Diversity of actin architecture in human osteoclasts: Network of curved and branched actin supporting cell shape and intercellular micrometer level tubes

Osteoclasts are multinucleated bone-resorbing cells with a dynamic actin cytoskeleton. Osteoclasts are derived from circulating mononuclear precursors. Confocal and stimulated emission depletion (STED) super-resolution microscopy was used to investigate peripheral blood-derived human osteoclasts cultured on glass surfaces. STED and confocal microscopy demonstrated that the actin was curved and branched, for instance, in the vicinity of membrane ruffles. The overall architecture of the curved actin network extended from the podosomes to the top of the cell. The other novel finding was that a micrometer-level tube containing actin bridged the osteoclasts well above the level of the culture glass. The actin filaments of the tubes originated from the network of curved actin often surrounding a group of nuclei. Furthermore, nuclei were occasionally located inside the tubes. Our findings demonstrated the accumulation of c-Src, cortactin, cofilin, and actin around nuclei suggesting their role in nuclear processes such as the locomotion of nuclei. ARP2/3 labeling was abundant at the substratum level of osteoclasts and in the branched actin network, where it localized to the branching points. We speculate that the actin-containing tubes of osteoclasts may provide a means of transportation of nuclei, e.g., during the fusion of osteoclasts. These novel findings can pave the way for future studies aiming at the elucidation of the differentiation of multinucleated osteoclasts.</p

Future perspectives on sustainable tribology

AbstractThis paper highlights the future perspectives of sustainable tribology by examining the economic, environmental and social impact of three tribological case studies. One case study examines the sustainability and durability of micro-CHP systems looking the tribological phenomena generated within a scroll expander system. The scroll is the main part of a specific micro-CHP system and experiences wear and cavitation damage. The tribological optimization of the scroll expander improves the sustainability of the micro-CHP unit while it has a serious economic and environmental impact to the consumers and to the society in general. Another case study is focused on friction and wear performance of lifeboat launch slipways. The causes of high friction and wear during the RNLI's lifeboat launches along an inclined slipway are investigated with a view to reducing the environmental impact due to slipway panel wear and lubricant release into the marine environment. The project encompasses the sustainable design of slipway panels using design modifications based on tribological investigations to double their lifespan, while environmental and economic impact was significantly reduced by the use of biodegradable greases and water as lubricants. The final case study involves an investigation of recycled plastic materials to replace polyurethane used on skateboard wheels, scooters and similar applications. Polyurethane (PU) is difficult to recycle. With the dwindling resources and environmental problems facing the world today, recycling for both waste reduction and resource preservation has become an increasingly important aspect of sustainability. The tribological results showed that recycled polycarbonate plastic can effectively act as a substitute to polyurethane wheels. Moreover, sustainability considerations showing the environmental benefits of the use of recycled plastics over PU include reducing the CO2 footprint by 50% and the energy consumed by 60%, among other benefits. These case studies emphasise the importance of sustainable tribology in our epoch showing that increased sustainability performance can be achieved through tribology to a significant extent in many cases, providing stability to our world and more viable long term growth to our societies

Sp(4) gauge theory on the lattice: towards SU(4)/Sp(4) composite Higgs (and beyond)

The Sp(4) gauge theory with two Dirac fundamental flavours provides a candidate for the microscopic origin of composite-Higgs models based on the SU(4)/Sp(4) coset. We employ a combination of two different, complementary strategies for the numerical lattice calculations, based on the Hybrid Monte Carlo and on the Heat Bath algorithms. We perform pure Yang-Mills, quenched computations and exploratory studies with dynamical Wilson fermions. We present the first results in the literature for the spectrum of glueballs of the pure Sp(4) Yang-Mills theory, an EFT framework for the interpretation of the masses and decay constants of the lightest pion, vector and axial-vector mesons, and a preliminary calculation of the latter in the quenched approximation. We show the first numerical evidence of a bulk phase transition in the lattice theory with dynamical Wilson fermions, and perform the technical steps necessary to set up future investigations of the mesonic spectrum of the full theory

Symmetry restoration at high-temperature in two-color and two-flavor lattice gauge theories

We consider the SU(2) gauge theory with Nf=2 flavors of Dirac fundamental fermions. We study the high-temperature behavior of the spectra of mesons, discretizing the theory on anisotropic lattices, and measuring the two-point correlation functions in the temporal direction as well as screening masses in various channels. We identify the (pseudo-)critical temperature as the temperature at which the susceptibility associated with the Polyakov loop has a maximum. At high temperature both the spin-1 and spin-0 sectors of the light meson spectra exhibit enhanced symmetry properties, indicating the restoration of both the global SU(4) and the axial U(1)A symmetries of the model

A second Higgs doublet in the early universe: baryogenesis and gravitational waves

We show that simple Two Higgs Doublet models still provide a viable explanation for the matter-antimatter asymmetry of the Universe via electroweak baryogenesis, even after taking into account the recent order-of-magnitude improvement on the electron-EDM experimental bound by the ACME Collaboration. Moreover we show that, in the region of parameter space where baryogenesis is possible, the gravitational wave spectrum generated at the end of the electroweak phase transition is within the sensitivity reach of the future space-based interferometer LISA

Health-related quality of life change in patients treated at a multidisciplinary pain clinic

Background Multidisciplinary pain management (MPM) is a generally accepted method for treating chronic pain, but heterogeneous outcome measures provide only limited conclusions concerning its effectiveness. Therefore, further studies on the effectiveness of MPM are needed to identify subgroups of patients who benefit, or do not benefit, from these interventions. Our aim was to analyse health-related quality of life (HRQoL) changes after MPM and to identify factors associated with treatment outcomes. Methods We carried out a real world observational follow-up study of chronic pain patients referred to a tertiary multidisciplinary outpatient pain clinic to describe, using the validated HRQoL instrument 15D, the HRQoL change after MPM and to identify factors associated with this change. 1,043 patients responded to the 15D HRQoL questionnaire at baseline and 12 months after the start of treatment. Background data were collected from the pre-admission questionnaire of the pain clinic. Results Fifty-three percent of the patients reported a clinically important improvement and, of these, 81% had a major improvement. Thirty-five percent reported a clinically important deterioration, and 12% had no change in HRQoL. Binary logistic regression analysis revealed that major improvement was positively associated with shorter duration of pain (Peer reviewe

Determinants of DNA yield and purity collected with buccal cell samples

Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 μg (median 54.3 μg; mean 82.2 μg ± SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 μg) than those from women (median 44.2 μg). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics