Effects of dynamic cell culture conditions on 3D spatial reogarnization of MSCS/HUVECS spheroids in the context of bone tissue

We aim to observe the development and reorganization of MSCs and HUVECs in Pullulan/Dextran hydrogels supplied with nHAp under dynamic culture conditions in a perfusion bioreactor.Hydrogel poreux sous perfusion : modélisation et optimisation d'un modèle in vitro de reconstruction de défaut osseu

Co-culture of HUVECs and hMSCs in a Perfusion Bioreactor Ensures the Development of Viable Spheroids

International audienc

Effects of dynamic cell culture conditions on 3D spatial reogarnization of MSCS/HUVECS spheroids in the context of bone tissue

International audienceWe aim to observe the development and reorganization of MSCs and HUVECs in Pullulan/Dextran hydrogels supplied with nHAp under dynamic culture conditions in a perfusion bioreactor

Effects of dynamic cell culture conditions on 3D spatial reogarnization of MSCS/HUVECS spheroids in the context of bone tissue

International audienceWe aim to observe the development and reorganization of MSCs and HUVECs in Pullulan/Dextran hydrogels supplied with nHAp under dynamic culture conditions in a perfusion bioreactor

Co-culture of HUVECs and hMSCs in a Perfusion Bioreactor Ensures the Development of Viable Spheroids

International audienc

Perfused porous hydrogel scaffold: modeling hydrodynamics and species transport

International audiencePresent approach well suited to predict both (i) fluid flow in the macroporosity of the scaffold stack, (ii) the oxygen state experienced by the cells within the scaffolds Next: Co-culture of human umbilical vein endothelial cells and human mesenchymal stem cells Optical coherence tomography (OCT) to get a better spatial resolution and capture the microporosity of the porous hydrogel scaffolds Context LBM scheme and boundary conditions Custom-made perfusion bioreactor Hydrodynamics and oxygen transport

How hydrodynamics and species transport influence the 3D microenvironment of cells seeded in hydrogel scaffolds

International audienc

Perfused porous hydrogel scaffold: modeling hydrodynamics and species transport

International audiencePresent approach well suited to predict both (i) fluid flow in the macroporosity of the scaffold stack, (ii) the oxygen state experienced by the cells within the scaffolds Next: Co-culture of human umbilical vein endothelial cells and human mesenchymal stem cells Optical coherence tomography (OCT) to get a better spatial resolution and capture the microporosity of the porous hydrogel scaffolds Context LBM scheme and boundary conditions Custom-made perfusion bioreactor Hydrodynamics and oxygen transport