The largest bright ULX population in a galaxy: X-ray variability and Luminosity Function in the Cartwheel ring Galaxy

We analyse all the available Chandra observations of the Cartwheel Galaxy and its compact group, taken between 2001 and 2008, with the main aim of addressing the variability in the X-ray band for this spectacular collisional ring galaxy. We focus on the study of point-like sources, in particular we are interested in Ultraluminous X-ray sources (ULXs, Lx >= 10^39 erg/s), that we treat as a class. We exploit archival XMM-Newton data to enrich the study of the long-term variability, on timescales of months to years. We find a total of 44 sources in the group area, of which 37 in total are ULXs positionally linked with the galaxies and of which we can study variability. They are 29 in the Cartwheel itself, 7 in G1 and 1 in G3. About one third of these 37 sources show long-term variability, while no variability is detected within the single observations. Of those, 5 ULXs have a transient behaviour with a maximum range of variability (Lmax/Lmin) of about one order of magnitude and are the best candidate neutron stars. The X-ray Luminosity Function (XLF) of the point-like sources remains consistent in shape between the Chandra observations both for the Cartwheel galaxy itself and for G1, suggesting that flux variability does not strongly influence the average properties of the population on the observation timescales.Comment: 17 pages, 5 figures, Accepted for publication in MNRA

Assessing Crop Water Requirement and Yield by Combining ERA5-Land Reanalysis Data with CM-SAF Satellite-Based Radiation Data and Sentinel-2 Satellite Imagery

The widespread development of Earth Observation (EO) systems and advances in numerical atmospheric modeling have made it possible to use the newest data sources as input for crop–water balance models, thereby improving the crop water requirements (CWR) and yield estimates from the field to the regional scale. Satellite imagery and numerical weather prediction outputs offer high resolution (in time and space) gridded data that can compensate for the paucity of crop parameter field measurements and ground weather observations, as required for assessments of CWR and yield. In this study, the AquaCrop model was used to assess CWR and yield of tomato on a farm in Southern Italy by assimilating Sentinel-2 (S2) canopy cover imagery and using CM-SAF satellite-based radiation data and ERA5-Land reanalysis as forcing weather data. The prediction accuracy was evaluated with field data collected during the irrigation season (April–July) of 2021. Satellite estimates of canopy cover differed from ground observations, with a RMSE of about 11%. CWR and yield predictions were compared with actual data regarding irrigation volumes and harvested yield. The results showed that S2 estimates of crop parameters represent added value, since their assimilation into crop growth models improved CWR and yield estimates. Reliable CWR and yield estimates can be achieved by combining the ERA5-Land and CM-SAF weather databases with S2 imagery for assimilation into the AquaCrop model

The Role of Metformin in the Management of NAFLD

Nonalcoholic fatty liver disease (NAFLD) is the most common liver disorder worldwide. Its prevalence ranges 10–24% in the general population, reaching 60–95% and 28–55% in obese and diabetic patients, respectively. Although the etiology of NAFLD is still unclear, several lines of evidences have indicated a pathogenetic role of insulin resistance in this disorder. This concept has stimulated several clinical studies where antidiabetic drugs, such as insulin sensitizers including metformin, have been evaluated in insulin-resistant, NAFLD patients. These studies indicate that metformin might be of benefit in the treatment of NAFLD, also in nondiabetic patients, when associated to hypocaloric diet and weight control. However, the heterogeneity of these studies still prevents us from reaching firm conclusions about treatment guidelines. Moreover, metformin could have beneficial tissue-specific effects in NAFLD patients irrespective of its effects as insulin sensitizer

Extremophilic bacteria restrict the growth of Macrophomina phaseolina by combined secretion of polyamines and lytic enzymes

[EN] Extremophilic microorganisms were screened as biocontrol agents against two strains of Macrophomina phaseolina (Mp02 and 06). Stenotrophomonas sp. AG3 and Exiguobacterium sp. S58 exhibited a potential in vitro antifungal effect on Mp02 growth, corresponding to 52.2% and 40.7% inhibition, respectively. This effect was confirmed by scanning electron microscopy, where images revealed marked morphological alterations in fungus hyphae. The bacteria were found to secrete lytic enzymes and polyamines. Exiguobacterium sp. S56a was the only strain able to reduce the growth of the two strains of M. phaseolina through their supernatant. Antifungal supernatant activity was correlated with the ability of bacteria to synthesize and excrete putrescine, and the exogenous application of this polyamine to the medium phenocopied the bacterial antifungal effects. We propose that the combined secretion of putrescine, spermidine, and lytic enzymes by extremophilic microorganism predispose these microorganisms to reduce the disease severity occasioned by M. phaseolina in soybean seedlings.The authors acknowledge the generous financial support by the PICT V Bicentenario 2010 1788 Project (FONCyT, Argentina). This work was performed in the context of a project called ¿Análisis de Adaptación al Cambio Climático en Humedales Andinos¿. ID: 6188775¿8-LP13. Ministerio del Medio Ambiente, Región de Antofagasta. We are also grateful to Lic. C. Pérez Brandan for providing us with the M. phaseolina strains used in this study.Santos, AP.; Nieva Muratore, L.; Sole-Gil, A.; Farías, ME.; Ferrando Monleón, AR.; Blazquez Rodriguez, MA.; Belfiore, C. (2021). Extremophilic bacteria restrict the growth of Macrophomina phaseolina by combined secretion of polyamines and lytic enzymes. Plant Biotechnology Reports. 32:1-9. https://doi.org/10.1016/j.btre.2021.e00674S193

