Plastid lipid droplets at the crossroads of prenylquinone metabolism

Lipid droplets called plastoglobules (PGs) exist in most plant tissues and plastid types. In chloroplasts, the polar lipid monolayer surrounding these low-density lipoprotein particles is continuous with the outer lipid leaflet of the thylakoid membrane. Often small clusters of two or three PGs, only one of them directly connected to thylakoids, are present. Structural proteins (known as plastid-lipid associated proteins/fibrillins or plastoglobulins) together with lipid metabolic enzymes coat the PGs. The hydrophobic core of PGs contains a range of neutral lipids including the prenylquinones [tocopherols (vitamin E), phylloquinone (vitamin K1), and plastoquinone (PQ-9)]. In this review the function of PGs and their associated enzymes in prenylquinone metabolism will be discusse

Predicted Extension of the Sagittarius Stream to the Milky Way Virial Radius

The extensive span of the Sagittarius (Sgr) stream makes it a promising tool for studying the Milky Way gravitational potential. Characterizing its stellar kinematics can constrain halo properties and provide a benchmark for the Cold Dark Matter galaxy formation paradigm. Accurate models of the disruption dynamics of the Sgr progenitor are necessary to employ this tool. Using a combination of analytic modeling and N-body simulations, we build a new model of the Sgr orbit that produces an unprecedentedly good fit to observations. In contrast to previous models, we simulate the full infall trajectory of the Sgr progenitor from the time it first crossed the Milky Way virial radius 8 Gyr ago. An exploration of the parameter space of initial phase-space conditions yields tight constraints on the angular momentum of the Sgr progenitor. Our best-fit model is the first to reproduce accurately existing data on the 3D positions and radial velocities of the debris detected 100 kpc away in the MW halo. In addition to replicating the mapped stream, the simulation also predicts the existence of several arms of the Sgr stream extending to hundreds of kiloparsecs. The two most distant stars known in the Milky Way halo coincide with the predicted structure. Additional stars in the newly predicted arms can be found with future data from the Large Synoptic Survey Telescope. Detecting a statistical sample of stars in the most distant Sgr arms would provide an opportunity to constrain the Milky Way potential out to unprecedented Galactocentric radii.Astronom

The contrasting origins of glauconite in the shallow marine environment highlight this mineral as a marker of paleoenvironmental conditions

Glauconite is an authigenic mineral reputed to form during long-lasting contact between a nucleus (a pre-existing phyllosilicate) and seawater. This protracted contact makes it possible to subtract the ions necessary for the construction of the neoformed phyllosilicate, here, glauconite (a mineral very close to an illite, rich in K and Fe). As a result, glauconite is often associated with sediments deposited in a transgressive context with a strong slowdown in the rate of sedimentation and a relatively large water layer thickness. This is the case of the Cenomanian chalk of Boulonnais (north of France). Being chemically and physically resistant, glauconite is a mineral that is often reworked, like quartz grains. This is frequently the case of the Jurassic deposits of the Boulonnais, where glauconite, almost ubiquitous, either in traces or in significant proportions of the sediments, presents a grain size sorting attesting to its transport and reworking. However, these Jurassic deposits are shallow (shoreface, upper offshore), which supports the idea that the “glauconite factory” was itself in the shallow areas of the Boulonnais. The only identified Jurassic facies of the Boulonnais where glauconite is both relatively abundant, large in size and unsorted (non reworked) are oyster reefs that formed at the outlet of cold seeps linked to a late-Jurassic synsedimentary tectonic (Kimmeridgian, Tithonian). Our work makes it possible to hypothesize that isolated oyster reefs were environments combining the redox conditions and in contact with seawater favoring the authigenic formation of glauconite. The weakly reducing conditions necessary for the formation of glauconite here are attested by the contents of metallic trace elements sensitive to redox conditions (vanadium, germanium, arsenic, in this case). Our work thus adds a new element to the understanding of the mechanisms of formation of glauconite in shallow environments

