Synthetic dyes decolorization of new Trametes fungus isolates

U ovom radu istražena je sposobnost obezbojenja sintetskih bojila (fuksin, kongo crvenilo i kristal violet) pomoću novih, do sada neistraženih izolata gljiva bijelog truljenja Trametes versicolor (L.) i Trametes gibbosa (Pers.) Fr. Istraživanjima sposobnosti obezbojenja prethodili su kvalitativni testovi za određivanje celulolitičke i lignolitičke aktivnosti koji su bili pozitivni za obje vrste gljiva. Gljive su uzgajane na agarnim pločama s dodatkom bojila u koncentracijama 10, 30, 50, 100 i 150 mg L$^{-1}$, te je kroz 14 dana praćen njihov porast i promjer obezbojenja. Nakon 14 dana, pri sobnoj temperaturi, nastavljeno je praćenje obezbojenje podloga do potpunog obezbojenja cijele agarne površine. Nakon 28 dana uzgoja gljiva T. versicolor TV8 je u potpunosti obezbojila cijelu površinu agarnih ploča s dodatkom kongo crvenila u svim odabranim koncentracijama te agarnih ploča s dodatkom fuksina i kristal violeta u svim koncentracijama, osim 150 mg L$^{-1}$. Gljiva T. gibbosa, potpuno je obezbojila cijelu površinu agarnih ploča s dodatkom kongo crvenila osim u najvećoj koncentraciji od 150 mg L$^{-1}$. U slučaju fuksina potpuno su obezbojene samo agarne ploče s dodatkom bojila u koncentracijama 10 i 30 mg L$^{-1}$, a ploče s dodatkom kristal violeta samo u koncentraciji od 10 mg L$^{-1}$. Nadalje, ispitana je mogućnost obezbojenja kongo crvenila pomoću T. versicolor u uvjetima fermentacije na čvrstim nosačima, pri čemu je kao supstrat i nosač korišten obojeni pivski trop. Tijekom uzgoja u trajanju od 21 dan, došlo je do porasta udjela proteina u uzorku te gubitka na masi supstrata od 17 % u odnosu na abiotičku kontrolu. Tijekom fermentacije na čvrstim nosačima došlo je do djelomičnog obezbojenja pivskog tropa što je potvrđeno vizualno i određivanjem promjene parametara boje.This study investigated the ability of newly isolated white rot fungi Trametes versicolor (L.) and Trametes gibbosa (Pers.) Fr. to decolorize synthetic dyes (fuchsine, congo red and crystal violet). Prior to decolorization tests, qualitative test for determination of cellulolytic and lignolytic activity were performed and were positive for both fungi. The fungi were cultivated on agar plates containing dyes at concentrations of 10, 30, 50, 100 and 150 mg L$^{-1}$ for 14 days. The radial growth and the zone of colour change on the agar plates were measured regularly every two days. The agar plates were then stored at room temperature and the change of colour was monitored visually for another 14 days. After 28 days, T. versicolor TV8 completely decolorize all agar plates containing congo red at all the applied concentrations. Apart from agar plates with the addition of 150 mg L$^{-1}$ fuchsine and crystal violet, all other agar plates with the adition of fuchsine and crystal violet were successfully decolorized. T. gibbosacompletely decolorized all agar plates containing congo red except at the highest concentration of 150 mg L$^{-1}$. Complete decolorization of agar plates containing fuchsine was only noted for concentrations of 10 and 30 mg L$^{-1}$, while in case of crystal violet the complete decolorization only occurred on agar plates with added concentration of crystal violet of 10 mg L$^{-1}$. Furthermore, T. versicolor was cultivated under solid-state conditions using congo red loaded brewers' spent grain as a carrier and substrate. T. versicolor grew readily under solid-state conditions, which was confirmed by the increase of protein content and substrate mass loss of 17% compared to abiotic control. Only partial decolorisation of congo red was observed, which was confirmed visually and by measuring the changes of color parameters

