Determinants of the population growth of the West Nile virus mosquito vector Culex pipiens in a repeatedly affected area in Italy

Background The recent spread of West Nile Virus in temperate countries has raised concern. Predicting the likelihood of transmission is crucial to ascertain the threat to Public and Veterinary Health. However, accurate models of West Nile Virus (WNV) expansion in Europe may be hampered by limited understanding of the population dynamics of their primary mosquito vectors and their response to environmental changes.<p></p> Methods We used data collected in north-eastern Italy (2009–2011) to analyze the determinants of the population growth rate of the primary WNV vector Culex pipiens. A series of alternative growth models were fitted to longitudinal data on mosquito abundance to evaluate the strength of evidence for regulation by intrinsic density-dependent and/or extrinsic environmental factors. Model-averaging algorithms were then used to estimate the relative importance of intrinsic and extrinsic variables in describing the variations of per-capita growth rates.<p></p> Results Results indicate a much greater contribution of density-dependence in regulating vector population growth rates than of any environmental factor on its own. Analysis of an average model of Cx. pipiens growth revealed that the most significant predictors of their population dynamics was the length of daylight, estimated population size and temperature conditions in the 15 day period prior to sampling. Other extrinsic variables (including measures of precipitation, number of rainy days, and humidity) had only a minor influence on Cx. pipiens growth rates.<p></p> Conclusions These results indicate the need to incorporate density dependence in combination with key environmental factors for robust prediction of Cx. pipiens population expansion and WNV transmission risk. We hypothesize that detailed analysis of the determinants of mosquito vector growth rate as conducted here can help identify when and where an increase in vector population size and associated WNV transmission risk should be expected.<p></p&gt

Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond

More than 40 years ago, Howard Green's laboratory developed a method for long-term expansion of primary human epidermal keratinocytes by co-culture with 3T3 mouse embryonic fibroblasts. This was a breakthrough for in vitro cultivation of cells from human skin and later for other epithelia: it led to the first stem cell therapy using cultured cells and has vastly increased our understanding of epithelial stem cell biology. In recent years, new methods to expand epithelial cells as three-dimensional organoids have provided novel means to investigate the functions of these cells in health and disease. Here, we outline the history of stratified epithelial stem cell culture and the application of cultured epithelial cells in clinical therapies. We further discuss the derivation of organoids from other types of epithelia and the challenges that remain for the translation of novel stem cell therapies toward clinical use

Current and Future Therapeutic Approaches for Thymic Stromal Cell Defects

Inborn errors of thymic stromal cell development and function lead to impaired T-cell development resulting in a susceptibility to opportunistic infections and autoimmunity. In their most severe form, congenital athymia, these disorders are life-threatening if left untreated. Athymia is rare and is typically associated with complete DiGeorge syndrome, which has multiple genetic and environmental etiologies. It is also found in rare cases of T-cell lymphopenia due to Nude SCID and Otofaciocervical Syndrome type 2, or in the context of genetically undefined defects. This group of disorders cannot be corrected by hematopoietic stem cell transplantation, but upon timely recognition as thymic defects, can successfully be treated by thymus transplantation using cultured postnatal thymic tissue with the generation of naïve T-cells showing a diverse repertoire. Mortality after this treatment usually occurs before immune reconstitution and is mainly associated with infections most often acquired pre-transplantation. In this review, we will discuss the current approaches to the diagnosis and management of thymic stromal cell defects, in particular those resulting in athymia. We will discuss the impact of the expanding implementation of newborn screening for T-cell lymphopenia, in combination with next generation sequencing, as well as the role of novel diagnostic tools distinguishing between hematopoietic and thymic stromal cell defects in facilitating the early consideration for thymus transplantation of an increasing number of patients and disorders. Immune reconstitution after the current treatment is usually incomplete with relatively common inflammatory and autoimmune complications, emphasizing the importance for improving strategies for thymus replacement therapy by optimizing the current use of postnatal thymus tissue and developing new approaches using engineered thymus tissue

Experimental investigation of the mooring system of a wave energy converter in operating and extreme wave conditions

A proper design of the mooring systems for Wave Energy Converters (WECs) requires an accurate investigation of both operating and extreme wave conditions. A careful analysis of these systems is required to design a mooring configuration that ensures station keeping, reliability, maintainability, and low costs, without affecting the WEC dynamics. In this context, an experimental campaign on a 1:20 scaled prototype of the ISWEC (Inertial Sea Wave Energy Converter), focusing on the influence of the mooring layout on loads in extreme wave conditions, is presented and discussed. Two mooring configurations composed of multiple slack catenaries with sub-surface buoys, with or without clump-weights, have been designed and investigated experimentally. Tests in regular, irregular, and extreme waves for a moored model of the ISWEC device have been performed at the University of Naples Federico II. The aim is to identify a mooring solution that could guarantee both correct operation of the device and load carrying in extreme sea conditions. Pitch motion and loads in the rotational joint have been considered as indicators of the device hydrodynamic behavior and mooring configuration impact on the WEC

