A post COVID lockdown pilot study on the effect of physical activity intensity on cognitive functions of children aged 6-8 years old

Physical activity practice is very important for individual\u2019s wealth status. Moreover, it is possible to consider physical activity one of the teachers\u2019 tool to improve the academic achievement and the cognitive functions in children. However, according to some evidences it seems that the physical activity at high intensity increase the cognitive functions compared to the low intensity physical activity. In this project, we investigate how the intensity of physical activity affects a series of cognitive functions such as Attention and Working Memory. Eleven participants of a summer camp participated at the study. Three experimental weeks with different physical intensities were involved (Standard Intensity, High intensity and Low Intensity). Participants performed the cognitive tasks at the beginning and at the end of each experimental week. Our results indicated an effect of the physical activity intensity in some kind of attention. Working Memory instead, was not affected. Thus, our results suggested that intensity could be an important factor to increase some cognitive functions, but not all of them

A post COVID lockdown pilot study on the effect of physical activity intensity on cognitive functions of children aged 6-8 years old

Physical activity practice is very important for individual’s wealth status. Moreover, it is possible to consider physical activity one of the teachers’ tool to improve the academic achievement and the cognitive functions in children. However, according to some evidences it seems that the physical activity at high intensity increase the cognitive functions compared to the low intensity physical activity. In this project, we investigate how the intensity of physical activity affects a series of cognitive functions such as Attention and Working Memory. Eleven participants of a summer camp participated at the study. Three experimental weeks with different physical intensities were involved (Standard Intensity, High intensity and Low Intensity). Participants performed the cognitive tasks at the beginning and at the end of each experimental week. Our results indicated an effect of the physical activity intensity in some kind of attention. Working Memory instead, was not affected. Thus, our results suggested that intensity could be an important factor to increase some cognitive functions, but not all of them

Impact of a mediterranean dietary pattern and its components on cardiovascular risk factors, glucose control, and body weight in people with type 2 diabetes: A real-life study

This study evaluates the relation of a Mediterranean dietary pattern and its individual components with the cardiovascular risk factors profile, plasma glucose and body mass index (BMI) in people with type 2 diabetes. We studied 2568 participants at 57 diabetes clinics. Diet was assessed with the EPIC (European Prospective Investigation into Cancer and Nutrition) questionnaire, adherence to the Mediterranean diet was evaluated with the relative Mediterranean diet score (rMED). A high compared to a low score was associated with a better quality of diet and a greater adherence to the nutritional recommendations for diabetes. However, even in the group achieving a high score, only a small proportion of participants met the recommendations for fiber and saturated fat (respectively 17% and 30%). Nonetheless, a high score was associated with lower values of plasma lipids, blood pressure, glycated hemoglobin, and BMI. The relationship of the single food items components of the rMED score with the achievement of treatment targets for plasma lipids, blood pressure, glucose, and BMI were also explored. The study findings support the Mediterranean dietary model as a suitable model for type 2 diabetes and the concept that the beneficial health effects of the Mediterranean diet lie primarily in its synergy among various nutrients and foods rather than on any individual component

Micro-RNA and Proteomic Profiles of Plasma-Derived Exosomes from Irradiated Mice Reveal Molecular Changes Preventing Apoptosis in Neonatal Cerebellum

Cell communication via exosomes is capable of influencing cell fate in stress situations such as exposure to ionizing radiation. In vitro and in vivo studies have shown that exosomes might play a role in out-of-target radiation effects by carrying molecular signaling mediators of radiation damage, as well as opposite protective functions resulting in resistance to radiotherapy. However, a global understanding of exosomes and their radiation-induced regulation, especially within the context of an intact mammalian organism, has been lacking. In this in vivo study, we demonstrate that, compared to sham-irradiated (SI) mice, a distinct pattern of proteins and miRNAs is found packaged into circulating plasma exosomes after whole-body and partial-body irradiation (WBI and PBI) with 2 Gy X-rays. A high number of deregulated proteins (59% of WBI and 67% of PBI) was found in the exosomes of irradiated mice. In total, 57 and 13 miRNAs were deregulated in WBI and PBI groups, respectively, suggesting that the miRNA cargo is influenced by the tissue volume exposed to radiation. In addition, five miRNAs (miR-99b-3p, miR-200a-3p, miR-200a, miR-182-5p, miR-182) were commonly overexpressed in the exosomes from the WBI and PBI groups. In this study, particular emphasis was also given to the determination of the in vivo effect of exosome transfer by intracranial injection in the highly radiosensitive neonatal cerebellum at postnatal day 3. In accordance with a major overall anti-apoptotic function of the commonly deregulated miRNAs, here, we report that exosomes from the plasma of irradiated mice, especially in the case of WBI, prevent radiation-induced apoptosis, thus holding promise for exosome-based future therapeutic applications against radiation injury

Translational Research in the Era of Precision Medicine: Where We Are and Where We Will Go

