Antennal Depression as a Consummatory Response in the Madagascar Hissing Cockroach

Sign-tracking is a counterintuitive response that occurs when a signal stimulus is paired with reinforcement. Sign- and goal-tracking studies have been replicated across several species including pigeons, rats, quail, and cuttlefish. To date, no sign-tracking studies with the Madagascar Hissing Cockroach (MHC) are in the literature. Replicating sign-tracking studies with this new species is essential to an understanding how this counter-intuitive behavior occurs, particularly in invertebrate species like the MHC. To establish research on MHC sign- and goal-tracking, however, will first require ethological studies to identify potential dependent variables that indicate MHC responses to meaningful versus neutral stimuli. This study identifies the response topography of consummatory behavior in the MHC, for use with future studies

ExoMars Atmospheric Science and Missions Workshop Abstracts

The “ExoMars Atmospheric Science and Missions” Workshop served as a forum for general discussions on Martian atmospheric science with a focus on the assessment of the results and instrumentation development cycle of the ExoMars 2016 mission. These led to presentations and discussions of the atmospheric investigation plans and strategies for the ESA ExoMars-2020 mission in particular and for forthcoming Mars missions in general. The workshop gave overviews of the ExoMars atmospheric investigations through invited talks by Exomars scientists. The ExoMars atmospheric results and planned investigations were covered by individual scientific presentations. The workshop engaged early career scientists, inclusiveness states and scientific and technological cooperation in the European planetary science community. The Workshop provided a forum for discussion and debate on the outstanding scientific topics of the Martian atmosphere, and on how to integrate and network the scientific teams with providers of instruments and technical systems. Thus the workshop also contributed to international cooperation in the field of Martian atmospheric science and technology

Modeling binding specificities of transcription factor pairs with random forests

Background Transcription factors (TFs) bind regulatory DNA regions with sequence specificity, form complexes and regulate gene expression. In cooperative TF-TF binding, two transcription factors bind onto a shared DNA binding site as a pair. Previous work has demonstrated pairwise TF-TF-DNA interactions with position weight matrices (PWMs), which may however not sufficiently take into account the complexity and flexibility of pairwise binding. Results We propose two random forest (RF) methods for joint TF-TF binding site prediction: ComBind and JointRF. We train models with previously published large-scale CAP-SELEX DNA libraries, which comprise DNA sequences enriched for binding of a selected TF pair. JointRF builds a random forest with sub-sequences selected from CAP-SELEX DNA reads with previously proposed pairwise PWM. JointRF outperforms (area under receiver operating characteristics curve, AUROC, 0.75) the current state-of-the-art method i.e. orientation and spacing specific pairwise PWMs (AUROC 0.59). Thus, JointRF may be utilized to improve prediction accuracy for pre-determined binding preferences. However, pairwise TF binding is currently considered flexible; a pair may bind DNA with different orientations and amounts of dinucleotide gaps or overlap between the two motifs. Thus, we developed ComBind, which utilizes random forests by considering simultaneously multiple orientations and spacings of the two factors. Our approach outperforms (AUROC 0.78) PWMs, as well as JointRF (pPeer reviewe

Measurement of Martian boundary layer winds by the displacement of jettisoned lander hardware

Abstract Martian boundary layer wind speed and direction measurements, from a variety of locations, seasons and times, are provided. For each lander sent to Mars over the last four decades a unique record of the winds blowing during their descent is preserved at each landing site. By comparing images acquired from orbiting spacecraft of the impact points of jettisoned hardware, such as heat shields and parachutes, to a trajectory model the winds can be measured. We start our investigations with the Viking lander 1 mission and end with Schiaparelli. In-between we extract wind measurements based on observations of the Beagle 2, Spirit, Opportunity, Phoenix and Curiosity landing sites. With one exception the wind at each site during the lander’s descent were found to be   20 m s − 1 ), blowing from the north-west was required at a high altitude ( > 2 km) together with a gust close to the surface ( < 500 m altitude) originating from the north. All in all our investigations yielded a total of ten unique wind measurements in the PBL. One each from the Viking landers and one each from Beagle 2, Spirit, Opportunity and Schiaparelli. Two wind measurements, one above about 1 km altitude and one below, were possible from observations of the Curiosity and Phoenix landing site. Our findings are consistent with a turbulent PBL in the afternoon and calm PBL in the morning. When comparing our results to a GCM we found a good match in wind direction but not for wind speed. The information provided here makes available wind measurements previously unavailable to Mars atmosphere modellers and investigators.Peer reviewe

NMR Structure Determinations of Small Proteins Using only One Fractionally 20% 13C- and Uniformly 100% 15N-Labeled Sample

Uniformly 13C- and 15N-labeled samples ensure fast and reliable nuclear magnetic resonance (NMR) assignments of proteins and are commonly used for structure elucidation by NMR. However, the preparation of uniformly labeled samples is a labor-intensive and expensive step. Reducing the portion of 13C-labeled glucose by a factor of five using a fractional 20% 13C- and 100% 15N-labeling scheme could lower the total chemical costs, yet retaining sufficient structural information of uniformly [13C, 15N]-labeled sample as a result of the improved sensitivity of NMR instruments. Moreover, fractional 13C-labeling can facilitate reliable resonance assignments of sidechains because of the biosynthetic pathways of each amino-acid. Preparation of only one [20% 13C, 100% 15N]-labeled sample for small proteins

NMR Structure Determinations of Small Proteins Using only One Fractionally 20% 13C- and Uniformly 100% 15N-Labeled Sample

Uniformly 13C- and 15N-labeled samples ensure fast and reliable nuclear magnetic resonance (NMR) assignments of proteins and are commonly used for structure elucidation by NMR. However, the preparation of uniformly labeled samples is a labor-intensive and expensive step. Reducing the portion of 13C-labeled glucose by a factor of five using a fractional 20% 13C- and 100% 15N-labeling scheme could lower the total chemical costs, yet retaining sufficient structural information of uniformly [13C, 15N]-labeled sample as a result of the improved sensitivity of NMR instruments. Moreover, fractional 13C-labeling can facilitate reliable resonance assignments of sidechains because of the biosynthetic pathways of each amino-acid. Preparation of only one [20% 13C, 100% 15N]-labeled sample for small proteins

Simultaneous Triggered Collapse of the Presolar Dense Cloud Core and Injection of Short-Lived Radioisotopes by a Supernova Shock Wave

Cosmochemical evidence for the existence of short-lived radioisotopes (SLRI) such as $^{26}$Al and $^{60}$Fe at the time of the formation of primitive meteorites requires that these isotopes were synthesized in a massive star and then incorporated into chondrites within $\sim 10^6$ yr. A supernova shock wave has long been hypothesized to have transported the SLRI to the presolar dense cloud core, triggered cloud collapse, and injected the isotopes. Previous numerical calculations have shown that this scenario is plausible when the shock wave and dense cloud core are assumed to be isothermal at $\sim 10$ K, but not when compressional heating to $\sim 1000$ K is assumed. We show here for the first time that when calculated with the FLASH2.5 adaptive mesh refinement (AMR) hydrodynamics code, a 20 km/sec shock wave can indeed trigger the collapse of a 1 $M_\odot$ cloud while simultaneously injecting shock wave isotopes into the collapsing cloud, provided that cooling by molecular species such as H$_2$O, CO$_2$, and H$_2$ is included. These calculations imply that the supernova trigger hypothesis is the most likely mechanism for delivering the SLRI present during the formation of the solar system.Comment: 12 pages, 4 color figures. Astrophysical Journal Letters (in press