1,495 research outputs found

    A Student of the Old Testament Proclaims Jesus as Messiah and Lord

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    This paper proposes to examine the stance of Peter both in his position toward the Old Testament which he quotes and toward the people before him on Pentecost. It is the contention of this writer that Peter was a man with both feet in the world. Moreover, Peter\u27s world consisted of two separate but often merging spheres of life. There is the orb of the past, full of history and tradition, but there is also the sphere of a living present with its dynamic reality in the living Christ and His Spirit

    Tissue Compartmentalization and Tropism of HIV-1: A Dissertation

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    Despite the development of effective antiretroviral treatments, there is still no cure for HIV-1. Major barriers to HIV-1 eradication include the diversity of intrapatient viral quasispecies and the establishment of reservoirs in tissue sanctuary sites. A better understanding of these populations is required for targeted treatments. While previous studies have examined the relationship between brain and blood or immune tissues, few have looked at and compared the properties of viruses from other tissue compartments. In this study, 75 full length HIV-1 envelopes were isolated from the frontal lobe, occipital lobe, parietal lobe, colon, lung, and lymph node of an HIV-1 infected subject. No envelopes could be amplified from the plasma or serum. Envelopes were subjected to genotypic and phenotypic characterization. Of the 75 envelopes, 53 were able to infect HeLa TZM-bl cells. The greatest proportion of non-functional envelopes was from the lung, a result of APOBEC-induced hypermutation. Lower frequencies of hypermutation were also observed in the occipital lobe and colon. Envelopes from regions of the brain were almost all macrophage tropic, while those from the body were predominantly non-macrophage tropic. All envelopes used CCR5 as a coreceptor. Phylogenetic analyses showed that sequences were compartmentalized inside the brain. These findings were also observed using PacBio next generation sequencing to examine 32,152 full length sequences. Envelopes from tissues of the body displayed greater variation in sequence length, charge, and number of potential N-linked glycosylation sites in comparison to envelopes from tissues of the brain. Increased variation was also observed in IC50s for inhibition and neutralization assays using sCD4, maraviroc, b12, PG16, 17b, and 447-52D. The increased variation observed in envelopes from tissues outside the brain suggests that different pressures may be influencing the evolution of these viruses and emphasizes the importance of further studies in these tissue sites

    The development and utilization of EST (Expressed Sequence Tag) resources in the diploid strawberry model system

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    Enhancement of genomic resources is needed for the cultivated octoploid strawberry Fragaria x ananassa (2n=8x=56). A diploid (2n=2x=14) relative, Fragaria vesca, has been used as a model system for strawberry genomics because of its small (~200 Mb) basic genome size, ancestry to the octoploid strawberries and availability of inbred lines, among other favorable features. In this study, a cDNA library was constructed from developing flower buds of Fragaria vesca ssp. vesca cv. \u27Yellow Wonder\u27. A cDNA library of 7680 clones was created using the CLONTECH SMART cDNA Library Construction kit. Sequencing and bioinformatic analyses of 3298 clones resulted in 2717 quality Expressed Sequence Tags (ESTs) and 1910 unigenes. ESTs appear in GenBank as accession numbers DV438013--DV440729. Of these sequences, approximately 600 did not have homology to sequences in any of the databases used for sequence similarity searches. (Abstract shortened by UMI.)

