Following acquisition of a rhizobial symbiotic plasmid, adaptive mutations in the virulence pathway allowed pathogenic Ralstonia solanacearum to evolve into a legume symbiont under plant selection

A Buckling

A. M Hirsch

B Brito

Béatrice Chane-Woon-Ming

C Amadou

C Faucher

C Masson-Boivin

C. A Boucher

C. J Marx

Carine Gris

Catherine Masson-Boivin

Claudine Médigue

D Aldon

D DeShazer

D MacLean

D Meyer

D. J Gage

Delphine Capela

E Martinez

E Martinez-Romero

E. T Kiers

F Van Gijsegem

G Fahraeus

G Truchet

G. E. D Oldroyd

Graham C. Walker

J Batut

J Plazinski

J Quandt

J Sullivan

J Vasse

J. H Miller

J. L Sachs

J. T Sullivan

Jacques Batut

L Deslandes

L Moulin

Luz B. Gilbert

M Arlat

M Poueymiro

M Rogel

M Salanoubat

M Valls

M. F Hynes

M. J Soto

Marta Marchetti

Michelle Glew

O Gotoh

P Vandamme

Philipp Heeb

S Cunnac

S Genin

S. L Turner

S. R Long

Stéphane Cruveiller

T Ferenci

T. F Smith

Ton Timmers

Véréna Poinsot

W. J Deakin

W. M Chen

Y. G Liu

Z. M Ning

English

PubMed

Crossref

Experimental Evolution of a Plant Pathogen into a Legume Symbiont

International audienceAuthor Summary / Most leguminous plants can form a symbiosis with members of a group of soil bacteria known as rhizobia. On the roots of their hosts, some rhizobia elicit the formation of specialized organs, called nodules, that they colonize intracellularly and within which they fix nitrogen to the benefit of the plant. Rhizobia do not form a homogenous taxon but are phylogenetically dispersed bacteria. How such diversity has emerged is a fascinating, but only partly documented, question. Although horizontal transfer of symbiotic plasmids or groups of genes has played a major role in the spreading of symbiosis, such gene transfer alone is usually unproductive because genetic or ecological barriers restrict evolution of symbiosis. Here, we experimentally evolved the usually phytopathogenic bacterium Ralstonia solanacearum, which was carrying a rhizobial symbiotic plasmid into legume-nodulating and - infecting symbionts. From resequencing the bacterial genomes, we showed that inactivation of a single regulatory gene allowed the transition from pathogenesis to legume symbiosis. Our findings indicate that following the initial transfer of symbiotic genes, subsequent genome adaptation under selection in the plant has been crucial for the evolution and diversification of rhizobia

Marchetti, Marta

Capela, Delphine

Glew, Michelle

Cruveiller, Stéphane

Chane-Woon-Ming, Béatrice

Gris, Carine

Timmers, Ton

Poinsot, Verena

Gilbert, Beatriz-Luz

Heeb, Philipp

Medigue, Claudine

Batut, Jacques

Masson-Boivin, Catherine

HAL-INSA Toulouse

HAL-CEA

HAL Evry

HAL: Hyper Article en Ligne

HAL-IRD

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Trigalet A

Experimental Evolution of a Plant Pathogen into a Legume Symbiont

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