Decolourisation of synthetic dyes by fungal pellets

Obojene otpadne vode koje se ispuštaju u prirodne prijemnike, a sadrže velike koncentracije sintetskih bojila, važan su izvor onečišćenja površinskih i podzemnih voda. Prisutnost bojila u industrijskim otpadnim vodama predstavlja problem zbog izrazite otpornosti bojila na promjene temperature i na utjecaj svjetlosti, što uzrokuje njihovo dugo zadržavanje u okolišu. Nadalje, sintetska bojila često su vrlo otporna na razgradnju pomoću mikroorganizama, što čini konvencionalne metode biološke obrade otpadnih voda neučinkovitima za uklanjanje/biorazgradnju bojila. Kao alternativa skupim konvencionalnim metodama uklanjanja bojila, poput koagulacije, flokulacije, oksidacije, ozoniranja ili membranskih procesa, razvijene su mnoge nove metode, uključujući i biološke metode uklanjanja pomoću mikroorganizama. Cilj ovog rada bio je preliminarno istražiti mogućnost upotrebe peleta gljive bijelog truljenja Trametes versicolor CCBAS AG 613 u svrhu obezbojenja (uklanjanja) sintetskog bojila malahitnog zelenila iz vodene otopine. Postotak uklanjanja bojila pomoću peleta nakon 24 h iznosio je preko 80 % u gotovo svim provedenim pokusima. Istražen je utjecaj početne koncentracije biomase, koncentracije malahitnog zelenila i dodatka glukoze na postotak uklanjanja bojila. Nadalje, istražena je mogućnost ponovnog korištenja peleta u ponovljivom šaržnom testu obezbojenja. Povećanjem početne koncentracije biomase došlo je do povećanja postotka uklanjanja bojila; dok je povećanje koncentracije bojila, neovisno o početnoj koncentraciji biomase, imalo suprotan učinak. Dodatak glukoze kao vanjskog izvora ugljika u vodenu otopinu bojila rezultirao je nešto manjim postotkom uklanjanja bojila. Ponovnim korištenjem u drugom ciklusu, peleti su zadržali sposobnost obezbojenja vodene otopine malahitnog zelenila, ali u nešto manjem postotku nego prilikom prvog korištenja. Preliminarni rezultati dobiveni ovim istraživanjem upućuju na mogućnost korištenja micelijskih peleta T. versicolor CCBAS AG 613 u bioremedijaciji obojenih otpadnih voda.Discharge of highly coloured effluents, containing different synthetic dyes, is considered to be an important source of surface and ground waters contamination. The presence of dyes in industrial effluents (namely wastewater) is a major concern due to their high thermal stability and photo stability, i.e. their persistence in the environment for an extended period of time. Furthermore, synthetic dyes exhibit recalcitrance towards removal/biodegradation by conventional biological wastewater treatment methods. Many alternative removal methods, including promising biological methods, have been developed towards highly cost conventional approaches such as coagulation-flocculation, oxidation or ozonation and membrane separation. The aim of this study was to preliminary investigate the dye (malachite green) decolourisation ability of white rot fungus Trametes versicolor CCBAS AG 613 mycelial pellets. Fungal pellets used could remove more than 80 % of the colour within 24 h. Effect of initial biomass concentration, malachite green concentration and glucose addition on dye percentage removal was tested. Furthermore, the longevity of this decolourisation activity was tested in repeated-batch mode. An increase in the initial biomass concentration positively affected the decolourisation, resulting in higher dye percentage removal. However, the increase in dye concentration had the opposite effect. Glucose addition to the dye solution resulted in slightly lower dye percentage removal. When used in repeated-batch mode, pellets exhibited lower dye percentage removal but still remained over 80 % after 24 h. These preliminary results indicate that white rot fungal pellets could effectively be used for bioremediation of coloured wastewater

Removal of synthetic dye malachite green using Trametes versicolor mycelial pellets

U ovom radu istražena je mogućnost upotrebe micelijskih peleta gljive bijelog truljenja Trametes versicolor CCBAS AG 613 s ciljem uklanjanja sintetskog bojila malahitnog zelenila iz vodene otopine. Istraživan je utjecaj početne koncentracije biomase, koncentracije malahitnog zelenila i dodatka glukoze u otopinu bojila na postotak uklanjanja bojila. U gotovo svim provedenim pokusima postotak uklanjanja bojila nakon 24 h iznosio je preko 80 %. Povećanjem početne koncentracije biomase došlo je do povećanja postotka uklanjanja bojila; dok je povećanje koncentracije bojila, neovisno o početnoj koncentraciji biomase, imalo suprotan učinak. Dodatak glukoze u vodenu otopinu bojila rezultirao je manjim postotkom uklanjanja bojila. Nadalje, istražena je mogućnost ponovnog korištenja peleta u drugom ciklusu. U ponovljenom testu peleti su zadržali sposobnost uklanjanja bojila iz vodene otopine malahitnog zelenila, ali u nešto manjem postotku nego prilikom prvog korištenja. Ovi preliminarni rezultati upućuju na mogućnost korištenja micelijskih peleta T. versicolor CCBAS AG613 za biološku obradu otpadnih voda obojenih malahitnim zelenilom.The aim of this study was to investigate the malachite green removal (decolourisation) ability of white rot fungus Trametes versicolor CCBAS AG613 mycelial pellets. The effect of initial biomass concentration, malachite green concentration and glucose addition on dye percentage removal was investigated. The dye percentage removal of more than 80% was achieved after 24 h in almost all runs. An increase in the initial biomass concentration positively affected the removal, resulting in higher dye percentage removal. However, the increase in dye concentration had the opposite effect. The addition of glucose to the dye solution resulted in slightly lower dye percentage removal. Furthermore, the longevity of pellets’ decolourisation activity was tested in repeated-batch mode. When used in repeated-batch mode, pellets still exhibited the malachite green decolourisation activity but to a slightly lower extent. These preliminary results indicate that T. versicolor CCBAS AG613 mycelial pellets could be effectively used for bioremediation of malachite green coloured wastewaters

