Esperimento RTK1: verifica delle prestazioni del posizionamento NRTK (Cap. 4 app. 1)

Un libro bianco su: "I servizi di posizionamento satellitare per l'e-government

the tempio della consolazione in todi integrated geomatictechniques for a monument description including structuraldamage evolution in time

The Tempio della Consolazione in Todi (16th cent.) has always been one of the most significant symbols of the Umbrian landscape. Since the first times after its completion (1606) the structure has exhibited evidences of instability, due to foundation subsiding and/or seismic activity. Structural and geotechnical countermeasures have been undertaken on the Tempio and its surroundings from the 17th century until recent times. Until now a truly satisfactory analysis of the overall deformation and attitude of the building has not been performed, since the existing surveys record the overhangs of the pillars, the crack pattern or the subsidence over limited time spans. Describing the attitude of the whole church is in fact a complex operation due to the architectural character of the building, consisting of four apses (three polygonal and one semicircular) covered with half domes, which surround the central area with the large dome. The present research aims to fill the gap of knowledge with a global study based on geomatic techniques for an accurate 3D reconstruction of geometry and attitude, integrated with a historical research on damage and interventions and a geotechnical analysis. The geomatic survey results from the integration of different techniques: GPS-GNSS for global georeferencing, laser scanning and digital photogrammetry for an accurate 3D reconstruction, high precision total station and geometric leveling for a direct survey of deformations and cracks, and for the alignment of the laser scans. The above analysis allowed to assess the dynamics of the cracks occurred in the last 25 years by a comparison with a previous survey. From the photographic colour associated to the point cloud was also possible to map the damp patches showing on the domes intrados, mapping their evolution over the last years

Esperimento RTK1: verifica delle prestazioni del posizionamento NRTK (Cap. 4 app. 1)

Un libro bianco su: "I servizi di posizionamento satellitare per l'e-government

Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses

Synaptic vesicle (SV) exocytosis mediating neurotransmitter release occurs spontaneously at low intraterminal calcium concentrations and is stimulated by a rise in intracellular calcium. Exocytosis is compensated for by the reformation of vesicles at plasma membrane and endosomes. Although the adaptor complex AP-3 was proposed to be involved in the formation of SVs from endosomes, whether its function has an indirect effect on exocytosis remains unknown. Using mocha mice, which are deficient in functional AP-3, we identify an AP-3-dependent tetanus neurotoxin-resistant asynchronous release that can be evoked at hippocampal mossy fiber (MF) synapses. Presynaptic targeting of the tetanus neurotoxin-resistant vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) is lost in mocha hippocampal MF terminals, whereas the localization of synaptobrevin 2 is unaffected. In addition, quantal release in mocha cultures is more frequent and more sensitive to sucrose. We conclude that lack of AP-3 results in more constitutive secretion and loss of an asynchronous evoked release component, suggesting an important function of AP-3 in regulating SV exocytosis at MF terminals

Integrated Geomatic Techniques for Georeferencing and Reconstructing the Position of Underground Archaeological Sites: The Case Study of the Augustus Sundial (Rome)

A large part of the archaeological remains still to be discovered and excavated are not in remote and depopulated areas of the earth but are often beneath urban centres that have buried them with centuries of debris and later constructions. Excavating in these contexts is much more complex than digging in rural or sparsely inhabited areas because of the constraints imposed by existing buildings and infrastructure. It should also be considered that within an urbanised area, any archaeological remains are concentrated in the subsoil of the historic centre, which is, therefore, often surmounted by buildings that are more recent than the remains but historical as well, and thus, of considerable value and vulnerability. For this reason, an archaeological excavation in an urban area must be preceded by a real feasibility study, where the potential risks for the structures above are minimised and accurately quantified. In many situations, as in the case under study, the discovery of a small segment of a structure is the only clue to reconstruct the development of the remaining part still to be excavated, which may stretch tens or hundreds of metres away from the measurable part. As a consequence, an error of a few centimetres in the survey of the excavated part can lead to errors of metres in estimating the positions of the far parts still to be excavated, and this, in many cases, as in the one under study, must absolutely be avoided. In practice, high-precision geomatic surveys, in support of the archaeological and historical interpretation of the observable structures, will help to establish the exact locations to possibly continue the excavations, helping the accurate planning of the excavation itself. Here, we have shown how the various techniques, compared to each other, have made it possible to reconstruct the location of a short stretch (less than 7 metres) of the Emperor Augustus' Sundial, the only currently visible evidence of a scientific instrument of imposing dimensions (tens of metres in length and height) that served to define some of the characteristics of the calendar that we still use today. The portion of the sundial currently observable, according to the most reliable hypotheses, is located approximately at one end of a structure and extends for several tens of metres. The accurate positioning of the observable parts in a geodetic reference system will enable to identify with certainty the possible areas in which excavation may continue and will also allow to accurately reconstruct the principle of operation of the sundial through an approach that could be defined as "reverse engineering" of the scientific instrument itself. The aim of this work is to study and thus define the combination and integration of existing geomatic techniques for this specific field of applicatio

A specific nanobody prevents amyloidogenesis of D76N β2-microglobulin in vitro and modifies its tissue distribution in vivo

Systemic amyloidosis is caused by misfolding and aggregation of globular proteins in vivo for which effective treatments are urgently needed. Inhibition of protein self-aggregation represents an attractive therapeutic strategy. Studies on the amyloidogenic variant of β2-microglobulin, D76N, causing hereditary systemic amyloidosis, have become particularly relevant since fibrils are formed in vitro in physiologically relevant conditions. Here we compare the potency of two previously described inhibitors of wild type β2-microglobulin fibrillogenesis, doxycycline and single domain antibodies (nanobodies). The β2-microglobulin -binding nanobody, Nb24, more potently inhibits D76N β2-microglobulin fibrillogenesis than doxycycline with complete abrogation of fibril formation. In β2-microglobulin knock out mice, the D76N β2-microglobulin/ Nb24 pre-formed complex, is cleared from the circulation at the same rate as the uncomplexed protein; however, the analysis of tissue distribution reveals that the interaction with the antibody reduces the concentration of the variant protein in the heart but does not modify the tissue distribution of wild type β2-microglobulin. These findings strongly support the potential therapeutic use of this antibody in the treatment of systemic amyloidosis

A closer look at neuron interaction with track-etched microporous membranes

Microporous membranes support the growth of neurites into and through micro-channels, providing a different type of neural growth platform to conventional dish cultures. Microporous membranes are used to support various types of culture, however, the role of pore diameter in relation to neurite growth through the membrane has not been well characterised. In this study, the human cell line (SH-SY5Y) was differentiated into neuron-like cells and cultured on track-etched microporous membranes with pore and channel diameters selected to accommodate neurite width (0.8 µm to 5 µm). Whilst neurites extended through all pore diameters, the extent of neurite coverage on the non-seeded side of the membranes after 5 days in culture was found to be directly proportional to channel diameter. Neurite growth through membrane pores reduced significantly when neural cultures were non-confluent. Scanning electron microscopy revealed that neurites bridged pores and circumnavigated pore edges – such that the overall likelihood of a neurite entering a pore channel was decreased. These findings highlight the role of pore diameter, cell sheet confluence and contact guidance in directing neurite growth through pores and may be useful in applications that seek to use physical substrates to maintain separate neural populations whilst permitting neurite contact between cultures