Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis

The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects. Therefore, we conclude that the assay could be incorporated into the laboratory routine where flow cytometry is applied for semen quality control.Peer reviewe

THREE PHASE RECTIFICATION WITH HIGH FREQUENCY PULSES

The three phase full bridge thyristor rectifier has drawbacks. The low power factor, the high harmonic content of the dc voltage at high firing angle and the inherent dead time are the main reasons for discomfort. The configuration suggested here offers some remedy for all three disadvantages. High frequency sinusoidal current pulses are delivering the energy from the input to the output here. The paper describes the mode of action of the configuration. Symmetrical components are used for the mathematical analysis. Pulse distribution ensuring unit power factor in the whole operation range is suggested. Diodes are applied for clamping at the end of the paper. This is the simplest realization of the concept

Studying star-forming processes at core and clump scales: the case of the young stellar object G29.862-0.0044

Massive molecular clumps fragment into cores where star formation takes place, hence star-forming studies should be done at different spatial scales. Using near-IR data obtained with Gemini, data of CH3OCHO and CH3CN from the ALMA database, observations of HCN, HNC, HCO+, and C2H carried out with ASTE, and CO data from public surveys, we perform a deep study of the YSO G29.86-0.004 at core and clump spatial scales. The near-IR emission shows two nebulosities separated by a dark lane, suggesting a typical disk-jets system, but highly asymmetric. They are likely produced by scattered light in cavities carved out by jets on an infalling envelope of material, which also present line emission of H2 and [FeII]. The presence of the complex molecular species observed with ALMA confirms that we are mapping a hot molecular core. The CH3CN emission concentrates at the position of the dark lane and it appears slightly elongated from southwest to northeast in agreement with the inclination of the system as observed at near-IR. The morphology of the CH3OCHO emission is more complex and extends along some filaments and concentrates in knots and clumps, mainly southwards the dark-lane, suggesting that the southern jet is encountering a dense region. The northern jet flows more freely, generating more extended features. This is in agreement with the red-shifted molecular outflow traced by the 12CO J=3-2 line extending towards the northwest and the lack of a blue-shifted outflow. This configuration can be explained by considering that the YSO is located at the furthest edge of the molecular clump along the line of sight, which is consistent with the position of the source in the cloud mapped in the C18O J=3-2 line. The detection of HCN, HNC, HCO+, and C2H allowed us to characterize the dense gas at clump scales, yielding results that are in agreement with the presence of a high-mass protostellar object.Comment: Accepted to be published in A&A (July 13, 2020

The Effects Of Host Nutritional Status And Dietary Factors On The Treatment Efficacy Of Albendazole In School-Age Children Infected With Hookworm In The Kintampo North District Of Ghana

Background: In endemic settings unable to support economic growth, inexpensive anthelminthics, such as albendazole, are the cornerstone for hookworm infection treatment and control. However, the overall efficacy of albendazole against hookworm may be reduced after periodic chemotherapy. (Hotez et al., 2004) In addition, there are few studies exploring the role of nutritional status as a potential host factor influencing drug response and the constituents of diet has often been overlooked when determining anthelminthic efficacy, despite previous accounts of nutrient content of a meal or consumed food item affecting drug metabolism. Evaluating nutritional status as a potential host factor may partly explain the wide variability of drug efficacy. (Vercruysse et al., 2011) Methodology: Ghanaian school children that met inclusion criteria were selected from five communities previously identified as having high prevalence of hookworm infection. After enrolling 141 eligible school-age children for baseline assessment, those positive for hookworm infection were treated with single-dose 400 mg albendazole. Nutritional status and dietary data were assessed to identify modifiable host factors affecting treatment response. Principal Findings: Our results showed that the efficacy of single-dose oral albendazole for curing hookworm infection was significantly reduced for children with lower dietary diversity and protein intake on treatment day. In addition, children with higher dietary diversity scores within the same reference period were 3.08 times more likely than those with lower scores to experience the highest egg reduction rates (p-value 0.0430). Conclusion: The cross-sectional study provides new data on the nutritional status and dietary patterns of children in the Kintampo North Municipality and elucidates the potential role of modifiable host factors in affecting response to treatment

Neues zur Urbanistik der Zivilstädte von Aquincum-Budapest und Carnuntum-Petronell

In this paper the new results of an Austrian-Hungarian research cooperation (2011–2014) on the urbanism of the Civil towns of Aquincum and Carnuntum are to be presented. In synthesis of geophysical surveys, the interpretation of archaeological excavations and the reinterpretation of elder evidences, a new picture of the diachronic development of the two Civil Towns from an early vicus to a fortified city can be drawn

When and Where was Helen Zrinski Born?

