Distinction among the puparia of three blowfly species (Diptera: Calliphoridae) frequently found on unburied corpses

Calliphorid larvae are important in the decomposition of carrion. Since these larvae are present in the primary stages of succession on carcasses, they may be important indicators of death time and the movement of corpses in homicide investigations. In this study we examined the morphological differences among puparia of Chrysomya megacephala, C. putoria and Cochliomyia macellaria. Puparia of the three species (N=30, each) were obtained from the F2 generation bred in culture medium at 25° C, and 60% relative humidity on a 12 h photoperiod. The interspecific differences found were related to the conspicuousness of six tubercles located in the region near the posterior spiracles and to the distance between the two peritrema involving the spiracles. The latter were (mean ± SD) 15.2 ± 3.1 mum for C. megacephala, 18.8 ± 2.8 mum for C. putoria and 16.5 ± 3.5 mum for C. macellaria. The results of the present study may be useful in forensic entomology.78178

Middle eastern genetic legacy in the paternal and maternal gene pools of Chuetas

Chuetas are a group of descendants of Majorcan Crypto-Jews (Balearic Islands, Spain) who were socially stigmatized and segregated by their Majorcan neighbours until recently; generating a community that, although after the seventeenth century no longer contained Judaic religious elements, maintained strong group cohesion, Jewishness consciousness, and endogamy. Collective memory fixed 15 surnames as a most important defining element of Chueta families. Previous studies demonstrated Chuetas were a differentiated population, with a considerable proportion of their original genetic make-up. Genetic data of Y-chromosome polymorphism and mtDNA control region showed, in Chuetas’ paternal lineages, high prevalence of haplogroups J2-M172 (33%) and J1-M267 (18%). In maternal lineages, the Chuetas hallmark is the presence of a new sub-branching of the rare haplogroup R0a2m as their modal haplogroup (21%). Genetic diversity in both Y-chromosome and mtDNA indicates the Chueta community has managed to avoid the expected heterogeneity decrease in their gene pool after centuries of isolation and inbreeding. Moreover, the composition of their uniparentally transmitted lineages demonstrates a remarkable signature of Middle Eastern ancestry—despite some degree of host admixture—confirming Chuetas have retained over the centuries a considerable degree of ancestral genetic signature along with the cultural memory of their Jewish origin.This work was partially supported by grant AAEE246/2014 from the Direcció General de R + D + I (Comu-nitat Autònoma de les Illes Balears) and European Regional Development Fund (ERDF) and FCT, Portuguese Foundation for Science and Tecnology PTDC/ATP-DEM/4545/2012 project financed by European Social Funds (COMPETE-FEDER). We are grateful to Maria Trinidad Garcia (from the Serveis Cientificotècnics of the Uni-versitat de les Illes Balears) for her assistance and help with capillary electrophoresis procedures, María Luz Gómez-Barbeito for her help in mtDNA sequencing and Meryl Wyn Jones for the English language corrections. We would also like to sincerely thank all the people who volunteered to participate in this study

A comparison of research data management platforms: architecture, flexible metadata and interoperability

Research data management is rapidly becoming a regular concern for researchers, and institutions need to provide them with platforms to support data organization and preparation for publication. Some institutions have adopted institutional repositories as the basis for data deposit, whereas others are experimenting with richer environments for data description, in spite of the diversity of existing workflows. This paper is a synthetic overview of current platforms that can be used for data management purposes. Adopting a pragmatic view on data management, the paper focuses on solutions that can be adopted in the long tail of science, where investments in tools and manpower are modest. First, a broad set of data management platforms is presented-some designed for institutional repositories and digital libraries-to select a short list of the more promising ones for data management. These platforms are compared considering their architecture, support for metadata, existing programming interfaces, as well as their search mechanisms and community acceptance. In this process, the stakeholders' requirements are also taken into account. The results show that there is still plenty of room for improvement, mainly regarding the specificity of data description in different domains, as well as the potential for integration of the data management platforms with existing research management tools. Nevertheless, depending on the context, some platforms can meet all or part of the stakeholders' requirements

AGRONOMIC AND MOLECULAR CHARACTERIZATION OF DIPLOID IMPROVED BANANA GENOTYPES

An investigation about the genetical diversity among eleven banana diploid genotypes using nine agronomical characteristics and sixteen microsatellite markers was implanted at Embrapa Cassava and Tropical Fruits. Cruz das Almas (BA), Brazil. The generalized distance of Mahalanobis indicated the presence of genetic diversity. The genotypes were grouped into tree clusters. Among the investigated characteristics, the plant height, number of bunch's, number of fruits per bunch and pseudostem exhibited high contribution towards genetic divergence. The average number of alleles per primer was 7.51, with a total of 120 alleles identified. The average similarity among the all diploid was 0.44, range from 0.29 up to 0.60. New parental combinations can be identified with base of the divergence between these diploids, contributing for development of new improved diploids preventing the narrow genetic base and creating new genetic variability for selection.31115416

Acute onset of intracranial subdural hemorrhage five days after spinal anesthesia for knee arthroscopic surgery: a case report

<p>Abstract</p> <p>Introduction</p> <p>Spinal anesthesia is a widely used general purpose anesthesia. However, serious complications, such as intracranial subdural hemorrhage, can rarely occur.</p> <p>Case presentation</p> <p>We report the case of a 73-year-old Japanese woman who had acute onset of intracranial subdural hemorrhage five days after spinal anesthesia for knee arthroscopic surgery.</p> <p>Conclusion</p> <p>This case highlights the need to pay attention to acute intracranial subdural hemorrhage as a complication after spinal anesthesia. If the headache persists even in a supine position or nausea occurs abruptly, computed tomography or magnetic resonance imaging of the brain should be conducted. An intracranial subdural hematoma may have a serious outcome and is an important differential diagnosis for headache after spinal anesthesia.</p