Vitamin D Receptor Controls Cell Stemness in Acute Myeloid Leukemia and in Normal Bone Marrow.

Vitamin D (VD) is a known differentiating agent, but the role of VD receptor (VDR) is still incompletely described in acute myeloid leukemia (AML), whose treatment is based mostly on antimitotic chemotherapy. Here, we present an unexpected role of VDR in normal hematopoiesis and in leukemogenesis. Limited VDR expression is associated with impaired myeloid progenitor differentiation and is a new prognostic factor in AML. In mice, the lack of Vdr results in increased numbers of hematopoietic and leukemia stem cells and quiescent hematopoietic stem cells. In addition, malignant transformation of Vdr-/- cells results in myeloid differentiation block and increases self-renewal. Vdr promoter is methylated in AML as in CD34+ cells, and demethylating agents induce VDR expression. Association of VDR agonists with hypomethylating agents promotes leukemia stem cell exhaustion and decreases tumor burden in AML mouse models. Thus, Vdr functions as a regulator of stem cell homeostasis and leukemic propagation

Nouveaux marqueurs pronostiques dans les leucémies aiguës myeloïdes : étude par cytométrie en flux des voies de signalisation intracellulaires

REIMS-BU Santé (514542104) / SudocSudocFranceF

ANALYSE D'UNE ZONE RECURRENTE DE DELETION EN 8Q12 DANS LES LEUCEMIES AIGUES LYMPHOBLASTIQUES DE L'ENFANT (DES BIOL. MED.)

CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Anomalies de la signalisation dans les leucémies aiguës myéloïdes

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Etude des marqueurs de la famille du "signaling lymphocyte activating molecule" chez des patients atteints de leucémies aigues myéloïdes

PARIS-BIUP (751062107) / SudocSudocFranceF

Truffe : influence de la taille sur le développement des racines et des mycorhizes de chêne pubescent

National audienc

Automated Detection of Dysplasia: Data Mining from Our Hematology Analyzers

International audienceMyelodysplastic syndromes (MDSs) are clonal hematopoietic diseases of the elderly, charac-terized by chronic cytopenia, ineffective and dysplastic hematopoiesis, recurrent genetic abnormalities and increased risk of progression to acute myeloid leukemia. Diagnosis on a complete blood count (CBC) can be challenging due to numerous other non-neoplastic causes of cytopenias. New genera-tions of hematology analyzers provide cell population data (CPD) that can be exploited to reliably detect MDSs from a routine CBC. In this review, we first describe the different technologies used to obtain CPD. We then give an overview of the currently available data regarding the performance of CPD for each lineage in the diagnostic workup of MDSs. Adequate exploitation of CPD can yield very strong diagnostic performances allowing for faster diagnosis and reduction of time-consuming slide reviews in the hematology laboratory

Generation of a reproducible and stable atmosphere charged of VOC mixture suitable for exposure at air–liquid interface

International audienc

A model of human nasal epithelial cells adapted for direct and repeated exposure to airborne pollutants

International audienceAirway epithelium lining the nasal cavity plays a pivotal role in respiratory tract defense and protection mechanisms. Air pollution induces alterations linked to airway diseases such as asthma. Only very few in vitro studies to date have succeeded in reproducing physiological conditions relevant to cellular type and chronic atmospheric pollution exposure. We therefore, set up an in vitro model of human Airway Epithelial Cells of Nasal origin (hAECN) close to real human cell functionality, specifically adapted to study the biological effects of exposure to indoor gaseous pollution at the environmental level. hAECN were exposed under air-liquid interface, one, two, or three-times at 24 h intervals for 1 h, to air or formaldehyde (200 mu g/m(3)), an indoor air gaseous pollutant. All experiments were ended at day 4, when both cellular viability and cytokine production were assessed. Optimal adherence and confluence of cells were obtained 96 h after cell seeding onto collagen IV-precoated insert. Direct and repeated exposure to formaldehyde did not produce any cellular damage or IL-6 production change, although weak lower IL-8 production was observed only after the third exposure. Our model is significantly better than previous ones due to cell type and the repeated exposure protocol

Skin effect of conductive polymer composites observed by high-resolution solid-state NMR

International audienc