Rab proteins, and advances in understanding the activation of LRRK2 kinase activity via dimerisation and association with membranes, especially via interaction with Rab29. We also discuss biochemical studies that shed light on the complex LRRK2 GTPase activity, evidence of roles for LRRK2 in a range of cell signalling pathways that are likely cell type specific, and studies linking LRRK2 to the cell biology of organelles. The latter includes the involvement of LRRK2 in autophagy, endocytosis, and processes at the trans-Golgi network, the endoplasmic reticulum and also key microtubule-based cellular structures. We further propose a mechanism linking LRRK2 dimerisation, GTPase function and membrane recruitment with LRRK2 kinase activation by Rab29. Together these data paint a picture of a research field that in many ways is moving forward with great momentum, but in other ways has not changed fundamentally. Many key advances have been made, but very often they seem to lead back to the same places