    Gas Sensing Properties of Metal Oxide Nanostructured Heterojunctions

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    Engineering: 2nd Place (The Ohio State University Denman Undergraduate Research Forum)Titanium dioxide (TiO2) has demonstrated great potential for resistive-type gas sensors used to detect hydrocarbons and volatile organic compounds (VOC). Two parameters which affect the sensitivity of gas sensors is the surface area of the sensing material and interfacial potentials. This experiment is exploring the VOC selectivity and gas-sensing ability of TiO2 aerogel, the highest surface area TiO2 – based material, and metal oxide powders (MOX) containing single crystalline TiO2 nanorods. Nanorods are high surface area structures which alter the potential at surface interfaces. The nanorod structures are hydrothermally grown directly onto the powders with the use of a microwave synthesis system. These hybrid powders are then used to fabricate sensors which are tested in ethanol and acetone saturated air. Thus far, commercial grade TiO2, SnO2, NiO, and CoO powders and TiO2 aerogel have been used for sensor fabrication. Initial results indicate that the response of aerogel-based sensors is highly dependent upon the quality of the aerogel. Preliminary tests have also shown that the presence of nanorods appreciably increases the response of the sensors to ethanol and acetone saturated air. The SnO2 hybrid (containing nanorods) and CoO hybrid sensors show promise for be selective towards ethanol. Selectivity towards ethanol or acetone would allow the sensor to be used as a tool for preemptive diagnoses of diseases, such as diabetes. It has been found that patients with certain diseases possess elevated concentrations of specific VOC’s in their breath. There has only been limited success in developing a sensor which is sensitive to either acetone or ethanol, two of the main VOC’s used for diagnosis. The relationship between a sensor’s response, selectivity, and surface interface composition is being analyzed. Future work includes analyzing the relationship between temperature and response and fabricating sensors made of mixed MOX powders, instead of hydrothermal compositing.The Ohio State University, Department of Materials Science and EngineeringAcademic Major: Materials Science and Engineerin

    The Vermont Safe Infant Sleep Project: Evaluation of an Online Learning Module

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    The Vermont Safe Infant Sleep Project: Evaluation of an Online Learning Module Brigid J. Meehan-Brese DNPc, RN Rosemary Dale Ed.D, APRN, Julie Parent MSW, and Jean Pelski PhD, APRN, NNP-BC Abstract Background: In the United States, sudden unintended infant death syndrome (SUIDS) is a leading cause of infant mortality. Accidental suffocation, and strangulation in bed (ASSB) deaths are the third leading cause of postnatal deaths (CDC, 2021). The Vermont Safe Infant Sleep Project is a collaborative effort focused on standardizing evidence-based practice recommendations for nurses to support safe infant sleep practices. An online learning module for registered nurses was developed in phase one of the project. Purpose: In phase two, the online learning module was evaluated for efficacy and ease of use by registered nurses and maternal-child educators caring for infants across the state of Vermont. Methods: Participants completed the online learning module including a pre- and post-test and survey. The quantitative data were collected and analyzed using a web-based test, allowing for a direct question-to-question comparison. The qualitative data were collected using an online survey assessing reception and ease of use. Results: 30 participants from two regional hospitals and a state agency completed pre-tests, 25 finished post-tests, and 5 completed post-test surveys. Combined averages for the registered nurses and maternal-child educators pre-test and post-test scores were 14.00 or 77% and 16.4 or 91% representing a 17% improvement overall. 100% of survey participants found the course to be convenient and user-friendly. Conclusions: The module is effective in closing knowledge gaps. Participants improved their scores after the module. Surveys indicated that the module is user-friendly and convenient. The data indicates a positive reception to the module. Keywords: Infant mortality; sudden infant death syndrome; Accidental infant suffocation; Accidental infant strangulation; Infant sleep practice; Adult learning principles Reference CDC, C. D. C. (2021, April 28). Sudden Unexpected Infant Death and Sudden Infant Death Syndrome: Data and Statistics. Centers for Disease Control and Prevention. Retrieved September 26, 2021, from https://www.cdc.gov/sids/data.htm

    Tetra­aqua­bis(biuret-κ2 O,O′)yttrium(III) trichloride

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    In the title compound, [Y(C2H5N3O2)2(H2O)4]Cl3, the Y3+ ion (site symmetry 2) is bonded to eight O atoms (arising from two O,O′-bidentate biuret mol­ecules and four water mol­ecules) in a distorted square-anti­prismatic arrangement. A network of N—H⋯O, N—H⋯Cl and O—H⋯Cl hydrogen bonds help to establish the packing, leading to a three-dimensional network. One of the chloride ions has site symmetry 2

