FEDERALISMO Y FEDERACIÓN EN FRANCIA: ¿HISTORIA DE UN CONCEPTO IMPENSABLE?

Se diría imposible hacer la historia de los conceptos de federalismo y federación en Francia, pues la experiencia política francesa parece contradecir íntegramente el espíritu del federalismo. ¿Estamos en presencia de una «historia prohibida», o de la historia de un concepto impensable en Francia? ¿Acaso debemos contentarnos con hacer una historia negativa, en el vacío, por tanto, y con elaborar la lista de todas las acusaciones lanzadas contra el federalismo que ha producido la doctrina político-jurídica francesa durante los dos últimos siglos? Esta conferencia, entonces, podría presentarse como la historia de una cita malograda entre el pensamiento político-jurídico francés y el federalismo

Identification of sites phosphorylated by the vaccinia virus B1R kinase in viral protein H5R

Background: Vaccinia virus gene B1R encodes a erine/threonine protein kinase. In vitro this protein kinase phosphorylates ribosomal proteins Sa and S2 and vaccinia virus protein H5R, proteins that become phosphorylated during infection. Nothing is known about the sites phosphorylated on these proteins or the general substrate specificity of the kinase. The work described is the first to address these questions. Results: Vaccinia virus protein H5R was phosphorylated by the B1R protein kinase in vitro, digested with V8 protease, and phosphopeptides separated by HPLC. The N-terminal sequence of one radioactively labelled phosphopeptide was determined and found to correspond to residues 81-87 of the protein, with Thr-84 and Thr-85 being phosphorylated. A synthetic peptide based on this region of the protein was shown to be a substrate for the B1R protein kinase, and the extent of phosphorylation was substantially decreased if either Thr residue was replaced by an Ala. Conclusions: We have identified the first phosphorylation site for the vaccinia virus B1R protein kinase. This gives important information about the substrate-specificity of the enzyme, which differs from that of other known protein kinases. It remains to be seen whether the same site is phosphorylated in vivo

Using Stories and Statistics to Advocate for Support

With resources at a premium, great attention is being paid to persuasive methods and convincing stakeholders to support and advocate for archival and library needs. Digital libraries, like others, are not immune to the seemingly perpetual budget cuts across our field. However, using stories and statistics to illustrate our impact can help advocate for our needs and preserve our digital history. This session will focus on compiling qualitative stories from our patrons with quantifiable statistics to advocate for digital preservation needs. Using Digital Collections at the University of Southern Mississippi as a case study, this session will show examples of patron stories and measurable statistics to advocate for a digital preservation system and larger data preservation needs at the university. While the example is academic, the overall framework is adaptable to all settings