Additional file 2: of Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation
TNF neutralization limits OLG and T cell apoptosis during progressive EAE in GFAPγR1Δ mice. Longitudinal spinal cord sections from WT and GFAPγR1Δ mice, treated with isotype control or anti-TNF mAb, were stained for apoptosis during acute (d19) and chronic (d30) EAE. A. Quantification of apoptotic mature OLG (TUNEL+ CC1+ Olig2−) per square millimeter non-lesioned area. C. Quantification of apoptotic T cells (TUNEL+ CD3+) per square millimeter lesion area. Data represent mean ± SEM of two to three separate fields per mouse with two to three mice per group from two independent experiments. P values were determined by Student’s t test. GFAPγR1Δ in all panels represents GFAPγR1Δ mice treated with isotype control mAb. (TIF 133 kb
