Buried and accessible surface area control intrinsic protein flexibility

Proteins experience a wide variety of conformational dynamics that can be crucial for facilitating their diverse functions. How is the intrinsic flexibility required for these motions encoded in their three-dimensional structures? Here, the overall flexibility of a protein is demonstrated to be tightly coupled to the total amount of surface area buried within its fold. A simple proxy for this, the relative solvent accessible surface area (Arel), therefore shows excellent agreement with independent measures of global protein flexibility derived from various experimental and computational methods. Application of Arel on a large scale demonstrates its utility by revealing unique sequence and structural properties associated with intrinsic flexibility. In particular, flexibility as measured by Arel shows little correspondence with intrinsic disorder, but instead tends to be associated with multiple domains and increased {\alpha}- helical structure. Furthermore, the apparent flexibility of monomeric proteins is found to be useful for identifying quaternary structure errors in published crystal structures. There is also a strong tendency for the crystal structures of more flexible proteins to be solved to lower resolutions. Finally, local solvent accessibility is shown to be a primary determinant of local residue flexibility. Overall this work provides both fundamental mechanistic insight into the origin of protein flexibility and a simple, practical method for predicting flexibility from protein structures.Comment: 36 pages, 11 figures, author's manuscript, accepted for publication in Journal of Molecular Biolog

Asset Pricing Model Specification and the Term Structure Evidence

In this paper, a set of tests of models of relative capital asset pricesis developed. The tests are used to examine how well the models explain maturity premiums on Government bonds, though they are perfectly general and hence could be applied to stocks or other assets. Allowance is made in the tests for the nonobservability of investors' optimal per capita consumption (or expected marginal utility). It is found that the returns on Government bonds bear a systematic risk which is better measured by their covariability with aggregate per capita consumption than with the returns on the NYSE stock market index, the latter being the surrogate-wealth portfolio typically used to measure risk in the traditional Sharpe-Lintner-Mossin CAPM.

A practical approach to the patient presenting with dropped head

Head drop, or having a dropped head, is an uncommon condition in which patients present with a disabling inability to lift their head. It may arise in many neurological conditions that can be divided into those with neuromuscular weakness of neck extensors and those with increased tone of neck flexors. The most common neuromuscular causes include myasthenia gravis, motor neurone disease and myositis, while neck dystonia secondary to movement disorders can cause an increased tone. Investigations should include blood tests, imaging, muscle biopsy and neurophysiological studies. Here, we present a man with dropped head and discuss the investigation and management of this condition.peer-reviewe

Service of process in the United States under Insolvency Rule 12.12

Explains the procedure to be followed by a UK based liquidator wishing to serve insolvency proceedings on parties based in the US under the Insolvency Rules 1986 r.12.12. Discusses the rule's requirements for obtaining leave of the court for service outside the jurisdiction, the court's discretion to order the manner of service, including the approach of the Chancery Division in Re Busytoday Ltd, and the obligations imposed by the Hague Convention on the Service Abroad of Judicial and Extrajudicial Documents in Civil or Commercial Matters 1965. Evaluates the methods of service authorised by the Convention and details the main elements involved in service by the Central Authority route or by a local process server

Dissipative particle dynamics: the equilibrium for finite time steps

Dissipative particle dynamics (DPD) is a relatively new technique which has proved successful in the simulation of complex fluids. We caution that for the equilibrium achieved by the DPD simulation of a simple fluid the temperature depends strongly on the time step. An analytic expression for the dependence is obtained and shown to agree well with simulation results.Comment: 5 pages, LaTeX, 1 Postscript figure, submitted to Europhys.Letts., Algebraic corrections made to final resul

Surprise volume and heteroskedasticity in equity market returns

Heterosedasticity in returns may be explainable by trading volume. We use different volume variables, including surprise volume - i.e. unexpected above-avergae trading activity - which is derived from uncorrelated volume innovations. Assuming eakly exogenous volume, we extend the Lamoureux and Lastrapes (1990) model by an asymmetric GARCH in-mean specification following Golstein et al. (1993). Model estimation for the U.S. as well as six large equity markets shows that surprise volume superior model fit and helps to explain volatility persistence as well as excess kurtosis. Surprise volume reveals a significant positive market risk premium, asymmetry, and a surprise volume effect in conditional variance. The findings suggest that, e.g., a surprise volume shock (breakdown) - i.e. large (small) contemporaneous and small (large) lagged surprise volume - relates to increased (decreased) conditional market variance and return. --ARCH,trading volume,return volume dependence,asymmetric volatility,market risk premium,leverage effect

Surprise Volume and Heteroskedasticity in Equity Market Returns

Heteroskedasticity in returns may be explainable by trading volume. We use different volume variables, including surprise volume---i.e. unexpected above-average trading activity---which is derived from uncorrelated volume innovations. Assuming weakly exogenous volume, we extend the Lamoureux and Lastrapes (1990) model by an asymmetric GARCH in-mean specification following Golsten et al. (1993). Model estimation for the U.S. as well as six large equity markets shows that surprise volume provides superior model fit and helps to explain volatility persistence as well as excess kurtosis. Surprise volume reveals a significant positive market risk premium, asymmetry, and a surprise volume effect in conditional variance. The findings suggest that, e.g., a surprise volume shock (breakdown)---i.e. large (small) contemporaneous and small (large) lagged surprise volume---relates to increased (decreased) conditional market variance and return.ARCH, trading volume, return volume dependence, asymmetric volatility, market risk premium, leverage effect

A Young Planetary-Mass Object in the ρ Oph Cloud Core

We report the discovery of a young planetary-mass brown dwarf in the ρ Oph cloud core. The object was identified as such with the aid of a 1.5-2.4 μm low-resolution spectrum obtained using the NIRC instrument on the Keck I telescope. Based on the COND model, the observed spectrum is consistent with a reddened (A_V ~ 15-16) brown dwarf whose effective temperature is in the range 1200-1800 K. For an assumed age of 1 Myr, comparison with isochrones further constrains the temperature to ~1400 K and suggests a mass of ~2-3 Jupiter masses. The inferred temperature is suggestive of an early T spectral type, which is supported by spectral morphology consistent with weak methane absorption. Based on its inferred distance (~100 pc) and the presence of overlying visual absorption, it is very likely to be a ρ Oph cluster member. In addition, given the estimated spectral type, it may be the youngest and least massive T dwarf found so far. Its existence suggests that the initial mass function for the ρ Oph star-forming region extends well into the planetary-mass regime

The Effect of Injection-Valve Opening Pressure on Spray-Tip Penetration

The effect of various injection-valve opening pressures on the spray-tip penetration was determined for several injection pressure. A common-rail fuel injection system was used. For a given injection pressure a maximum rate of penetration was obtained with an injection-valve opening pressure equal to the injection pressure. As the excess of the injection pressure over the injection-valve opening pressure was increased for a given injection pressure, the effect of the injection-valve opening pressure on the spray-tip penetration was increased