Sensitivity of epicardial electrical markers to acute ischemia detection

pre-printIntroduction: We hypothesize that electrocardiographic measurements from the intramyocardial space contain more sensitive markers of ischemia than those detectable on the epicardium. The goal of this study was to evaluate different electrical markers for their potential to detect the earliest phases of acute myocardial ischemia. Methods: We conducted acute ischemia studies in open chest animal, by creating finely controlled demand or supply ischemic episodes and recording intramyocardial and epicardial potentials. Results: Under the conditions of mild perfusion deficit, acute ischemia induced changes in the T wave that were larger and could be detected earlier on the epicardial surface than ST-segment changes. Conclusions: Our findings indicate that in the setting of very acute ischemia, epicardial T waves have higher sensitivity to mild degrees of acute ischemia than epicardial ST potentials. These results suggest that changes in the T wave shape may augment shifts in ST segments to improve ECG based localization of ischemia

Molecular diversity in persimmon (Diospyros kaki L.) cultivars growing around Hatay province in Turkey

Genetic relationships among Diospyros kaki L. growing around Hatay province in Turkey were assessed by randomly amplified polymorphic DNA (RAPD) assay. Ten decamer primers were selected from 50 primers. These primers yielded a total of 155 bands and 126 of them were recorded aspolymorphic. Pairwise genetic distances of the samples were used to construct dendograms using Unweighted Pair-Group Method of Arithmetic Average (UPGMA). The study suggests that the morphological differences among cultivars of persimmon might be the result of genetic differencesrather than the ecological or growing conditions. The study forms a basic contribution to the characterization of D. kaki population in Turkey

Molecular characterization of salt stress in grapevine cultivars (Vitis vinifera L.) and rootstocks

Salt stress is an important factor inducing the expression of many genes; e.g. the osmotin gene is one of the very important genes responding to NaCl stress. After exposure to NaCl stress, the osmotin gene expression level was investigated in 6 grape cultivars grown in GAP (Southeast Anatolian region including the provinces Gaziantep, Şanliurfa, Diyarbakır, Mardin, Batman, Siirt and Şırnak) and in 4 grape cultivars and 7 rootstocks recommended to the GAP region. Expression levels were investigated by Northern blot analysis. The osmotin expression level was higher in scion cultivars than in rootstocks. Within grapevine cultivars, the highest osmotin gene expression level was observed in cv. Tahannebi, followed by Hönüsü, another GAP region cultivar. The osmotin gene expression levels of two grape cultivars, Ata sarisi and Alphonse Lavallée recommended to the GAP region, were lower than those of Hönüsü. On the other hand the GAP region cultivars Ağ Besni, Rumi, Kabarcık, Dımıþkı and the recommended cultivars Razakı and Italia were significantly different in their expression levels. Among rootstocks 1616 C displayed the highest expression level, followed by 99R; they were followed by 1613C. In 110R the expression level was slightly lower than in the above mentioned ones and 41B. In SO4 and 5C the level of expression remained at the control level.

GM1 Ganglioside Modifies α-Synuclein Toxicity and is Neuroprotective in a Rat α-Synuclein Model of Parkinson\u27s Disease.

While GM1 may interact with α-synuclein in vitro to inhibit aggregation, the ability of GM1 to protect against α-synuclein toxicity in vivo has not been investigated. We used targeted adeno-associated viral vector (AAV) overexpression of human mutant α-synuclein (A53T) in the rat substantia nigra (SN) to produce degeneration of SN dopamine neurons, loss of striatal dopamine levels, and behavioral impairment. Some animals received daily GM1 ganglioside administration for 6 weeks, beginning 24 hours after AAV-A53T administration or delayed start GM1 administration for 5 weeks beginning 3 weeks after AAV-A53T administration. Both types of GM1 administration protected against loss of SN dopamine neurons and striatal dopamine levels, reduced α-synuclein aggregation, and delayed start administration of GM1 reversed early appearing behavioral deficits. These results extend prior positive results in MPTP models, are consistent with the results of a small clinical study of GM1 in PD patients that showed slowing of symptom progression with chronic use, and argue for the continued refinement and development of GM1 as a potential disease modifying therapy for PD

