Experimental Evolution of a Plant Pathogen into a Legume Symbiont

Following acquisition of a rhizobial symbiotic plasmid, adaptive mutations in the virulence pathway allowed pathogenic Ralstonia solanacearum to evolve into a legume symbiont under plant selection

Learning and memory of cue-reward association meaning by modifications of synaptic efficacy in dentate gyrus and piriform cortex

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Root nodulation of Sesbania rostrata.

The tropical legume Sesbania rostrata can be nodulated by Azorhizobium caulinodans on both its stem and its root system. Here we investigate in detail the process of root nodulation and show that nodules develop exclusively at the base of secondary roots. Intercellular infection leads to the formation of infection pockets, which then give rise to infection threads. Concomitantly with infection, cortical cells of the secondary roots dedifferentiate, forming a meristem which has an "open-basket" configuration and which surrounds the initial infection site. Bacteria are released from the tips of infection threads into plant cells via "infection droplets," each containing several bacteria. Initially, nodule differentiation is comparable to that of indeterminate nodules, with the youngest meristematic cells being located at the periphery and the nitrogen-fixing cells being located at the nodule center. Because of the peculiar form of the meristem, Sesbania root nodules develop uniformly around a central axis. Nitrogen fixation is detected as early as 3 days following inoculation, while the nodule meristem is still active. Two weeks after inoculation, meristematic activity ceases, and nodules then show the typical histology of determinate nodules. Thus, root nodule organogenesis in S. rostrata appears to be intermediate between indeterminate and determinate types

Measurement of delayed neutrons in a thermal nuclear reactor by means of a long run pile noise experiment in sub-critical state

The transfer function of a zero-power thermal reactor was successfully measured thanks to neutron noise techniques from 1 mHz to 160 Hz. During a month-long experimental campaign, the fluctuations of the neutron population in critical and subcritical configurations of the core were acquired using excore fission chambers and analysed through Cross-Power Spectral Density (CPSD) methodology. Firstly, the reactor’s kinetic parameters, i.e. prompt decay constant, effective delayed neutron fraction and generation time, were obtained at critical state. It required calibrating the reactor’s power, which was done by metal foil activation and measurement of the 235U fission rate. Secondly, these parameters were used to estimate the groups’ abundances of delayed neutrons from the CPSD measured in a sub-critical state. Fitting data with a point kinetic model was done with Bayesian inference - CONRAD and Stan programs were used. A very good agreement was found between experimental abundances and the ones computed with TRIPOLI-4 Monte-Carlo transport code and JEFF3.1.1 nuclear data library. Uncertainties on prior abundances between 6 % to 101 %, held mainly by nuclear data, were lowered down to 4 % to 54 %, depending on the delayed group

Increase in polysialyltransferase gene expression following LTP in adult rat dentate gyrus

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