Water for all : Proceedings of the 7th international scientific and professional conference Water for all

The 7th International Scientific and Professional Conference Water for all is organized to honour the World Water Day by the Josip Juraj Strossmayer University of Osijek, European Hygienic Engineering & Design Group (EHEDG), Danube Parks, Croatian Food Agency, Croatian Water, Faculty of Food Technology Osijek, Faculty of Agriculture in Osijek, Faculty of Civil Engineering Osijek, Josip Juraj Strossmayer University of Osijek Department of Biology, Josip Juraj Strossmayer University of Osijek Department of Chemistry, Nature Park “Kopački rit”, Osijek- Baranja County, Public Health Institute of the Osijek- Baranja County and „Vodovod-Osijek“ -water supply company in Osijek. The topic of World Water Day 2017 was "Wastewater" emphasizing the importance and influence of wastewater treatments on global environment. The international scientific and professional conference Water for all is a gathering of scientists and experts in the field of water management, including chemists, biologists, civil and agriculture engineers, with a goal to remind people about the significance of fresh water and to promote an interdisciplinary approach and sustainability for fresh water resource management. The Conference has been held since 2011. About 300 scientists and engineers submitted 95 abstracts to the 7th International Scientific and Professional Conference Water for all, out of which 33 was presented orally and 62 as posters. 47 full papers were accepted by the Scientific Committee. 38 full papers became the part of the this Proceedings while 9 papers were accepted for publication in Croatian Journal of Food Science and Technology and Electronic Journal of the Faculty of Civil Engineering Osijek - e-GFOS

Synthetic dyes decolorization of new Trametes fungus isolates

U ovom radu istražena je sposobnost obezbojenja sintetskih bojila (fuksin, kongo crvenilo i kristal violet) pomoću novih, do sada neistraženih izolata gljiva bijelog truljenja Trametes versicolor (L.) i Trametes gibbosa (Pers.) Fr. Istraživanjima sposobnosti obezbojenja prethodili su kvalitativni testovi za određivanje celulolitičke i lignolitičke aktivnosti koji su bili pozitivni za obje vrste gljiva. Gljive su uzgajane na agarnim pločama s dodatkom bojila u koncentracijama 10, 30, 50, 100 i 150 mg L$^{-1}$, te je kroz 14 dana praćen njihov porast i promjer obezbojenja. Nakon 14 dana, pri sobnoj temperaturi, nastavljeno je praćenje obezbojenje podloga do potpunog obezbojenja cijele agarne površine. Nakon 28 dana uzgoja gljiva T. versicolor TV8 je u potpunosti obezbojila cijelu površinu agarnih ploča s dodatkom kongo crvenila u svim odabranim koncentracijama te agarnih ploča s dodatkom fuksina i kristal violeta u svim koncentracijama, osim 150 mg L$^{-1}$. Gljiva T. gibbosa, potpuno je obezbojila cijelu površinu agarnih ploča s dodatkom kongo crvenila osim u najvećoj koncentraciji od 150 mg L$^{-1}$. U slučaju fuksina potpuno su obezbojene samo agarne ploče s dodatkom bojila u koncentracijama 10 i 30 mg L$^{-1}$, a ploče s dodatkom kristal violeta samo u koncentraciji od 10 mg L$^{-1}$. Nadalje, ispitana je mogućnost obezbojenja kongo crvenila pomoću T. versicolor u uvjetima fermentacije na čvrstim nosačima, pri čemu je kao supstrat i nosač korišten obojeni pivski trop. Tijekom uzgoja u trajanju od 21 dan, došlo je do porasta udjela proteina u uzorku te gubitka na masi supstrata od 17 % u odnosu na abiotičku kontrolu. Tijekom fermentacije na čvrstim nosačima došlo je do djelomičnog obezbojenja pivskog tropa što je potvrđeno vizualno i određivanjem promjene parametara boje.This study investigated the ability of newly isolated white rot fungi Trametes versicolor (L.) and Trametes gibbosa (Pers.) Fr. to decolorize synthetic dyes (fuchsine, congo red and crystal violet). Prior to decolorization tests, qualitative test for determination of cellulolytic and lignolytic activity were performed and were positive for both fungi. The fungi were cultivated on agar plates containing dyes at concentrations of 10, 30, 50, 100 and 150 mg L$^{-1}$ for 14 days. The radial growth and the zone of colour change on the agar plates were measured regularly every two days. The agar plates were then stored at room temperature and the change of colour was monitored visually for another 14 days. After 28 days, T. versicolor TV8 completely decolorize all agar plates containing congo red at all the applied concentrations. Apart from agar plates with the addition of 150 mg L$^{-1}$ fuchsine and crystal violet, all other agar plates with the adition of fuchsine and crystal violet were successfully decolorized. T. gibbosacompletely decolorized all agar plates containing congo red except at the highest concentration of 150 mg L$^{-1}$. Complete decolorization of agar plates containing fuchsine was only noted for concentrations of 10 and 30 mg L$^{-1}$, while in case of crystal violet the complete decolorization only occurred on agar plates with added concentration of crystal violet of 10 mg L$^{-1}$. Furthermore, T. versicolor was cultivated under solid-state conditions using congo red loaded brewers' spent grain as a carrier and substrate. T. versicolor grew readily under solid-state conditions, which was confirmed by the increase of protein content and substrate mass loss of 17% compared to abiotic control. Only partial decolorisation of congo red was observed, which was confirmed visually and by measuring the changes of color parameters