A novel approach to volcano surveillance using gas geochemistry

The aim of this paper is to test a simple damage model of a cohesive granular medium to study the relationship between the damage and velocity of elastic waves. Our numerical experiments of edometric compression show that the mi- croscopic deformation quickly becomes very heterogeneous, while our simulations of elastic waves propagation show that a small amount of damage induces a dra- matic decrease in the elastic velocity. This shows that cohesive discrete media are very sensitive to strain field heterogeneity, and that the wave velocities in these media can measure subtle transient deformation processes, such as earthquake initiation phases

The feasibility of using pedometers and brief advice to increase activity in sedentary older women:a pilot study

Background: People over the age of 70 carry the greatest burden of chronic disease, disability and health care use. Participation in physical activity is crucial for health, and walking accounts for much of the physical activity undertaken by sedentary individuals. Pedometers are a useful motivational tool to encourage increased walking and they are cheap and easy to use. The aim of this pilot study was to evaluate the feasibility of the use of pedometers plus a theory-based intervention to assist sedentary older women to accumulate increasing amounts of physical activity, mainly through walking. Methods: Female participants over the age of 70 were recruited from primary care and randomised to receive either pedometer plus a theory-based intervention or a theory-based intervention alone. The theory-based intervention consisted of motivational techniques, goal-setting, barrier identification and self-monitoring with pedometers and daily diaries. The pedometer group were further randomised to one of three target groups: a 10%, 15% or 20% monthly increase in step count to assess the achievability and acceptability of a range of targets. The primary outcome was change in daily activity levels measured by accelerometry. Secondary outcome measures were lower limb function, health related quality of life, anxiety and depression. Results: 54 participants were recruited into the study, with an average age of 76. There were 9 drop outs, 45 completing the study. All participants in the pedometer group found the pedometers easy to use and there was good compliance with diary keeping (96% in the pedometer group and 83% in the theory-based intervention alone group). There was a strong correlation (0.78) between accelerometry and pedometer step counts i.e. indicating that walking was the main physical activity amongst participants. There was a greater increase in activity (accelerometry) amongst those in the 20% target pedometer group compared to the other groups, although not reaching statistical significance (p = 0.192). Conclusion: We have demonstrated that it is feasible to use pedometers and provide theory-based advice to community dwelling sedentary older women to increase physical activity levels and a larger study is planned to investigate this further.Publisher PDFPeer reviewe

Population-based screening in a municipality after a primary school outbreak of the SARSCoV-2 Alpha variant, the Netherlands, December 2020–February 2021

An outbreak of SARS-CoV-2 Alpha variant (Pango lineage B.1.1.7) was detected at a primary school (School X) in Lansingerland, the Netherlands, in December 2020. The outbreak was studied retrospectively, and population-based screening was used to assess the extent of virus circulation and decelerate transmission. Cases were SARS-CoV-2 laboratory confirmed and were residents of Lansingerland (November 16(th) 2020 until February 22(th) 2021), or had an epidemiological link with School X or neighbouring schools. The SARS-CoV-2 variant was determined using variant PCR or whole genome sequencing. A questionnaire primarily assessed clinical symptoms. A total of 77 Alpha variant cases were found with an epidemiological link to School X, 16 Alpha variant cases linked to the neighbouring schools, and 146 Alpha variant cases among residents of Lansingerland without a link to the schools. The mean number of self-reported symptoms was not significantly different among Alpha variant infected individuals compared to non-Alpha infected individuals. The secondary attack rate (SAR) among Alpha variant exposed individuals in households was 52% higher compared to non-Alpha variant exposed individuals (p = 0.010), with the mean household age, and mean number of children and adults per household as confounders. Sequence analysis of 60 Alpha variant sequences obtained from cases confirmed virus transmission between School X and neighbouring schools, and showed that multiple introductions of the Alpha variant had already taken place in Lansingerland at the time of the study. The alpha variant caused a large outbreak at both locations of School X, and subsequently spread to neighbouring schools, and households. Population-based screening (together with other public health measures) nearly stopped transmission of the outbreak strain, but did not prevent variant replacement in the Lansingerland municipality