The advent of Precision Medicine has globally revolutionized the approach of translational research suggesting a patient-centric vision with therapeutic choices driven by the identification of specific predictive biomarkers of response to avoid ineffective therapies and reduce adverse effects. The spread of "multi-omics" analysis and the use of sensors, together with the ability to acquire clinical, behavioral, and environmental information on a large scale, will allow the digitization of the state of health or disease of each person, and the creation of a global health management system capable of generating real-time knowledge and new opportunities for prevention and therapy in the individual person (high-definition medicine). Real world data-based translational applications represent a promising alternative to the traditional evidence-based medicine (EBM) approaches that are based on the use of randomized clinical trials to test the selected hypothesis. Multi-modality data integration is necessary for example in precision oncology where an Avatar interface allows several simulations in order to define the best therapeutic scheme for each cancer patient

The interplay between radioresistant Caco-2 cells and the immune system increases epithelial layer permeability and alters signaling protein spectrum

Colorectal cancer is one of the most frequent type of cancer, with a higher incidence in the developed countries. Colorectal cancer is usually managed with both surgeries, chemotherapy and radiotherapy. Radiotherapy has the well-known advantage of targeting the tumor, minimizing normal tissue exposure. Nevertheless, during radiation treatment, exposure of healthy tissues is of great concern, in particular because of the effects on the intestinal barrier functions and on cells belonging to the immune system. The functional role of intestinal barrier in avoiding paracellular trafficking and controlling bacterial spread from gut it is well known and it is due to the presence of tight junction complexes. However, intestinal barrier is fundamental in participating to the interplay with immune system, especially considering the gut-associated lymphoid tissue. Until few years ago, radiotherapy was considered to bear only a depressive action on the immune system. However, it is now recognized that the release of pro-inflammatory signals and phenotypic changes in tumoral cells due to ionizing radiation could trigger the immune system against the tumor. In this work, we address how intestinal barrier functions are perturbed by X-ray doses in the range 0-10 Gy, focusing on the interplay between tumoral cells and the immune system. To this aim, we adopted a coculture model in which Caco-2 cells can be grown in presence/absence of peripheral blood mononuclear cells (PBMC). We focused our attention on changes in the proliferation, trans-epithelial electrical resistance (TEER), cytokine release, and proteins of the junctional complexes. Our results indicate a high radioresistance of Caco-2 in the investigated dose range, and an increased permeability of the tumoral cell layer due to the presence of PBMC. This is found to be correlated with activation of PBMC, inhibiting the apoptotic pathway, with the enhancement of cytokine release and with variation of tight junction scaffold protein expression levels, assumed to be related to IFN-gamma-and TNF-alpha-mediated signaling

A post COVID Lockdown pilot study on the effect of PA intensity on cognitive function in male children aged 6-8 years old.

In a sedentary world (WHO, 2020), physical activity seems an important tool that helps the development of cognitive functions (Tomporoski et al. 2011). However, the majority of the studies investigated the effect through cross sectional studies while only few ones are interventional. In this interventional experiment we focused our attention on how the physical activity modulated a pletory of cognitive functions. Specifically, during three weeks of a summer camp, 13 children performed physical activities at various intensities. In the first week, participants performed activities without any particular suggestions, in the second and third week children performed physical activity at high and low intensity, respectively. Children performed at the first (Baseline) and the last day of the summer camp a series of cognitive function tests in order to understand whether the level of physical activity employed in the Summer Camp week increased the cognitive functions tested. In particular they performed an Attention task (Posner Cueing task, Chun, 2000) and both Working Memory Forward (WMF) and Backwards (WMB) tasks. Results highlighted an increment of the cognitive functions tested: reaction times (RT) of the Attention task were significantly faster in high intensity week compared to the baseline while in the other two experimental weeks this improvement was not occured; similar results in both Working Memory Forward and Backward were found

Investigation of the mechanisms underpinning IL-6 cytokine release in bystander phenomena: the roles of radiation dose, radiation quality and specific ROS/RNS scavengers.

Purpose: To investigate the mechanisms regulating the pathways of the bystander transmission in vitro, focusing on the radiation-perturbed signalling (via Interleukine 6, IL-6) of the irradiated cells after exposure to low doses of different radiation types. Materials and methods: An integrated 'systems radiation biology' approach was adopted. Experimentally the level of the secreted cytokine from human fibroblasts was detected with ELISA (Enzyme-Linked ImmunoSorbent Assay) method and subsequently the data were analyzed and coupled with a phenomenological model based on differential equations to evaluate the single-cell release mechanisms. Results: The data confirmed the important effect of radiation on the IL-6 pathway, clearly showing a crucial role of the ROS (Reactive Oxygen Species) in transducing the effect of initial radiation exposure and the subsequent long-term release of IL-6. Furthermore, a systematic investigation of radiation dose/radiation quality dependence seems to indicate an increasing efficiency of high LET (Linear Energy Transfer) irradiation in the release of the cytokine. Basic hypotheses were tested, on the correlation between direct radiobiological damage and signal release and on the radiation target for this endpoint (secretion of IL-6) Conclusions: The results demonstrate the role of reactive oxygen and nitrogen species in the signaling pathways of IL-6. Furthermore the systems radiation biology approach here adopted, allowed us to test and verify hypotheses on the behavior of the single cell in the release of cytokine, after the exposure to different doses and different qualities of ionizing radiation