FA-6 Analysis of Sperm Freezing Capability of Various Bulls at the Singosari AI Center

Artificial insemination (AI) has been widely applied to improve genetic quality in cattle worldwide. One of the most important factors in AI program is the quality of semen.  Many procedures in semen processing have been developed to preserve the sperm quality. The Singosari AI center produces the frozen semen through cryopreservation method. During this process, the number of semen collected will be rejected if the quality is low. Fresh semen with less than 70% of sperm motility, before freezing semen with less than 55%of sperm motility and less than 40% of sperm post-thawing motility evaluation will be rejected, so that it was only the viable sperm will be processed to be frozen semen commercial. Therefore, comparative study was carried out to analyze the freezing capability of sperm

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring

Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm’s productivity as a provider of meat sources. This study aimed to determine the sex ratio of the offspring of cows inseminated with Y‐bearing sperm enriched by Percoll density gradient centrifugation and swim‐up, combined with delayed fixed‐time artificial insemination (FTAI). Materials and Methods: Ejaculates of Simmental bulls were divided into four equal portions and grouped as T0 (control, non‐sexed semen), T1 and T2 were sexed semen using Percoll density gradient centrifugation three and five levels, respectively, and T3 was sexed semen using swim‐up. After the sex was sorted, the semen was diluted in a tris‐egg yolk extender, packaged in French mini‐straws containing 50 million live sperm cells, and frozen. Pre‐sexed, post‐sexed, and post‐thawed spermatozoa were evaluated based on progressive motility, viability, intact plasma membrane, and abnormality. The post‐thawed semen of T0 was artificially inseminated to recipient cows at 12 h after onset of estrus (not delayed FTAI). Meanwhile, the delayed FTAI was conducted 18‐20 h after onset of estrus using the T0, the best of T1 and T2, and the T3 post‐thawed semen. Results: The Percoll density gradient centrifugation reduced motility, viability, and intact plasma membrane but increased sperm abnormalities. Meanwhile, the swim‐up process increased motility, viability, and intact plasma membrane of sperm cells but decreased sperm abnormalities. Post‐thawed semen decreased motility, viability, and intact plasma membrane of sperm cells but increased sperm abnormalities. The sex ratio of the Simmental crossbred offspring was 96.08% and 100% in T1 and T3, respectively, compared to 48.25% and 67.39% in T0 not delayed and delayed FTAI, respectively. Conclusion: The Percoll density gradient centrifugation and swim‐up methods are prospective for obtaining male offspring. Keywords: agricultural innovation, farm productivity, motility, pregnancy rate, sperm morphologic abnormality, viabilit

Effect of Addition of Green Tea Extract (Camellia sinensis) in Egg Yolk Tris Diluter on Spermatozoa Quality in Bali Cattle (Bos sondaicus) After Freezing

This study aimed to determine the effect of adding 96% ethanol extract of green tea (Camellia sinensis) in egg yolk tris diluter on the quality of spermatozoa of Bali cattle (Bos sondaicus) after freezing. The samples were divided into four different treatment groups, which contained semen sample + 13% glycerol + each egg yolk tris diluter + green tea extract (P0) 0 mg/100 ml, (P1) 0,05 mg/100 ml, ( P2) 0,10 mg/100 ml, and (P3) 0,15 mg/100 ml. The variables observed included motility, viability, and spermatozoa abnormalities. Data were analyzed using OneWay ANOVA and continued with Duncan's test (p<0,05). The results showed that the P3 group had motility of 49,00 ± 2,15, viability of 61,92 ± 1,70, and abnormality of 4,24 ± 2,16 significantly compared to the other groups. In conclusion, the P3 group with the addition of 0,15 mg/100 ml of green tea could increase the motility, viability, and abnormal spermatozoa of Bali cattle

Pengaruh Medroksi Progesteron Asetat Terhadap Berat Badan Dan Kadar Kolesterol - HDL Darah Tikus Putih Jantan (Rattus Norvegicus)

Penelitian ini bertujuan untuk mengetahui pengaruh Medroksi Progesteron Asetat (MPA) sebagai alat kontrasepsi terhadap berat badan dan kadar kolesteroi-HDL darah tikus putih jantan. Hewan percobaan yang digunakan sebanyak dua puluh empat ekor tikus putih jantan yang berumur ± 5 - 6 bulan dengan berat badan rataan 257,96 ± 32,51 gram. Selama percobaan, tikus putih jantan tersebut diberi pakan ayam buatan PT. COMFEED INDONESIA berbentuk pellet. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan empat perlakuan yang masing-masing perlakuan terdiri dari enam ulangan. MPA diberikan secara intramuskuler sebanyak 3 kali dengan selang waktu pemberian 28 hari. Pada perlakuan A (P A) tanpa pemberian MPA sebagai kontrol, perlakuan B (Pe) pemberian MPA dengan dosis 4 mg, perlakuan C (Pc) pemberian MPA dengan dosis 8 mg dan perlakuan D (Po) pemberian MPA dengan dosis 12 mg. Penimbangan berat badan dilakukan pada saat sebelum perlakuan dan setiap dua minggu saat perlakuan diberikan, sedangkan pemeriksaan kadar kolesteroi-HDL dilakukan dua minggu setelah pemberian MPA yang terakhir. Hasil penelitian menunjukkan bahwa MPA yang diberikan secara intramuskuler dengan dosis 0 mg, 4 mg, 8 mg, 12 mg sebanyak tiga kali dengan selang waktu pemberian 28 hari tidak menimbulkan pengaruh yang nyata (p > 0,05) terhadap berat badan dan kadar kolesteroi-HDL darah tikus putih jantan

