Trypanosoma cruzi Gene Expression in Response to Gamma Radiation

Trypanosoma cruzi is an organism highly resistant to ionizing radiation. Following a dose of 500 Gy of gamma radiation, the fragmented genomic DNA is gradually reconstructed and the pattern of chromosomal bands is restored in less than 48 hours. Cell growth arrests after irradiation but, while DNA is completely fragmented, RNA maintains its integrity. In this work we compared the transcriptional profiles of irradiated and non-irradiated epimastigotes at different time points after irradiation using microarray. In total, 273 genes were differentially expressed; from these, 160 were up-regulated and 113 down-regulated. We found that genes with predicted functions are the most prevalent in the down-regulated gene category. Translation and protein metabolic processes, as well as generation of precursor of metabolites and energy pathways were affected. In contrast, the up-regulated category was mainly composed of obsolete sequences (which included some genes of the kinetoplast DNA), genes coding for hypothetical proteins, and Retrotransposon Hot Spot genes. Finally, the tyrosyl-DNA phosphodiesterase 1, a gene involved in double-strand DNA break repair process, was up-regulated. Our study demonstrated the peculiar response to ionizing radiation, raising questions about how this organism changes its gene expression to manage such a harmful stress

The Short Non-Coding Transcriptome of the Protozoan Parasite Trypanosoma cruzi

The pathway for RNA interference is widespread in metazoans and participates in numerous cellular tasks, from gene silencing to chromatin remodeling and protection against retrotransposition. The unicellular eukaryote Trypanosoma cruzi is missing the canonical RNAi pathway and is unable to induce RNAi-related processes. To further understand alternative RNA pathways operating in this organism, we have performed deep sequencing and genome-wide analyses of a size-fractioned cDNA library (16–61 nt) from the epimastigote life stage. Deep sequencing generated 582,243 short sequences of which 91% could be aligned with the genome sequence. About 95–98% of the aligned data (depending on the haplotype) corresponded to small RNAs derived from tRNAs, rRNAs, snRNAs and snoRNAs. The largest class consisted of tRNA-derived small RNAs which primarily originated from the 3′ end of tRNAs, followed by small RNAs derived from rRNA. The remaining sequences revealed the presence of 92 novel transcribed loci, of which 79 did not show homology to known RNA classes

Correlações entre protocolos de determinação do limiar anaeróbio e o desempenho aeróbio em nadadores adolescentes Correlations between anaerobic threshold determination protocols and aerobic performance in adolescent swimmers

O objetivo do presente estudo foi correlacionar as velocidades de limiar anaeróbio (LAn) obtidas a partir de concentrações fixas de lactato (VLAn 4 e VLAn 3,5mM), velocidade de lactato mínimo (VLac min) e velocidade crítica (VC) determinada a partir de diferentes distâncias e número de coordenadas: VC1 (50/100/200m), VC2 (100/200/400m), VC3 (50/100/200/400m) e VC4 (200/400m) com o desempenho nos 400m(s) em nadadores adolescentes. Fizeram parte da amostra 15 nadadores (10 meninos e cinco meninas = 14,7 &plusmn; 0,7 anos; 61,9 &plusmn; 8,5kg; 171,1 &plusmn; 8,8cm) de nível nacional, com experiência entre cinco e sete anos na natação competitiva. Para análise das correlações entre os índices e o desempenho nos 400m(s) foi utilizado o coeficiente de correlação de Pearson. O nível de significância adotado foi de p < 0,01. As correlações entre a VLAn 4mM, VLAn 3,5mM, VLac min e o desempenho nos 400m(s) foram de r = -0,63, r = -0,90 e r = -0,91, respectivamente (p < 0,01). As correlações entre a VC1 (50/100/200m), VC2 (100/200/400m), VC3 (50/100/200/400m), VC4 (200/400m) e o desempenho nos 400m(s) foram: r = -0,62, r = -0,97, r = -0,98 e r = -0,94, respectivamente (p < 0,01). Sugere-se que o LAn determinado a partir da concentração fixa de lactato de 3,5mM, bem como a VLac min e a VC obtidas por meio de distâncias maiores parecem ser os mais adequados índices preditores do desempenho aeróbio nos nadadores adolescentes estudados. Além disso, o número de coordenadas parece não influenciar a relação entre a VC e desempenho aeróbio.<br>The purpose of this study was to correlate the anaerobic threshold speeds (ATS) obtained from fixed lactate blood concentration (ATS 4 e ATS 3.5 mM), lactate minimum speed (Lac minS) and the critical speed (CS) determined from different distances and number of coordinates: CS1 (50/100/200 m), CS2 (100/200/400 m), CS3 (50/100/200/400 m) and CS4 (200/400 m) with the performance in 400 meters (s) in adolescent swimmers. The sample was constituted by 15 swimmers (10 boys and five girls = 14.7 &plusmn; 0.7 years; 61.9 &plusmn; 8.5 kg; 171.1 &plusmn; 8.8 cm) of national level, with five to seven years of experience in competitive swimming. The correlation between the indexes and the performance in 400 m (s) was made using Pearson correlation coefficients. Significance was set at p < 0.01. The correlations between ATS 4 mM, ATS 3.5 mM, Lac minS and the performance in 400 m (s) were: r = -0.63, r = -0.90 e r = 0.91, respectively (p < 0.01). The correlations between CS1 (50/100/200 m), CS2 (100/200/400 m), CS3 (50/100/200/400 m), CS4 (200/400 m) and the performance in 400 m (s) were: r = -0.62, r = -0.97, r = -0.98 e r = -0.94, respectively (p < 0.01). We suggest that ATS obtained from fixed lactate blood concentration of 3.5 mM, as well as Lac minS and the CS obtained through larger distances seem to be the most fitted indexes of prediction of the aerobic performance in the studied adolescent swimmers. Additionally, the number of coordinates seems no influencing the relation between CS and aerobic performance