SARS-CoV-2 Infection as a Determining Factor to the Precipitation of Ischemic Priapism in a Young Patient with Asymptomatic COVID-19

COVID-19 is a disease characterized by respiratory distress, systemic inflammation, multiple organ dysfunction and coagulation disorders, chiefly pulmonary embolism, and deep venous thrombosis. In this case report, we discuss a peculiar case of ischemic priapism in a 36-year-old patient with asymptomatic COVID-19 and no other plausible causes of thrombophilia and/or alternative causes of priapism, as well as discussing possible explanations for such remarkable findings and comparing them to analogous cases recorded in literature. The patient was unsuccessfully treated via cavernous blood aspiration and required several shunting procedures, with no further recurrences and negative testing for pulmonary embolism, deep venous thrombosis, and other causes of thrombophilia

Circulating CRP Levels Are Associated with Epicardial and Visceral Fat Depots in Women with Metabolic Syndrome Criteria

Sexual dimorphism accounts for significant differences in adipose tissue mass and distribution. However, how the crosstalk between visceral and ectopic fat depots occurs and which are the determinants of ectopic fat expansion and dysfunction remains unknown. Here, we focused on the impact of gender in the crosstalk between visceral and epicardial fat depots and the role of adipocytokines and high-sensitivity C-reactive protein (hs-CRP). A total of 141 outward patients (both men and women) with one or more defining criteria for metabolic syndrome (MetS) were consecutively enrolled. For all patients, demographic and clinical data were collected and ultrasound assessment of visceral adipose tissue (VFth) and epicardial fat (EFth) thickness was performed. Hs-CRP and adipocytokine levels were assessed by enzyme-linked immunosorbent assay (ELISA). Men were characterized by increased VFth and EFth (p-value < 0.001 and 0.014, respectively), whereas women showed higher levels of adiponectin and leptin (p-value < 0.001 for both). However, only in women VFth and EFth significantly correlated between them (p = 0.013) and also with leptin (p < 0.001 for both) and hs-CRP (p = 0.005 and p = 0.028, respectively). Linear regression confirmed an independent association of both leptin and hs-CRP with VFth in women, also after adjustment for age and MetS (p = 0.012 and 0.007, respectively). In conclusion, men and women present differences in epicardial fat deposition and systemic inflammation. An intriguing association between visceral/epicardial fat depots and chronic low-grade inflammation also emerged. In women Although a further validation in larger studies is needed, these findings suggest a critical role of sex in stratification of obese/dysmetabolic patients

Pre-supernova stellar feedback in nearby starburst dwarf galaxies

Context. Stellar feedback in dwarf galaxies remains, to date, poorly explored, yet is crucial to understanding galaxy evolution in the early Universe. In particular, pre-supernova feedback has recently been found to play a significant role in regulating and disrupting star formation in larger spiral galaxies, but it remains uncertain if it also plays this role in dwarfs.Aims. We study the ionised gas properties and stellar content of individual star-forming regions across three nearby, low-metallicity (12 + log(O/H)∼7.5), dwarf (M* ∼ 40 × 106 M⊙), starburst (log(SFR)∼ − 2.8) galaxies (J0921, KKH046, and Leo P) to investigate how massive stars influence their surroundings and how this influence changes as a function of environment.Methods. We extracted integrated spectra of 30 HII regions from archival VLT/MUSE integral field spectroscopic observations of these three dwarf starburst galaxies. We fitted the HII regions’ main emission lines with Gaussian profiles to derive their oxygen abundances, electron densities, and luminosities, and we used the Stochastically Lighting Up Galaxies (SLUG) code to derive the stellar mass, age, and bolometric luminosity of the stellar populations driving the HII regions. We quantified two pre-supernova stellar feedback mechanisms, namely the direct radiation pressure and photoionisation feedback, and explored how feedback strength varies with HII region properties.Results. Our findings suggest that stellar feedback has less of an impact on evolved regions, with both the pressure of the ionised gas and the direct radiation pressure decreasing as a function of HII region size (i.e. the evolutionary stage). We find that these stellar feedback mechanisms are also dependent on the metallicity of the HII regions. These findings extend results from stellar feedback studies of more massive star-forming galaxies to the low-mass, low-metallicity regime. In addition, we conclude that the use of stochastic stellar population models significantly affects the relationships found between feedback-related pressure terms and HII region properties, and in particular that non-stochastic models can severely underestimate the bolometric luminosity of low-mass stellar populations

IGF-I induces upregulation of DDR1 collagen receptor in breast cancer cells by suppressing MIR-199a-5p through the PI3K/AKT pathway.

