Additional file 3: of Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation

OPC maturation and recruitment is not impaired in non-lesioned areas during progressive EAE in GFAPγR1Δ mice. Longitudinal spinal cord sections from WT and GFAPγR1Δ mice, treated with isotype control or anti-TNF mAb, were stained for OLG and OPC during acute (d19) and chronic (d30) EAE. A. Quantification of differentiated myelinating OLG (CC1+Olig2+) per square millimeter non-lesioned area. D. Quantification of OPCs (CC1−Olig2+) per square millimeter non-lesioned area. Data represent the mean ± SEM of five to seven separate fields per mouse with two to three mice per group from two independent experiments. P values were determined by Wilcoxon rank sum-test. GFAPγR1Δ in all panels represents GFAPγR1Δ mice treated with isotype control mAb. (TIF 153 kb

Additional file 1: of Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation

TNF blockade reduces astrocyte and myeloid cell reactivity during progressive EAE in GFAPγR1Δ mice. Longitudinal spinal cord sections from WT and GFAPγR1Δ mice treated with isotype control or anti-TNF mAb were stained for astrocyte (GFAP) or myeloid cell (Iba-1) reactivity at d19 and d30 as indicated. Data represents three to four separate fields per mouse with two to three mice per group. (TIF 1843 kb

Additional file 2: of Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation

TNF neutralization limits OLG and T cell apoptosis during progressive EAE in GFAPγR1Δ mice. Longitudinal spinal cord sections from WT and GFAPγR1Δ mice, treated with isotype control or anti-TNF mAb, were stained for apoptosis during acute (d19) and chronic (d30) EAE. A. Quantification of apoptotic mature OLG (TUNEL+ CC1+ Olig2−) per square millimeter non-lesioned area. C. Quantification of apoptotic T cells (TUNEL+ CD3+) per square millimeter lesion area. Data represent mean ± SEM of two to three separate fields per mouse with two to three mice per group from two independent experiments. P values were determined by Student’s t test. GFAPγR1Δ in all panels represents GFAPγR1Δ mice treated with isotype control mAb. (TIF 133 kb