Selective Jamming of LoRaWAN using Commodity Hardware

Long range, low power networks are rapidly gaining acceptance in the Internet of Things (IoT) due to their ability to economically support long-range sensing and control applications while providing multi-year battery life. LoRa is a key example of this new class of network and is being deployed at large scale in several countries worldwide. As these networks move out of the lab and into the real world, they expose a large cyber-physical attack surface. Securing these networks is therefore both critical and urgent. This paper highlights security issues in LoRa and LoRaWAN that arise due to the choice of a robust but slow modulation type in the protocol. We exploit these issues to develop a suite of practical attacks based around selective jamming. These attacks are conducted and evaluated using commodity hardware. The paper concludes by suggesting a range of countermeasures that can be used to mitigate the attacks.Comment: Mobiquitous 2017, November 7-10, 2017, Melbourne, VIC, Australi

Genetic relationships among some Hesperis L. (Brassicaceae) species from Turkey assessed by RAPD analysis

In this study the phylogenetic relations among infraspecific, specific and supraspecific categories of 6 taxa of the genus Hesperis collected from different parts of Turkey were investigated by RAPD analysis.The results of the RAPD analysis support the idea that H. bicuspidata (Sect. Hesperis), H. schischkinii (Sect. Mediterranea), H. pendula (Sect. Pachycarpos), H. breviscapa, H. kotschyi (Sect. Cvelevia) and H.cappadocica (Sect. Contorta) species need to be placed into different sections according to morphological characters. On the other hand, the phylogenetic order of the sections according to morphological characters and according to molecular data displayed some differences and evolutionary phylogenetic orders of the sections were redesigned. The phylogenetic relations among species were based on the samples H. breviscapa and H. kotschyi which take place in the same section.The accordance of morphological and molecular similarities was noticed for H. breviscapa and H. kotschyi species. Besides this, infraspecific taxonomic situations of H. schischkinii samples havinghairy and glabrous (non-hairy) fruits which show allopatric and sympatric spread were reassessed by RAPD analysis

Analysis of Organic Matter in Sediments and Macrozoobenthos From Coastal Waters of Hurun Bay of Lampung

This study was conducted in July 2015 with the aim to observe the macrozoobenthospopulation and organic matter content in the sediment. The samples were collected from HurunBay in Lampung Province with using survey method. Samples were analyzed in the Laboratoryof Water Quality BBPPL Lampung and Chemistry Laboratory, Faculty of Fisheries and MarineSciences University of Riau. The study showed that the organic content in the sediment between22.88 - 54.91%, while the abundance of macrozoobenthos was between 20.29 - 66.67 ind / m2.Macrozoobenthos identified in Hurun Bay 8 species, 3 classes and 2 phylua. From the phylum ofMollusca there were Gastropod i.e. which had 5 species and from the classes of bivalves thestudy also found two species. From the phylum annelid there was one class, Polychaeta and onespecies, i.e. Nereis sp. The relationship between macrozoobenthos and organic content wasexpressed with the equation of Y = 9,411 + 0,6268X that the relationship of organic matter withmacrozoobenthos abundance was weak

Determination of Physiological, Biochemical and Molecular Effects of Zinc Stress on the Growth of Sunflower Seedlings (Helianthus annuus L.)

Heavy metal contamination is an important environmental problem all over the world. High concentrations of heavy metals cause permanent damage stocells and tissues. In this study, the toxic effects of zincheavy metal in sunflower plant in population and molecular parameters were investigated. The effects of zincheavymetalon sunflower seedlings were determined using the changes in population parameters; rootlength, dry weight, and total solubleprotein content. Total protein content of sunflower plants was determined in a relationship in the opposite direction increasing the amount of metal concentrations. Genotoxic effects of heavymetal of zinc on sunflower plants were revealed by using changes in genomic template stability (GTS). According to analyses, serious changes in genomic template stability were detected and these results were compared with the growth, dry weight and total soluble protein content of the seedlings grown at various zinc concentrations. Also, it was seen that the genomic template stability significantly affected the primary root length, root dry weight and root total soluble protein content. Positive correlations were observed between physiological, biochemical and molecular parameters in sunflower seedlings under zinc stress.In conclusion, a comparison between physiological, biochemical and molecular parameters shows that zinc is a genotoxic agent for sunflower plants