Decolourisation of synthetic dyes by fungal pellets

Obojene otpadne vode koje se ispuštaju u prirodne prijemnike, a sadrže velike koncentracije sintetskih bojila, važan su izvor onečišćenja površinskih i podzemnih voda. Prisutnost bojila u industrijskim otpadnim vodama predstavlja problem zbog izrazite otpornosti bojila na promjene temperature i na utjecaj svjetlosti, što uzrokuje njihovo dugo zadržavanje u okolišu. Nadalje, sintetska bojila često su vrlo otporna na razgradnju pomoću mikroorganizama, što čini konvencionalne metode biološke obrade otpadnih voda neučinkovitima za uklanjanje/biorazgradnju bojila. Kao alternativa skupim konvencionalnim metodama uklanjanja bojila, poput koagulacije, flokulacije, oksidacije, ozoniranja ili membranskih procesa, razvijene su mnoge nove metode, uključujući i biološke metode uklanjanja pomoću mikroorganizama. Cilj ovog rada bio je preliminarno istražiti mogućnost upotrebe peleta gljive bijelog truljenja Trametes versicolor CCBAS AG 613 u svrhu obezbojenja (uklanjanja) sintetskog bojila malahitnog zelenila iz vodene otopine. Postotak uklanjanja bojila pomoću peleta nakon 24 h iznosio je preko 80 % u gotovo svim provedenim pokusima. Istražen je utjecaj početne koncentracije biomase, koncentracije malahitnog zelenila i dodatka glukoze na postotak uklanjanja bojila. Nadalje, istražena je mogućnost ponovnog korištenja peleta u ponovljivom šaržnom testu obezbojenja. Povećanjem početne koncentracije biomase došlo je do povećanja postotka uklanjanja bojila; dok je povećanje koncentracije bojila, neovisno o početnoj koncentraciji biomase, imalo suprotan učinak. Dodatak glukoze kao vanjskog izvora ugljika u vodenu otopinu bojila rezultirao je nešto manjim postotkom uklanjanja bojila. Ponovnim korištenjem u drugom ciklusu, peleti su zadržali sposobnost obezbojenja vodene otopine malahitnog zelenila, ali u nešto manjem postotku nego prilikom prvog korištenja. Preliminarni rezultati dobiveni ovim istraživanjem upućuju na mogućnost korištenja micelijskih peleta T. versicolor CCBAS AG 613 u bioremedijaciji obojenih otpadnih voda.Discharge of highly coloured effluents, containing different synthetic dyes, is considered to be an important source of surface and ground waters contamination. The presence of dyes in industrial effluents (namely wastewater) is a major concern due to their high thermal stability and photo stability, i.e. their persistence in the environment for an extended period of time. Furthermore, synthetic dyes exhibit recalcitrance towards removal/biodegradation by conventional biological wastewater treatment methods. Many alternative removal methods, including promising biological methods, have been developed towards highly cost conventional approaches such as coagulation-flocculation, oxidation or ozonation and membrane separation. The aim of this study was to preliminary investigate the dye (malachite green) decolourisation ability of white rot fungus Trametes versicolor CCBAS AG 613 mycelial pellets. Fungal pellets used could remove more than 80 % of the colour within 24 h. Effect of initial biomass concentration, malachite green concentration and glucose addition on dye percentage removal was tested. Furthermore, the longevity of this decolourisation activity was tested in repeated-batch mode. An increase in the initial biomass concentration positively affected the decolourisation, resulting in higher dye percentage removal. However, the increase in dye concentration had the opposite effect. Glucose addition to the dye solution resulted in slightly lower dye percentage removal. When used in repeated-batch mode, pellets exhibited lower dye percentage removal but still remained over 80 % after 24 h. These preliminary results indicate that white rot fungal pellets could effectively be used for bioremediation of coloured wastewater