Discoidin Domain Receptor 1 (DDR1) is a collagen receptor tyrosine-kinase that contributes to epithelial-to-mesenchymal transition and enhances cancer progression. Our previous data indicate that, in breast cancer cells, DDR1 interacts with IGF-1R and positively modulates IGF-1R expression and biological responses, suggesting that the DDR1-IGF-IR cross-talk may play an important role in cancer.In this study, we set out to evaluate whether IGF-I stimulation may affect DDR1 expression. Indeed, in breast cancer cells (MCF-7 and MDA-MB-231) IGF-I induced significant increase of DDR1 protein expression, in a time and dose dependent manner. However, we did not observe parallel changes in DDR1 mRNA. DDR1 upregulation required the activation of the PI3K/AKT pathway while the ERK1/2, the p70/mTOR and the PKC pathways were not involved. Moreover, we observed that DDR1 protein upregulation was induced by translational mechanisms involving miR-199a-5p suppression through PI3K/AKT activation. This effect was confirmed by both IGF-II produced by cancer-associated fibroblasts from human breast cancer and by stable transfection of breast cancer cells with a human IGF-II expression construct. Transfection with a constitutively active form of AKT was sufficient to decrease miR-199a-5p and upregulate DDR1. Accordingly, IGF-I-induced DDR1 upregulation was inhibited by transfection with pre-miR-199a-5p, which also impaired AKT activation and cell migration and proliferation in response to IGF-I.These results demonstrate that, in breast cancer cells, a novel pathway involving AKT/miR-199a-5p/DDR1 plays a role in modulating IGFs biological responses. Therefore, this signaling pathway may represent an important target for breast cancers with over-activation of the IGF-IR axis

Malignant hypertension and hyperreninemia: primary or secondary hypertension? A case report

Malignant hypertension is a rare condition characterized by severe hypertension and multi-organ ischemic damage. Marked activation of the renin-angiotensin system is observed in many patients, but its persistence over time is not known. We report a case of a 42-year-old woman who presented with severe hypertension and multi-organ damage. Initial evaluation showed an elevated value of direct renin concentration with normal plasma aldosterone concentration and a nodular lesion in the left adrenal gland. The differential diagnosis between the primary and secondary form of hypertension had to be questioned. Consequently, the patient was followed up for 20 months. Repeated checks showed a significant increase in renin levels with a normal aldosterone concentration and regression of organ damage. After 20 months, renin values returned within normal range. Hyperreninemia persisting over a long period of time has not been fully explained. Long-term follow-up allowed us to attribute malignant hypertension to de novo essential hypertension

The Effects of Angiotensin II or Angiotensin 1-7 on Rat Pial Microcirculation during Hypoperfusion and Reperfusion Injury: Role of Redox Stress

Renin-angiotensin systems produce angiotensin II (Ang II) and angiotensin 1-7 (Ang 1-7), which are able to induce opposite effects on circulation. This study in vivo assessed the effects induced by Ang II or Ang 1-7 on rat pial microcirculation during hypoperfusion-reperfusion, clarifying the mechanisms causing the imbalance between Ang II and Ang 1-7. The fluorescence microscopy was used to quantify the microvascular parameters. Hypoperfusion and reperfusion caused vasoconstriction, disruption of blood-brain barrier, reduction of capillary perfusion and an increase in reactive oxygen species production. Rats treated with Ang II showed exacerbated microvascular damage with stronger vasoconstriction compared to hypoperfused rats, a further increase in leakage, higher decrease in capillary perfusion and marker oxidative stress. Candesartan cilexetil (specific Ang II type 1 receptor (AT1R) antagonist) administration prior to Ang II prevented the effects induced by Ang II, blunting the hypoperfusion-reperfusion injury. Ang 1-7 or ACE2 activator administration, preserved the pial microcirculation from hypoperfusion-reperfusion damage. These effects of Ang 1-7 were blunted by a Mas (Mas oncogene-encoded protein) receptor antagonist, while Ang II type 2 receptor antagonists did not affect Ang 1-7-induced changes. In conclusion, Ang II and Ang 1-7 triggered different mechanisms through AT1R or MAS receptors able to affect cerebral microvascular injury