3 research outputs found
Genome Sequence of the Native Apiculate Wine Yeast Hanseniaspora vineae T02/19AF
The use of novel yeast strains for winemaking improves quality and provides variety including subtle characteristic differences in fine wines. Here we report the first genome of a yeast strain native to Uruguay, Hanseniaspora vineae T02/19AF, which has been shown to positively contribute to aroma and wine quality.Fil: Giorello, Facundo M.. Universidad de la República; UruguayFil: Berná, Luisa. Instituto Pasteur de Montevideo; UruguayFil: Greif, Gonzalo. Instituto Pasteur de Montevideo; UruguayFil: Camesasca, Laura. Inst. de Investigaciones Biológicas Clemente Estable; UruguayFil: Salzman, Valentina. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Medina, Karina. Universidad de la Republica. Facultad de QuÃmica; UruguayFil: Robello, Carlos. Instituto Pasteur de Montevideo; UruguayFil: Gaggero, Carina. Inst. de Investigaciones Biológicas Clemente Estable; UruguayFil: Aguilar, Pablo S.. Instituto Pasteur de Montevideo; UruguayFil: Carrau, Francisco. Sección EnologÃa; Urugua
The role of Saccharomyces cerevisiae in the production of floral aromas in wines by monoterpene biosynthesis
<i>De Novo</i> Synthesis of Benzenoid Compounds by the Yeast <i>Hanseniaspora vineae</i> Increases the Flavor Diversity of Wines
Benzyl alcohol and
other benzenoid-derived metabolites of particular
importance in plants confer floral and fruity flavors to wines. Among
the volatile aroma components in <i>Vitis vinifera</i> grape
varieties, benzyl alcohol is present in its free and glycosylated
forms. These compounds are considered to originate from grapes only
and not from fermentative processes. We have found increased levels
of benzyl alcohol in red Tannat wine compared to that in grape juice,
suggesting <i>de novo</i> formation of this metabolite during
vinification. In this work, we show that benzyl alcohol, benzaldehyde, <i>p</i>-hydroxybenzaldehyde, and <i>p</i>-hydroxybenzyl
alcohol are synthesized <i>de novo</i> in the absence of
grape-derived precursors by <i>Hanseniaspora vineae</i>.
Levels of benzyl alcohol produced by 11 different <i>H. vineae</i> strains were 20–200 times higher than those measured in fermentations
with <i>Saccharomyces cerevisiae</i> strains. These results
show that <i>H. vineae</i> contributes to flavor diversity
by increasing grape variety aroma concentration in a chemically defined
medium. Feeding experiments with phenylalanine, tryptophan, tyrosine, <i>p</i>-aminobenzoic acid, and ammonium in an artificial medium
were tested to evaluate the effect of these compounds either as precursors
or as potential pathway regulators for the formation of benzenoid-derived
aromas. Genomic analysis shows that the phenylalanine ammonia-lyase
(<i>PAL</i>) and tyrosine ammonia lyase (<i>TAL</i>) pathways, used by plants to generate benzyl alcohols from aromatic
amino acids, are absent in the <i>H. vineae</i> genome.
Consequently, alternative pathways derived from chorismate with mandelate
as an intermediate are discussed