The adsorption of proteins onto ultrafiltration membranes

The mass of five proteins (Bovine serum albumin (BSA), casein, lysozyme, ovalbumin and pepsin) adsorbed to five different membrane materials (of various hydrophobicities) was quantified using a static system and analysed to establish any trends. Comparing the results from the five membranes it seems that there were no obvious trends between the protein masses adsorbed indicating that it may not be just one aspect of protein structure that is important in the adsorption process. Many investigations have indicated that the protein may undergo a conformational change during the adsorption process. Disulphide bridges contribute readily to the stability of the protein molecule and it was hypothesised that if such a structural change occurred, it would result in the breakage of these covalent bonds. To this end, the free thiol group content of the proteins was quantified before and after adsorption. [Continues.

Grating coupled photonic crystal demultiplexer with integrated detectors on InP-membrane

We report on the successful integration of grating fiber couplers, compact photonic crystal demultiplexers and efficient p-i-n photodetectors on a single bonded InP-membrane chip. Polarization independent operation is obtained by implementing polarization diversity

Gamma-Ray Spectra & Variability of the Crab Nebula Emission Observed by BATSE

We report ~ 600 days of BATSE earth-occultation observations of the total gamma-ray (30 keV to 1.7 MeV) emission from the Crab nebula, between 1991 May 24 (TJD 8400) and 1994 October 2 (TJD 9627). Lightcurves from 35-100, 100-200, 200-300, 300-400, 400-700, and 700-1000 keV, show that positive fluxes were detected by BATSE in each of these six energy bands at significances of approximately 31, 20, 9.2, 4.5, 2.6, and 1.3 sigma respectively per day. We also observed significant flux and spectral variations in the 35-300 keV energy region, with time scales of days to weeks. The spectra below 300 keV, averaged over typical CGRO viewing periods of 6-13 days, can be well described by a broken power law with average indices of ~ 2.1 and ~ 2.4 varying around a spectral break at ~ 100 keV. Above 300 keV, the long-term averaged spectra, averaged over three 400 d periods (TJD 8400-8800, 8800-9200, and 9200-9628, respectively) are well represented by the same power law with index of ~ 2.34 up to ~ 670 keV, plus a hard spectral component extending from ~ 670 keV to ~ 1.7 MeV, with a spectral index of ~ 1.75. The latter component could be related to a complex structure observed by COMPTEL in the 0.7-3 MeV range. Above 3 MeV, the extrapolation of the power-law continuum determined by the low-energy BATSE spectrum is consistent with fluxes measured by COMPTEL in the 3-25 MeV range, and by EGRET from 30-50 MeV. We interpret these results as synchrotron emission produced by the interaction of particles ejected from the pulsar with the field in different dynamical regions of the nebula system, as observed recently by HST, XMM-Newton, and Chandra.Comment: To be published in the November 20, 2003, Vol 598 issue of the Astrophysical Journa

Critical values for Lawshe's content validity ratio: revisiting the original methods of calculation

YesThe content validity ratio originally proposed by Lawshe is widely used to quantify content validity and yet methods used to calculate the original critical values were never reported. Methods for original calculation of critical values are suggested along with tables of exact binomial probabilities

Attention and fluctuating attention in patients with dementia with lewy bodies and alzheimer disease

Background: Attentional deficits are described in the consensus clinical criteria for the operationalized diagnosis of dementia with Lewy bodies (DLB) as characteristic of the condition. In addition, preliminary studies have indicated that both attentional impairments and fluctuation of attentional impairments are more marked in patients with DLB than in patients with Alzheimer disease (AD), although neuropsychological function has not previously been examined in a large prospective cohort with confirmed diagnostic accuracy against postmortem diagnosis. Methods: A detailed evaluation of attention and fluctuating attention was undertaken in 155 patients with dementia (85 with DLB and 80 with AD) from a representative hospital dementia case register and 35 elderly controls using the Cognitive Drug Research Computerized Assessment System for Dementia Patients computerized neuropsychological battery. Operationalized clinical diagnosis was made using the consensus criteria for DLB and the National Institute of Neurological and Communicative Disorders and Stroke-Alzheimer's Disease and Related Disorders Association criteria for AD. High levels of sensitivity and specificity have been achieved for the first 50 cases undergoing postmortem examination. Results: The groups were well matched for severity of cognitive impairments, bur the AD patients were older (mean age, 80 vs 78 years) and more likely to be female (55 vs 40). Patients with DLB were significantly more impaired than patients with AD on all measures of attention and fluctuating attention (for all comparisons, t � 2.5, P<.001), and patients from both dementia groups were significantly more impaired than elderly controls for all comparisons other than cognitive reaction time, which was significantly more impaired in DLB patients than controls but was comparable in controls and AD patients. There were, however, significant associations between the severity of cognitive impairment and the severity of both attentional deficits and fluctuations in attention. Conclusions: This large prospective study confirms that slowing of cognitive processing, attention, and fluctuations of attention are significantly more pronounced in DLB and AD patients, although fluctuating attention is common in patients with moderate-to-severe AD. Deficits of cognitive reaction rime appear to be specific to DLB, except in severe dementia. A detailed evaluation of attentional performance could make an important contribution to differential diagnosis, although the results need to be interpreted within the context of the overall severity of cognitive deficits

Towards a Framework for Open Data Publishers:A Comparison Study between Sweden and Belgium

Public organizations in the role of publishers publish datafor anyone to reuse, which can lead to benets. However, the processdescriptions for this publishing work focus on one or a few issues, whichleaves out important areas and decisions. Little seems to be known aboutvariations between publishers based on one common point of comparison.Therefore, this paper presents a comparison between two publishers:Namur (Belgium) and Linkoping (Sweden). The comparison is based ona process framework, seven in-depth interviews, document studies, anda verication meeting with one respondent. We learned that the OGD manager is an agent of change who need to balance implementation andguidance, the orthodox method of e-mail registration can be used toengage users and monitor impact, the organizational unit for OGD iscross-organizational, and the publisher process framework could be usedas ex-ante strategic guidelines and context-specic recommendations