Lack of association between dietary fructose and hyperuricemia risk in adults

<p>Abstract</p> <p>Background</p> <p>High serum uric acid concentration (hyperuricemia) has been studied for its relationship with multiple adverse health outcomes, such as metabolic syndrome. Intervention studies have produced inconsistent outcomes for the relationship between fructose intake and serum uric acid concentration.</p> <p>Methods</p> <p>The association of dietary fructose intake with hyperuricemia risk in adults was examined using logistic regression and U.S. NHANES 1999-2004 databases. A total of 9,384 subjects, between the ages 20 and 80 years, without diabetes, cancer, or heart disease, were included.</p> <p>Results</p> <p>The highest added or total fructose intake (quartiles by grams or % energy) was not associated with an increase of hyperuricemia risk compared to the lowest intake with or without adjustment (odds ratios = 0.515-0.992). The associations of alcohol and fiber intakes with the risk were also determined. Compared to the lowest intake, the highest alcohol intake was associated with increased mean serum uric acid concentration (up to 16%, <it>P </it>< 0.001) and hyperuricemia risk (odds ratios = 1.658-1.829, <it>P </it>= 0.057- < 0.001); the highest fiber intake was correlated with decreases of uric acid concentration (up to 7.5%, <it>P </it>< 0.002) and lower risk (odds ratios = 0.448-0.478, <it>P </it>= 0.001- < 0.001). Adults who were over 50 y old, male, or obese had significantly greater risk.</p> <p>Conclusions</p> <p>The data show that increased dietary fructose intake was not associated with increased hyperuricemia risk; while increased dietary alcohol intake was significantly associated with increased hyperuricemia risk; and increased fiber intake was significantly associated with decreased hyperuricemia risk. These data further suggest a potential effect of fructose consumption in an ordinary diet on serum uric acid differs from results found in some short-term studies using atypical exposure and/or levels of fructose administration.</p

The structure of cyclic fatty acid monomers isolated from heated linseed oil and their effects on cultured aortic endothelial cells

This study reports the complete structural configuration of CFAM isolated from heated linseed oil and the influence of these components in cultured porcine aortic endothelial cells. Using CFAM-oxazoline derivatives, location of unsaturation and ring structures were confirmed using gas chromatography-matrix isolation-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The CFAM were identified as 18-carbon diunsaturated structures containing cyclopentenyl and cyclohexenyl rings in various positions along the molecule. One site of cis unsaturation was always within the ring structure while the other site of unsaturation was either cis or trans and could be located at various positions in either the carboxyl- or methyl-terminal chain of the ring. Three pairs were identified as cyclic stereoisomers.After incubation with CFAM-containing media for 48 hours, CFAM were incorporated into the membrane phospholipids of cultured endothelial cells. No decrease in endothelial cell density or viability was obsessed. A significant decrease in the activity of calcium ATPase as well as a significant decrease in the integrity of the endothelial cell monolayer were observed following incubation with CFAM-containing media. A decrease in the activity of total ATPase also appeared to be caused by incubation with CFAM.The incubation of CFAM-containing media caused an increase in the fluidity of the membrane hydrocarbon region as measured by the steady-state anisotropy of DPH. This observed fluidity change may be an artifact caused by DPH incorporation into the intracellular lipid droplets present in CFAM-treated cells. The measurements of the steady-state anisotropy of both TMA-DPH and DPH-PA showed no change in the membrane fluidity.Endothelial cell growth was shown to be influenced by the presence of fatty-acid free bovine serum albumin, which was used to introduce CFAM into the culture media, and not due to the incorporation of CFAM into endothelial cell membranes. Prostacyclin production was significantly increased in endothelial cells due to incubation with CFAM-BSA compared to BSA alone. The presence of CFAM was either counteracting the inhibitory influence of BSA on PGI\sb2 production or directly stimulating PGI\sb2 secretion by cultured endothelial cells.U of I OnlyETDs are only available to UIUC Users without author permissio