    Crystal structure of the pyrochlore oxide superconductor KOs2_2O6_6

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    We report the single-crystal X-ray analysis of the structure of the pyrochlore oxide superconductor KOs2_2O6_6. The structure was identified as the β\beta-pyrochlore structure with space group Fd3ˉmFd\bar{3}m and lattice constant aa = 10.089(2)~\AA at 300 K: the K atom is located at the 8bb site, not at the 16dd site as in conventional pyrochlore oxides. We found an anomalously large atomic displacement parameter UisoU_\mathrm{iso} = 0.0735(8)~\AA2^2 at 300 K for the K cation, which suggests that the K cation weakly bound to an oversized Os12_{12}O18_{18} cage exhibits intensive rattling, as recently observed for clathrate compounds. The rattling of A cations is a common feature in the series of β\beta-pyrochlore oxide superconductors AOs2_2O6_6 (A = Cs, Rb and K), and is greatest for the smallest K cation.Comment: 5 pages, 5 figures, to appear in J. Solid. State. Che

    Tetra­aqua­bis(biuret-κ2 O,O′)gadolinium(III) trichloride

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    In the title compound, [Gd(C2H5N3O2)2(H2O)4]Cl3, which is isostrucutural with its yttrium analogue, the Gd3+ ion (site symmetry 2) is bonded to eight O atoms (arising from two O,O′-bidentate biuret mol­ecules and four water mol­ecules) in a distorted square-anti­prismatic arrangement. A network of N—H⋯O, N—H⋯Cl and O—H⋯Cl hydrogen bonds helps to establish the packing, leading to a three-dimensional network. One of the chloride ions has site symmetry 2

    Monolanthanum tripotassium tetra­hydrogen deca­molybdodicobaltate(III) trideca­hydrate

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    The title compound, K3La[H4Mo10Co2O38].13H2O, is an optically active chiral polyoxometalate (POM) which contains an anion with ideal point symmetry D2 (222). The crystals containing one of the enanti­omer pairs in the POM were resolved at pH 2.5. The factor that governs the formation of the compound is the pH condition of the mother liquor. The racemate salt, K6[H4Mo10Co2O38]·7H2O, is obtained at pH 6.5 [Nolan et al. (1998 ▶). Aust. J. Chem. 51. 825–834]. Two non-acidic H atoms in the POM form intra­molecular hydrogen bonds and the remaining two H atoms form hydrogen bonds with two water mol­ecules. The POMs are connected by three K+ ions. The La3+ ion is coordinated by three O atoms of the POM and six water molecues with distances in the range 2.516 (5)–2.589 (5) Å

    Assessing Student Knowledge, Background and Perceptions in the International Civic and Citizenship Study

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    It is over the ten years since IEA last investigated civic education, and in that time new challenges have emerged in educating young people for their roles as citizens in the 21st century. These challenges have stimulated renewed reflection on the meanings of citizenship and the roles of and approaches to civic and citizenship education. In many countries there is a growing interest in using evidence to improve policy and practice in civic and citizenship education. ICCS is the third international IEA study in this area and it is explicitly linked through common questions to the IEA Civic Education Study (CIVED) which was undertaken in 1999 and 2000 (Torney-Purta, Lehmann, Oswald and Schulz, 2001; Amadeo et. al., 2004; Schulz and Sibberns, 2004). The study will survey 13-to-14-year old students in 38 countries in the years 2008 and 2009 and report on student achievement and perceptions related to civic and citizenship education. Outcome data will be obtained from representative samples of students in their eighth year of schooling and context data from the students, their schools and teachers as well as through national centres. The study builds on the previous IEA study of civic education (CIVED) undertaken in 1999. Information about ICCS can be found at http://iccs.acer.edu.au/ .1 It is recognised that there is substantial diversity in the field of civic and citizenship education within and across countries. Consequently, maximising the involvement of researchers from participating countries in this international comparative study is deemed to be of particular importance for the success of this study. Input from national research centres is sought throughout the study and the consortium has developed strategies that have maximised country contributions to the instrument development for the field trial and will continue to involve countries in the selection process of item material for main survey
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