PENGARUH MEDROKSI PROGESTERON ASETAT TERHADAP SERAT BADAN DAN KADAR KOLESTEROL HDL DARAH TIKUS PUTIH JANTAN (Rattus norvegicus)

Penelitian ini bertujuan untuk mengetahui pengaruh Medroksi Progesteron Asetat (MPA) sebagai alat kontrasepsi terhadap berat badan dan kadar kolesterol-HDL darah tikus putih jantan. Hewan percobaan yang digunakan sebanyak dua puluh empat ekor tikus putih jantan yang berumur ± 5 -6 bulan dengan berat badan rataan 257,96 ± 32,51 gram. Selama percobaan, tikus putih jantan tersebut diberi pakan ayam buatan PT. COMFEED INDONESIA berbentuk pellet. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan empat perlakuan yang masing-masing perlakuan terdiri dari enam ulangan. MPA diberikan secara intramuskuler sebanyak 3 kali dengan selang waktu pemberian 28 hari. Pada perlakuan A (PN tanpa pemberian MPA sebagai kontrol, perlakuan B (Pe) pemberian MPA dengan dosis 4 mg. perlakuan C (Pc) pemberian MPA dengan dosis 8 mg dan perlakuan D (Po) pemberian MPA dengan dosis 12 mg. Penimbangan berat badan dilakukan pada saat sebelum perlakuan dan setiap dua minggu saat perlakuan diberikan, sedangkan pemeriksaan kadar kolesterol-HDL dilakukan dua minggu setelah pemberian MPA yang terakhir. Hasil penelitian menunjukkan bahwa MPA yang diberikan secara intramuskuler dengan dosis 0 mg. 4 mg, 8 mg, 12 mg sebanyak tiga kali dengan selang waktu pemberian 28 hari tidak menimbulkan pengaruh yang nyata (p > 0,05) terhadap be rat badan dan kadar kolesterol-HDL darah tikus putih jantan

KUALITAS, EKSPRESI HSP 70 DAN KERUSAKAN DNA SPERMATOZOA POST THAWING SAPI LIMOSIN PASCA PENDINGINAN PADA SUHU 5°C

Kualitas semen beku merupakan salah satu faktor penunjang keberhasilan inseminasi buatan. Untuk mendapatkan fertilitas yang tinggi maka spermatozoa setelah ditambahkan pengencer harus didinginkan pada suhu 5°C. Penelitian ini bertujuan untuk mengetahui kualitas (motilitas, viabilitas, abnormalitas dan membran plasma utuh), ekspresi HSP 70 dan kerusakan DNA spermatozoa post thawing pasca pendinginan pada suhu 5°C selama 4 dan 22 jam dari satu ekor pejantan Limosin. Semen ditampung seminggu dua kali dengan menggunakan vagina buatan, kemudian dilakukan evaluasi makroskopis dan mikroskopis termasuk viabilitas, abnormalitas dan membran plasma utuh. Kemudian didinginkan pada pada suhu 5°C selama 4 dan 22 jam kemudian dilakukan evaluasi kualitas (motilitas, viabilitas, abnormalitas dan Membran Plasma Utuh sel), ekspresi HSP 70 dan kerusakan DNA spermatozoa before freezing dan post thawing. Hasil penelitian menunjukkan bahwa motilitas spermatozoa before freezing pasca pendinginan pada suhu 5°C selama 22 jam (58,8 ± 2,3) berbeda nyata (p<0,05) dengan 4 jam (55,6 ± 1,8). Tetapi tidak berbeda nyata pada viabilitas, abnormalitas, membran plasma utuh, ekspresi HSP 70 dan kerusakan DNA spermatozoa . Pada post thawing, lama pendinginan pada suhu 5°C tidak berbeda nyata (p˃0,05) pada kualitas (motilitas, viabilitas, abnormalitas dan membran plasma utuh), ekspresi HSP 70 dan kerusakan DNA spermatozoa

The Influence of Individual Factors on the Characteristic and Production of Frozen Semen of Bali Cattle

Individual difference is one of the factors that influence the semen quality during ejaculation. The study aimed to analyze the influence of individual factors on the characteristic and production of frozen semen of Bali cattle.&nbsp; One hundred fifteen ejaculate semen samples were collected from 10 Bali cattle using an artificial vagina. The semen color, consistency, pH, volume, abnormality, motility, concentration, total sperm (TS), and total motile sperm (TMS) were analyzed. The frozen semen was evaluated for motility before freezing (MBF), post thawing motility (PTM), total motile sperm form PTM (TSMP), and frozen semen production. Data were analyzed statistically using one-way analyzed variance to obtain the difference between individuals. The results showed the semen was a milky color with a thin, medium, and thick consistency. The semen pH and motility were not significantly different between individuals (p&gt;0.05). In comparison, the individual differences affected (p&gt;0.01) on volume, abnormality, concentration, TS, and TMS. The individual differences of frozen semen also affected MBF, PTM, and TSMP. The characteristics and production of frozen semen were influenced by individual differences, which can then be used to determine superior males