Antioxidant activity of chloroform extract of Inula Racemosa from Kashmir Himalayas

Antioxidant activity of chloroform extract of root parts of Inula racemosa was evaluated by measuring the scavenging activity of the extract on stable 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH) exhibiting an interesting antioxidant profile. The reducing power was determined using a modification of Zou method. Metal chelating activity of the extract was determined at concentrations of 20, 30, 50 and 100 µg/mL, taking citric acid as standard. The extract displayed significant activity

Optical and electrical characterization of Ni-doped orthoferrites thin films prepared by sol-gel process

International audienceThis paper presents a low-temperature route for producing RFe0.6Ni0.4O3 (where R = Pr, Nd and Sm) thin films by an aqueous inorganic sol-gel process. The films produced were characterized by X-ray diffraction (XRD) for structural, four probes for electrical and UV-vis spectroscopy for optical properties. As-deposited films were amorphous and after annealing them at 650 °C, crystallinity appears and shows an orthorhombic structure. From UV-vis spectroscopy, variation in optical band gap and transmission is seen with change of rare-earth ions. From electrical resistivity measurement, semiconducting behavior is observed. The difference in activation energy is observed. This variation could be due to the orthorhombic distortion caused by size of rare-earth ion and which may impact the Fe-O-Fe or Fe-O-Ni or Ni-O-Ni bond angle, and hence affects the single particle band width in the present system

Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia.

Phytochemical analysis of the dichloromethane:methanol (1:1) extract of root parts of Prangos pabularia led to the isolation of twelve cytotoxic constituents, viz., 6-hydroxycoumarin (1), 7-hydroxycoumarin (2), heraclenol-glycoside (3), xanthotoxol (4), heraclenol (5), oxypeucedanin hydrate (6), 8-((3,3-dimethyloxiran-2-yl)methyl)-7-methoxy-2H-chromen-2-one (7), oxypeucedanin hydrate monoacetate (8), xanthotoxin (9), 4-((2-hydroxy-3-methylbut-3-en-1-yl)oxy)-7H-furo[3,2-g]chromen-7-one (10), imperatorin (11) and osthol (12). The isolates were identified using spectral techniques in the light of literature. 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity screening of the isolated constituents was carried out against six human cancer cell lines including lung (A549 and NCI-H322), epidermoid carcinoma (A431), melanoma (A375), prostate (PC-3) and Colon (HCT-116) cell lines. Osthol (12) exhibited the highest cytotoxicity with IC50 values of 3.2, 6.2, 10.9, 14.5, 24.8, and 30.2 µM against epidermoid carcinoma (A431), melanoma (A375), lung (NCI-H322), lung (A549), prostate (PC-3) and colon (HCT-116) cell lines respectively. Epidermoid carcinoma cell line A431 was sensitive to most of the compounds followed by lung (A549) cancer cell line. Finally a simple and reliable HPLC method was developed (RP-HPLC-DAD) and validated for the simultaneous quantification of these cytotoxic constituents in Prangos pabularia. The extract was analyzed using a reversed-phase Agilent ZORBAX eclipse plus column C18 (4.6×250 mm, 5 µm) at 250 nm wavelength using a gradient water-methanol solvent system at a flow rate of 0.8 ml/min. The RP-HPLC method is validated in terms of recovery, linearity, accuracy and precision (intra and inter-day validation). This method, because of shorter analysis time, makes it valuable for the commercial quality control of Prangos pabularia extracts and its future pharmaceutical preparations

Synthesis and Fabrication of Collagen-Coated Ostholamide Electrospun Nanofiber Scaffold for Wound Healing

A novel scaffold for effective wound healing treatment was developed utilizing natural product bearing collagen-based biocompatible electrospun nanofibers. Initially, ostholamide (OSA) was synthesized from osthole (a natural coumarin), characterized by ¹H, ¹³C, DEPT-135 NMR, ESI-MS, and FT-IR spectroscopy analysis. OSA was incorporated into polyhydroxybutyrate (PHB) and gelatin (GEL), which serve as templates for electrospun nanofibers. The coating of OSA-PHB-GEL nanofibers with collagen resulted in PHB-GEL-OSA-COL nanofibrous scaffold which mimics extracellular matrix and serves as an effective biomaterial for tissue engineering applications, especially for wound healing. PHB-GEL-OSA-COL, along with PHB-GEL-OSA and collagen film (COLF), was characterized <i>in vitro</i> and <i>in vivo</i> to determine its efficacy. The developed PHB-GEL-OSA-COL nanofibers posed an impressive mechanical stability, an essential requirement for wound healing. The presence of OSA had contributed to antimicrobial efficacy. These scaffolds exhibited efficient antibacterial activity against common wound pathogens, <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>). The zones of inhibition were observed to be 14 ± 22 and 10 ± 2 mm, respectively. It was observed that nanofibrous scaffold had the ability to release OSA in a controlled manner, and hence, OSA would be present at the site of application and exhibit bioactivity in a sustained manner. PHB-GEL-OSA-COL nanofiber was determined to be stable against enzymatic degradation, which is the most important parameter for promoting proliferation of cells contributing to repair and remodeling of tissues during wound healing applications. As hypothesized, PHB-GEL-OSA-COL was observed to imbibe excellent cytocompatibility, which was determined using NIH 3T3 fibroblast cell proliferation studies. PHB-GEL-OSA-COL exhibited excellent wound healing efficacy which was confirmed using full thickness excision wound model in Wistar rats. The rats treated with PHB-GEL-OSA-COL nanofibrous scaffold displayed enhanced healing when compared to untreated control. Both <i>in vitro</i> and <i>in vivo</i> analysis of PHB-GEL-OSA-COL presents a strong case of therapeutic biomaterial suiting wound repair and regeneration

Intra and inter-day precisions area of HPLC method of marker compounds <b>1–12</b>.

<p>Intra and inter-day precisions area of HPLC method of marker compounds <b>1–12</b>.</p

Limits of detection (LOD) and quantification (LOQ) of marker compounds <b>1–12</b>.

<p>a) The calibration curves were constructed by plotting the peak areas versus the concentration of each analyte.</p><p>b) LOD refers to the limit of detection.</p><p>c) LOQ refers to the limit of quantification.</p><p>Limits of detection (LOD) and quantification (LOQ) of marker compounds <b>1–12</b>.</p

Chemical structure of marker compounds isolated from plant Prangos pabularia.

<p>(<b>1</b>), 6-hydroxo-coumarin (<b>2</b>), Umbelliferone (<b>3</b>), Heraclenol glycoside (<b>4</b>), Xanthotoxol (<b>5</b>), Heraclenol (<b>6</b>), Oxypeucedanin hydrate (<b>7</b>), 8-((3,3-dimethyloxiran-2-yl)methyl)-7-methoxy-2H-chromen-2-one (Merangin) (<b>8</b>), Oxypeucedanin hydrate monoacetate (<b>9</b>), Xanthotoxin (<b>10</b>), 4-((2-hydroxy-3-methylbut-3-en-1-yl)oxy)-7H-furo[3,2-g]chromen-7-one (<b>11</b>), Imperatorin (<b>12</b>), Osthol.</p

Recovery percentage of marker compounds <b>1–12</b>.

<p>Recovery percentage of marker compounds <b>1–12</b>.</p