Nakon pritvora Petra Zrinskog († 1670), kao što je poznato, arhiva obitelji bila je gotovo u potpunosti uništena. Kao rezultat toga nesigurnost je prevladavala kako u hrvatskoj, tako i u mađarskoj historiografiji, a vezano uz datume rođenja djece zavjereničkog aristokrata. Smatralo se da je sigurna samo godina rođenja Jelene Zrinske († 1703.). Prema podacima na njezinom nadgrobnom natpisu umrla je u progonstvu u Nikomediji (današnji Izmit) u dobi od 60 godina. Međutim, uzimajući u obzir događaje iz njezinog života, neki su mađarski istraživači s razlogom osporili izračunatu godinu 1643. kao godinu njezina rođenja. Oslanjajući se na objavljene i neobjavljene izvore, autor zaključuje da je Jelena Zrinska rođena kasnije, zapravo 1649. godine u Muraközu. Novootkriveni datum stavio je drugačiju perspektivu na glavne faze njezina turbulentnog tijeka života. Osim toga, ispostavilo se da Jelena Zrinska nije prvorođeno dijete Petra Zrinskog.Following Peter Zrinski’s (†1670) detention, as is well known, the archives of the family was almost completely destroyed. As a result, uncertainty has been dominating both the Croatian and the Hungarian historiography regarding the birth dates of the conspirant aristocrat’s children until now. Only Helen Zrinski’s (†1703) year of birth was thought to be certain. According to her epitaph she died in exile in Nicomedia (present-day Izmit) at the age of 60. Considering the events of her life, however, some Hungarian researchers have disputed the calculated year of 1643 as birth date with good reason. Relying on released and unpublished sources, the author concludes that Helen Zrinski was born later, in 1649 in Muraköz, actually. The newly discovered date put a different complexion on the major stages of her turbulent course of life. Besides, Helen Zrinski turned out not to be Peter Zrinski’s firstborn child

When and Where was Helen Zrinski Born?

Nakon pritvora Petra Zrinskog († 1670), kao što je poznato, arhiva obitelji bila je gotovo u potpunosti uništena. Kao rezultat toga nesigurnost je prevladavala kako u hrvatskoj, tako i u mađarskoj historiografiji, a vezano uz datume rođenja djece zavjereničkog aristokrata. Smatralo se da je sigurna samo godina rođenja Jelene Zrinske († 1703.). Prema podacima na njezinom nadgrobnom natpisu umrla je u progonstvu u Nikomediji (današnji Izmit) u dobi od 60 godina. Međutim, uzimajući u obzir događaje iz njezinog života, neki su mađarski istraživači s razlogom osporili izračunatu godinu 1643. kao godinu njezina rođenja. Oslanjajući se na objavljene i neobjavljene izvore, autor zaključuje da je Jelena Zrinska rođena kasnije, zapravo 1649. godine u Muraközu. Novootkriveni datum stavio je drugačiju perspektivu na glavne faze njezina turbulentnog tijeka života. Osim toga, ispostavilo se da Jelena Zrinska nije prvorođeno dijete Petra Zrinskog.Following Peter Zrinski’s (†1670) detention, as is well known, the archives of the family was almost completely destroyed. As a result, uncertainty has been dominating both the Croatian and the Hungarian historiography regarding the birth dates of the conspirant aristocrat’s children until now. Only Helen Zrinski’s (†1703) year of birth was thought to be certain. According to her epitaph she died in exile in Nicomedia (present-day Izmit) at the age of 60. Considering the events of her life, however, some Hungarian researchers have disputed the calculated year of 1643 as birth date with good reason. Relying on released and unpublished sources, the author concludes that Helen Zrinski was born later, in 1649 in Muraköz, actually. The newly discovered date put a different complexion on the major stages of her turbulent course of life. Besides, Helen Zrinski turned out not to be Peter Zrinski’s firstborn child