An X-ray Survey of Galaxies in Pairs

Results are reported from the first survey of X-ray emission from galaxies in pairs. The sample consists of fifty-two pairs of galaxies from the Catalog of Paired Galaxies Karachentsev (1972) whose coordinates overlap ROSAT Position Sensitive Proportional Counter pointed observations. The mean observed log l_x for early-type pairs is 41.35 +/-0.21 while the mean log l_x predicted using the l_x-l_b relationship for isolated early-type galaxies is 42.10 +/-0.19. With 95% confidence, the galaxies in pairs are underluminous in the X-ray, compared to isolated galaxies, for the same l_b. A significant fraction of the mixed pair sample also appear similarly underluminous. A spatial analysis shows that the X-ray emission from pairs of both types typically has an extent of ~10 - 50 kpc, much smaller than group intergalactic medium and thus likely originates from the galaxies. CPG 564, the most X-ray luminous early-type pair, 4.7x10^42 ergs/sec, is an exception. The extent of it's X-ray emission, >169 kpc, and HWHM, ~80 kpc, is comparable to that expected from an intergalactic medium. The sample shows only a weak correlation, ~81% confidence, between l_x and l_b, presumably due to variations in gas content within the galaxies. No correlation between l_x and the pair velocity difference, separation, or far-infrared luminosity is found though the detection rate is low, 22%.Comment: 40 pages, 6 jpg figures, ApJ (in press

Geochemistry of fluids discharged over the seismic area of the Southern Apennines (Calabria region, Southern Italy): Implications for Fluid-Fault relationships

The first comprehensive geochemical data-set of the fluids circulating over a 14,000 km2-wide seismicprone area of the Southern Apennines, Calabria Region (Italy), is presented here. The geochemical investigations were carried out with the twofold aim of constraining the origin and interactions of the circulating fluids and to investigate possible relationships with local faults. Sixty samples of both thermal and cold waters were collected, from which the dissolved gases were extracted. The geochemical features of the water samples display different types and degrees of water–rock interactions, irrespective of the outlet temperature. The calculated equilibrium temperatures of the thermal waters (60–160 C) and the low heat flow of thewhole study area, are consistent with a heating process due to deep water circulation and rapid upflow through lithospheric structures. The composition of the dissolved gases reveals that crustal-originating gases (N2 and CO2-dominated) feed all the groundwaters. The 3He/4He ratios of the dissolved He, in the range of 0.03–0.22Rac for the thermal waters and 0.05–0.63Rac for the cold waters (Rac = He isotope ratio corrected for atmospheric contamination), are mainly the result of a two-component (radiogenic and atmospheric) mixing, although indications of mantle-derived He are found in some cold waters. As the study area had been hit by 18 of the most destructive earthquakes (magnitude ranging from 5.9 to 7.2) occurring over a 280-a time span (1626–1908) in the Southern Apennines, the reported results on the circulating fluids may represent the reference for a better inside knowledge of the fault-fluid relationships and for the development of long-term geochemical monitoring strategies for the area

Analysis and Design of a 1.8-GHz CMOS LC Quadrature VCO

This paper presents a quadrature voltage-controlled oscillator (QVCO) based on the coupling of two LC-tank VCOs. A simplified theoretical analysis for the oscillation frequency and phase noise displayed by the QVCO in the 1/f/sup 3/ region is developed, and good agreement is found between theory and simulation results. A prototype for the QVCO was implemented in a 0.35-/spl mu/m CMOS process with three standard metal layers. The QVCO could be tuned between 1.64 and 1.97 GHz, and showed a phase noise of -140 dBc/Hz or less across the tuning range at a 3-MHz offset frequency from the carrier, for a current consumption of 25 mA from a 2-V power supply. The equivalent phase error between I and Q signals was at most 0.25/spl deg/

Integrating formal methods into medical software development : the ASM approach

Medical devices are safety-critical systems since their malfunctions can seriously compromise human safety. Correct operation of a medical device depends upon the controlling software, whose development should adhere to certification standards. However, these standards provide general descriptions of common software engineering activities without any indication regarding particular methods and techniques to assure safety and reliability. This paper discusses how to integrate the use of a formal approach into the current normative for the medical software development. The rigorous process is based on the Abstract State Machine (ASM) formal method, its refinement principle, and model analysis approaches the method supports. The hemodialysis machine case study is used to show how the ASM-based design process covers most of the engineering activities required by the related standards, and provides rigorous approaches for medical software validation and verification

Optical spectroscopy of galaxies in the direction of the Virgo cluster

Optical spectroscopy of 76 galaxies, 48 of which are projected in the direction of the Virgo cluster and 28 onto the Coma-A1367 supercluster, is reported. Adding these new measurements to those found in the literature, the redshift completeness in the Virgo region becomes 92% at B_T<=16.0 and 68% at B_T<=18.0. The one of CGCG galaxies in the direction of the Coma-A1367 supercluster becomes 98%. The Virgo cluster membership estimates obtained on morphological grounds by Binggeli et al. (1985) are confirmed in all cases. However, several "possible members" classified as BCD (if in the cluster) are found instead to be giant emission-line galaxies in the background of the Virgo clusterComment: LaTeX, 8 pages, 5 figures, accepted for pubblication in A&

Multi-configurational Ehrenfest simulations of ultrafast nonadiabatic dynamics in a charge-transfer complex

Multi-configurational Ehrenfest (MCE) approaches, which are intended to remedy the lack of correlations in the standard mean-field Ehrenfest method, have been proposed as coherent-state based ansatze for quantum propagation [D. V. Shalashilin, J. Chem. Phys. 130, 244101 (2009)] and also as the classical limit of the variational Gaussian-based multiconfiguration time dependent Hartree (G-MCTDH) method [S. Romer and I. Burghardt, Mol. Phys. 111, 3618 (2013)]. In the present paper, we establish the formal connection between these schemes and assess the performance of MCE for a coherent-state representation of the classical-limit subsystem. As a representative model system, we address the ultrafast, coherent charge transfer dynamics in an oligothiophene-fullerene donor acceptor complex described by a two-state linear vibronic coupling model. MCE calculations are compared with reference calculations performed with the MC IDH method, for 10-40 vibrational modes. Beyond a dimensionality of 10 modes, it is shown that the correct representation of electronic coherence depends crucially on the sampling of initially unoccupied Gaussians